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Physical chemistry of isolated bacterial membranes

Biochimica et Biophysica Acta (BBA) - General Subjects, 1965
Abstract The membranes (plasma-mesosome system) isolated from Micrococcus lysodeekticus and Sarcina lutea have been dissociated with the non-ionic detergent, “Nonidet P.40” (active constituent of the polyoxyethylated alkyl phenol type), to give major components in the ultracentrifuge having sedimentation coefficients (sin20,w) of 3.1 and 3.3 ...
M R, Salton, A, Netschey
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ISOLATION AND CHARACTERIZATION OF MYELIN‐RELATED MEMBRANES

Journal of Neurochemistry, 1978
Abstract— Myelin related membrane fractions from rat brain and spinal cord were isolated from material normally discarded during standard myelin isolation procedures. A fraction which floated on 0.32 M‐sucrose (F) and the material released after subjecting the myelin fraction to osmotic shock at two stages in the purification (W1 and W2) were ...
R J, McIntyre   +3 more
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Junctional ultrastructure in isolated synaptic membranes

Journal of Neurocytology, 1975
The ultrastructure of synaptic junctions in whole brain tissue and isolated synaptic membranes has been compared. Type 1 junctions are present in the isolated membranes,readily identified by the presence of dense-staining material associated with the postsynaptic membrane, but the dense projections present at the presynaptic membrane in intact tissue ...
A I, Matus, B B, Walters, D H, Jones
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ATPase in isolated membranes of Bacillus subtilis

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1973
Abstract Cytoplasmic membranes from Bacillus subtilis ATCC 6633 were prepared by osmotic shock of protoplasts formed by the action of lysozyme. The resulting membrane ghosts were found to possess both Mg 2+ - and Ca 2+ -dependent ATP phosphohydrolase (ATPase, EC 3.6.1.3) activities.
S L, Rosenthal, A, Matheson
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Isolation and characterization of Chromatium vinosum membranes

Archives of Microbiology, 1974
A fractionation of Chromatium vinosum into an outer layer (cell wall) and three intracellular membrane fractions by isopycnic sucrose density centrifugation of a total membrane fraction obtained by lysis of lysozyme-EDTA spheroplasts is decribed.
Ronald E. Hurlbert   +2 more
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Isolation and characterization of chloroplast envelope membranes

1986
Publisher Summary This chapter discusses the isolation and characterization of chloroplast envelope membranes. The chloroplast envelope consists of a pair of lipid bilayer membranes. They form the interface between the stromal space of the chloroplast and the cytoplasm of the cell. The existence of two envelope membranes is not unique to chloroplasts
KENNETH KEEGSTRA, ABDULLAH E. YOUSIF
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Isolation of domains of the plasma membrane of hepatocytes

Experimental Cell Research, 1987
Several recent studies have demonstrated the ability of techniques based on immunoadsorption to selectively isolate specialized subregions of membranes, termed domains, which are derived from a larger more complex parent membrane like the plasma membrane.
J K, Petell   +4 more
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Isolation of the outer membrane of Branhamella catarrhalis

Microbial Pathogenesis, 1989
The emergence of Branhamella catarrhalis as an important human pathogen has stimulated interest in investigations of the outer membrane (OM) of the bacterium. In this study, the OM of B. catarrhalis was isolated and partially characterized. Radiolabelled cells were lysed and fractionated by isopycnic centrifugation in a continuous sucrose gradient ...
T F, Murphy, M R, Loeb
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Isolation of Protein Storage Vacuoles and Their Membranes

2016
Protein-storage vacuoles (PSVs) are specialized vacuoles that sequester large amounts of storage proteins. During seed development, PSVs are formed de novo and/or from preexisting lytic vacuoles. Seed PSVs can be subdivided into four distinct compartments: membrane, globoid, matrix, and crystalloid.
Tomoo, Shimada, Ikuko, Hara-Nishimura
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Isolating Extraembryonic Membranes

Cold Spring Harbor Protocols, 2006
Andras, Nagy   +3 more
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