Results 131 to 140 of about 94,650 (353)

Insight into microtubule disassembly by kinesin-13s from the structure of Kif2C bound to tubulin

Nature Communications, 2017
Kinesin-13s are microtubule depolymerizing enzymes. Here the authors present the crystal structure of a DARPin fused construct comprising the short neck region and motor domain of kinesin-13 in complex with an αβ-tubulin heterodimer, which shows that ...
Weiyi Wang, Soraya Cantos-Fernandes, Yuncong Lv, Hureshitanmu Kuerban, Shoeb Ahmad, Chunguang Wang, Benoît Gigant   +6 more
doaj   +1 more source

Making Two out of One: Kinesin Motors Driving Plant Cell Division

Cytoskeleton, EarlyView.
ABSTRACT The Kinesin superfamily of microtubule dependent motors is present in all eukaryotes. Not all of the subfamilies are represented in all kingdoms, and the ones that are do not always show conserved functions. Tight control of the cytoskeleton is essential for proper progression and completion of mitosis and cytokinesis, and key functions are ...
Choy Kriechbaum, Sabine Müller
wiley   +1 more source

Localization and function of Kinesin-5-like proteins during assembly and maintenance of mitotic spindles in Silvetia compressa

BMC Research Notes, 2009
Background Kinesin-5 (Eg-5) motor proteins are essential for maintenance of spindle bipolarity in animals. The roles of Kinesin-5 proteins in other systems, such as Arabidopsis, Dictyostelium, and sea urchin are more varied.
Miller Anne, Peters Nick T, Kropf Darryl L   +2 more
doaj   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Mechanism of Processive Movement of Monomeric and Dimeric Kinesin Molecules

International Journal of Biological Sciences, 2010
Kinesin molecules are motor proteins capable of moving along microtubule by hydrolyzing ATP. They generally have several forms of construct. This review focuses on two of the most studied forms: monomers such as KIF1A (kinesin-3 family) and dimers such ...
Ping Xie
doaj  

Cooperative Cargo Transport by Several Molecular Motors

, 2005
The transport of cargo particles which are pulled by several molecular motors in a cooperative manner is studied theoretically. The transport properties depend primarily on the maximal number, $N$, of motor molecules that may pull simultaneously on the ...
Ashkin, Bell, Block, B  hm, Erdmann, Goldstein, Hill, Kuznetsov, Lipowsky, Miller, R. Lipowsky, S. Klumpp, Schnitzer, Seitz, Shah, Svoboda, Trinczek, Vale, Visscher, Wang   +19 more
core   +2 more sources

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source

Spastic paraplegia mutation N256S in the neuronal microtubule motor KIF5A disrupts axonal transport in a Drosophila HSP model.

PLoS Genetics, 2012
Hereditary spastic paraplegias (HSPs) comprise a group of genetically heterogeneous neurodegenerative disorders characterized by spastic weakness of the lower extremities.
Petra Füger, Vrinda Sreekumar, Rebecca Schüle, Jeannine V Kern, Doychin T Stanchev, Carola D Schneider, Kathrin N Karle, Katharina J Daub, Vera K Siegert, Matthias Flötenmeyer, Heinz Schwarz, Ludger Schöls, Tobias M Rasse   +12 more
doaj   +1 more source

Regulation of kinesin activity by phosphorylation of kinesin-associated proteins.

Journal of Biological Chemistry, 1994
The mechanochemical motor proteins of the kinesin and cytoplasmic dynein families play important roles in microtubule-based intracellular motility. Although movement and distribution of organelles like secretory granules, vesicles, endoplasmic reticulum, and chromosomes depend on the activity of these motor proteins, little is known about the ...
James M. McIlvain, Janis K. Burkhardt, Sarah F. Hamm-Alvarez, Yair Argon, Michael P. Sheetz   +4 more
openaire   +3 more sources

Post‐LECA Origin and Diversification of an Axonemal Outer Arm Dynein Motor

Cytoskeleton, EarlyView.
ABSTRACT Dyneins were present in the last eukaryotic common ancestor (LECA) and play key roles in eukaryotic biology. Axonemal dyneins form the inner and outer arms that power ciliary beating, and it has long been recognized that outer arms in some organisms contain two different heavy chain motors, whereas those from other species contain a third unit
Stephen M. King
wiley   +1 more source