Results 61 to 70 of about 51,301 (248)

Tubocapsenolide A Overcomes Oxaliplatin Resistance in Colorectal Cancer by Targeting PLK1 to Impair CEP55–ESCRT‐Associated Vesicular Damage Clearance

Advanced Science, EarlyView.
Oxaliplatin‐resistant colorectal cancer cells utilize a previously unappreciated PLK1‐CEP55‐ESCRT‐driven vesicular extrusion mechanism to clear L‐OHP‐induced cellular damage. The natural withanolide Tubocapsenolide A directly targets and inhibits PLK1 to disrupt this specific damage‐clearance machinery. This blockade induces severe nucleolar stress and
Yueying Yang, Jing Liang, Lu Wang, Hongyan Zhang, Zhiruo Zhang, Yang Liu, Rui Zhu, Wen Xu, Shaohua Xu, Dejuan Sun, Ke Xiang, Yali Wang, Zhimin Fan, Zheng Wang, Hua Li, Lixia Chen   +15 more
wiley   +1 more source

DUSP26 Regulated by YY1 Lactylation Couples Osteogenesis and Angiogenesis by Dephosphorylating PMPCB at Y141 and Activating Mitophagy in BMSCs

Advanced Science, EarlyView.
p300‐associated YY1 lactylation promotes DUSP26 expression in BMSCs. By regulating PMPCB phosphorylation and PINK1 processing, DUSP26 supports mitophagy, osteogenic differentiation, and pro‐angiogenic factor secretion. BMSC‐derived exosomes carrying a RUNX2 promoter‐driven DUSP26 plasmid favor osteogenic‐lineage transgene expression, partially ...
Yunpeng Lv, Feng Wu, Xiongjie Liang, Chong Liu, Wenhui Guo, Kejian Zhang, Jiawen Niu, Wenjie Jiang, Hongjian Xu, Anlong Jiang, Yang Zheng, Enming Yu, Xintao Wang, Jinglong Yan, Jingjun Xia, Chengchao Song   +15 more
wiley   +1 more source

Single molecule imaging reveals differences in microtubule track selection between Kinesin motors.

PLoS Biology, 2009
Cells generate diverse microtubule populations by polymerization of a common alpha/beta-tubulin building block. How microtubule associated proteins translate microtubule heterogeneity into specific cellular functions is not clear.
Dawen Cai, Dyke P McEwen, Jeffery R Martens, Edgar Meyhofer, Kristen J Verhey   +4 more
doaj   +1 more source

A single protofilament is sufficient to support unidirectional walking of dynein and kinesin. [PDF]

PLoS ONE, 2012
Cytoplasmic dynein and kinesin are two-headed microtubule motor proteins that move in opposite directions on microtubules. It is known that kinesin steps by a 'hand-over-hand' mechanism, but it is unclear by which mechanism dynein steps.
Keitaro Shibata, Michi Miura, Yuta Watanabe, Kei Saito, Atsuko Nishimura, Ken'ya Furuta, Yoko Y Toyoshima   +6 more
doaj   +1 more source

Electro-detachment of kinesin motor domain from microtubule in silico

Computational and Structural Biotechnology Journal, 2023
Kinesin is a motor protein essential in cellular functions, such as intracellular transport and cell-division, as well as for enabling nanoscopic transport in bio-nanotechnology.
Jiří Průša, Michal Cifra
doaj   +1 more source

Substrate stiffness regulates cell fate and its applications in the repair of motor system: From two dimensions to three dimensions

BMEMat, EarlyView.
Substrate stiffness with different dimensions (2D and 3D) exerts a regulatory role in cellular behaviors, thereby governing cell fate decisions. Biomaterials with tunable stiffness exhibit substantial functional potential for the repair and regeneration of the motor system.
Zhiyuan Lu, Renwang Sheng, Ludvig J. Backman, Chuanquan Liu, Danning Zheng, Wei Zhang, Jialin Chen   +6 more
wiley   +1 more source

Kinesin-5 Is Dispensable for Bipolar Spindle Formation and Elongation in Candida albicans, but Simultaneous Loss of Kinesin-14 Activity Is Lethal

mSphere, 2019
Mitotic spindles assume a bipolar architecture through the concerted actions of microtubules, motors, and cross-linking proteins. In most eukaryotes, kinesin-5 motors are essential to this process, and cells will fail to form a bipolar spindle without ...
Irsa Shoukat, Corey Frazer, John S. Allingham   +2 more
doaj   +1 more source

Mitochondrial adaptor TRAK2 activates and functionally links opposing kinesin and dynein motors

Nature Communications, 2021
Mitochondria are transported along microtubules by opposing kinesin and dynein motors. Kinesin-1 and dynein-dynactin are linked to mitochondria by TRAK proteins, but it is unclear how TRAKs coordinate these motors. We used single-molecule imaging of cell
Adam R. Fenton, Cecilia R. Petruconis, Thomas A. Jongens, Erika L. F. Holzbaur   +3 more
doaj   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source