Results 81 to 90 of about 47,063 (259)

Heterogeneity in kinesin function [PDF]

Traffic, 2017
The kinesin family proteins are often studied as prototypical molecular motors; a deeper understanding of them can illuminate regulation of intracellular transport. It is typically assumed that they function identically. Here we find that this assumption of homogeneous function appears incorrect: variation among motors’ velocities in vivo and in vitro ...
Reddy, Babu J N, Tripathy, Suvranta, Vershinin, Michael, Tanenbaum, Marvin E, Xu, Jing, Mattson-Hoss, Michelle, Arabi, Karim, Chapman, Dail, Doolin, Tory, Hyeon, Changbong, Gross, Steven P   +10 more
openaire   +3 more sources

Atomic-resolution structure of kinesin-1 motor domain in complex with microtubules by magic angle spinning NMR spectroscopy

, 2022
Polenova, TatyanaMicrotubules (MTs) and their associated proteins play essential roles in maintaining cell structure, organelle transport, cell motility and cell division.
Zhang, Chunting
core   +1 more source

Post‐LECA Origin and Diversification of an Axonemal Outer Arm Dynein Motor

Cytoskeleton, EarlyView.
ABSTRACT Dyneins were present in the last eukaryotic common ancestor (LECA) and play key roles in eukaryotic biology. Axonemal dyneins form the inner and outer arms that power ciliary beating, and it has long been recognized that outer arms in some organisms contain two different heavy chain motors, whereas those from other species contain a third unit
Stephen M. King
wiley   +1 more source

Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast

Nature Communications, 2017
Bipolar spindle assembly requires a balance of kinesin 14 pulling and kinesin 5 pushing forces. Here, the authors show that in fission yeast, spindle formation can occur in the absence of kinesin 5 (Cut7) and 14 (Pkl1) but requires the microtubule ...
Sergio A. Rincon, Adam Lamson, Robert Blackwell, Viktoriya Syrovatkina, Vincent Fraisier, Anne Paoletti, Meredith D. Betterton, Phong T. Tran   +7 more
doaj   +1 more source

Targeted Inactivation of Kinesin-1 in Pancreatic beta-Cells in Vivo Leads to Insulin Secretory Deficiency [PDF]

, 2010
Suppression of Kinesin-1 by antisense oligonucleotides, or overexpression of dominant-negative acting kinesin heavy chain, has been reported to affect the sustained phase of glucose-stimulated insulin secretion in β-cells in vitro.
Cheng, Q, Yao, Kwokming, Nancy A. Jenkins, Wang, Zai, Zhang, XM, Qianni Cheng, Kwok-Ming Yao, Lin, Raozhou, Huang, Jiandong, Copeland, NG, Jenkins, NA, Yao, KM, Jian-Dong Huang, Neal G. Copeland, Huang, JD, Wang, Z, Xin-Mei Zhang, Ju Cui, Raozhou Lin, Lin, R, Po Sing Leung, Cui, J, Leung, Po sing, Zhang, Xinmei, Cui, Ju, Jenkins, Nancy A, Copeland, Neal G, Leung, PS, Cheng, Qianni, Zai Wang   +29 more
core   +1 more source

In Vivo Cytoskeletal AMPA Receptor Transport Imaging in C. elegans

Cytoskeleton, EarlyView.
ABSTRACT Long‐distance intracellular transport of ionotropic glutamate receptors (iGluRs) is essential for proper excitatory synaptic function underlying learning and memory. Many neuropsychiatric and neurodegenerative conditions have abnormal iGluR transport and trafficking, leading to an intense interest in the mechanisms and factors regulating these
Michaelis A. K., Hoerndli F. J.
wiley   +1 more source

Aurora B suppresses microtubule dynamics and limits central spindle size by locally activating KIF4A [PDF]

, 2013
Anaphase central spindle formation is controlled by the microtubule-stabilizing factor PRC1 and the kinesin KIF4A. We show that an MKlp2-dependent pool of Aurora B at the central spindle, rather than global Aurora B activity, regulates KIF4A accumulation
Erich A. Nigg, Francis A. Barr, Baron, Ryan D., Ricardo Nunes Bastos, Nunes Bastos, R, Ryan D. Baron, Nigg, EA, Gruneberg, Ulrike, Gandhi, Sapan R., Baron, RD, Barr, Francis, Gandhi, SR, Nunes Bastos, Ricardo, Ulrike Gruneberg, Nigg, Erich A., Sapan R. Gandhi, Barr, Francis A.   +16 more
core   +1 more source

Kinesin-20 makes a ring-like structure at the base of developing ookinete and is important for zygote to ookinete transformation and subsequent motility.

, 2022
(A) Live cell imaging showing localisation of kinesin-20GFP (green) in the female gametocyte, zygote, and during ookinete transformation. Kinesin-20GFP accumulates at the neck of developing ookinete making a ring-like structure (indicated by arrows ...
Anthony A. Holder (53358), Rita Tewari (134257), Sue Vaughan (20216), Steven Abel (7484636), Zeinab Chahine (13170045), Ravish Rashpa (4278514), Mohammad Zeeshan (141644), Carolyn A. Moores (7484654), Karine G. Le Roch (7373171), David J. P. Ferguson (7484639), Declan Brady (134247), Mathieu Brochet (149272)   +11 more
core   +1 more source

Protocol for Reconstituting Adaptor‐Mediated Activation of Full‐Length Kinesin‐1

Cytoskeleton, EarlyView.
ABSTRACT Kinesin‐1 is a member of the kinesin superfamily that plays an essential role in intracellular cargo transport. In the absence of cargo, Kinesin‐1 exhibits low motor activity due to autoinhibition. Multiple studies have demonstrated that adaptor proteins, which link cargos to Kinesin‐1, can activate Kinesin‐1 by releasing the autoinhibition ...
Haruka Masumoto, Kyoko Chiba
wiley   +1 more source

The conformational cycle of kinesin

Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 2000
The stepping mechanism of kinesin can be thought of as a programme of conformational changes. We briefly review protein chemical, electron microscopic and transient kinetic evidence for conformational changes, and working from this evidence, outline a model for the mechanism. In the model, both kinesin heads initially trap Mg.ADP.
R A, Cross, I, Crevel, N J, Carter, M C, Alonso, K, Hirose, L A, Amos   +5 more
openaire   +3 more sources