Results 131 to 140 of about 5,661 (174)
Some of the next articles are maybe not open access.
Nucleosome organization on Kluyveromyces lactis centromeric DNAs
Biophysical Chemistry, 2002The preferential assembly of specialized nucleosomes on budding yeast centromeres can be due either to the higher stability of specialized centromeric nucleosomes and/or to the lower stability of canonical centromeric nucleosomes with respect to bulk nucleosomes.
Mattei S +3 more
openaire +6 more sources
Expression cloning in Kluyveromyces lactis
Biotechnology Techniques, 1999Kluyveromyces lactis was used as host for an Aspergillus tubingensis expression library. A new episomal vector was constructed to direct the expression of the A. tubingensis cDNAs and to allow subsequent analysis in Escherichia coli. Using three different plate assays, 18000 K.
van der Vlugt-Bergmans, C.J.B. +1 more
openaire +2 more sources
Heme-mediated transcriptional control in Kluyveromyces lactis
Current Genetics, 2000Heme is of great importance in oxygen-dependent biological functions, since it serves as a prosthetic group for many proteins related to oxygen-binding, oxidative damage prevention and electron transport. It also regulates gene expression through the action of specific transcriptional regulatory factors.
M, González-Domínguez +4 more
openaire +2 more sources
Kluyveromyces lactis: An emerging tool in biotechnology
Journal of Biotechnology, 2016Kluyveromyces lactis has emerged as one of the most important yeast species for research and industrial biotechnology. This Crabtree-negative species is suitable for the production of metabolites and heterologous proteins, and its ability to achieve high levels of protein secretion makes it an attractive alternative for industrial protein production ...
Sebastian C, Spohner +3 more
openaire +2 more sources
Synonymous codon usage in Kluyveromyces lactis
Yeast, 1993AbstractThe nature and variation of synonymous codon usage in 47 open reading frames from Kluyveromyces lactis have been investigated. Using multivariate statistical analysis, a single major trend among K. lactis genes was identified that differentiates among genes by expression level: highly expressed genes have high codon usage bias, while genes of ...
A T, Lloyd, P M, Sharp
openaire +2 more sources
Properties of mitochondrial DNA from Kluyveromyces lactis
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974Abstract 1. We have isolated a closed circular DNA fraction from mitochondria purified from Kluyveromyces lactis by centrifuging a mitochondrial lysate to equilibrium in CsCl containing ethidium bromide. Electron micrographs of appropriate gradient fractions show predominantly circular duplex DNA with an average contour length ( ± S.D.
J P, Sanders +3 more
openaire +2 more sources
Transformation of Kluyveromyces lactis
2003Often the aim of a transformation is to obtain at least one transformant that can be used in further experiments. This transformant should contain either a linear fragment integrated at the correct position in the genome or a plasmid. On the other hand, for transformation with a plasmid library, for example, high transformation efficiency is required ...
Rolf A. Kooistra, H. Yde Steensma
openaire +1 more source
Efficient gene targeting in Kluyveromyces lactis
Yeast, 2004AbstractIntegration of a DNA fragment in a host genome requires the action of a double‐strand break (DSB) repair mechanism. Homologous recombination (HR) is initiated by binding of Rad52p to DNA ends and results in targeted integration. Binding of the Ku heterodimer (Ku70p/Ku80p) results in random integration via non‐homologous end joining (NHEJ).
Rolf, Kooistra +2 more
openaire +2 more sources
Finding of dairy yeasts Kluyveromyces lactis var. lactis in natural habitats
Microbiology, 2014Well-known yeasts Kluyveromyces lactis var. lactis, which are usually associated with dairy prod- ucts, were discovered in nature (in woodland park soil under Impatiens glandulifera Royle plants). Reliable identification of the yeasts was carried out using physiological criteria (lactose and maltose utilization) and molecular markers (nucleotide ...
G I, Naumov +4 more
openaire +2 more sources
Molecular biology of Kluyveromyces lactis
1993The yeast Saccharomyces cerevisiae is probably the most thoroughly understood amongst eukaryotic organisms and an excellent model for the study of eukaryotic cells in general; indeed, the term “yeast” is often used as a synonim for this species. Recently, however, other yeasts have attracted the attention of researchers as a result of their distinct ...
FRONTALI, Laura +7 more
openaire +2 more sources