Results 1 to 10 of about 9,062 (102)

Isolation of antigen-specific, disulphide-rich knob domain peptides from bovine antibodies. [PDF]

PLoS Biology, 2020
As a novel alternative to established surface display or combinatorial chemistry approaches for the discovery of therapeutic peptides, we present a method for the isolation of small, cysteine-rich domains from bovine antibody ultralong complementarity ...
Alex Macpherson, Anthony Scott-Tucker, Anastasios Spiliotopoulos, Catherine Simpson, Justin Staniforth, Adam Hold, James Snowden, Leah Manning, Jean van den Elsen, Alastair D G Lawson   +9 more
doaj   +3 more sources

A novel subunit vaccine based on Fiber1/2 knob domain provides full protection against fowl adenovirus serotype 4 and induces stronger immune responses than a Fiber2 subunit vaccine [PDF]

Poultry Science
: Outbreaks of hepatitis-hydropericardium syndrome (HHS) caused by fowl adenovirus serotype 4 (FAdV-4) have resulted in huge economic losses to the poultry industry in China since 2015.
Shuaifeng Liu, Xiaofeng Dong, Baishi Lei, Wuchao Zhang, Xiangqin Wang, Wanzhe Yuan, Kuan Zhao   +6 more
doaj   +4 more sources

The allosteric modulation of complement C5 by knob domain peptides [PDF]

eLife, 2021
Bovines have evolved a subset of antibodies with ultra-long heavy chain complementarity determining regions that harbour cysteine-rich knob domains. To produce high-affinity peptides, we previously isolated autonomous 3–6 kDa knob domains from bovine ...
Alex Macpherson, Maisem Laabei, Zainab Ahdash, Melissa A Graewert, James R Birtley, Monika-Sarah ED Schulze, Susan Crennell, Sarah A Robinson, Ben Holmes, Vladas Oleinikovas, Per H Nilsson, James Snowden, Victoria Ellis, Tom Eirik Mollnes, Charlotte M Deane, Dmitri Svergun, Alastair DG Lawson, Jean MH van den Elsen   +17 more
doaj   +2 more sources

Serum albumin binding knob domains engineered within a VH framework III bispecific antibody format and as chimeric peptides

Frontiers in Immunology, 2023
BackgroundSerum albumin binding is an established mechanism to extend the serum half-life of antibody fragments and peptides. The cysteine rich knob domains, isolated from bovine antibody ultralong CDRH3, are the smallest single chain antibody fragments ...
Ralph Adams, Callum Joyce, Mikhail Kuravskiy, Katriona Harrison, Katriona Harrison, Zainab Ahdash, Matthew Balmforth, Kelda Chia, Cinzia Marceddu, Matthew Coates, James Snowden, Emmanuel Goursaud, Karelle Ménochet, Jean van den Elsen, Richard J. Payne, Richard J. Payne, Alastair D. G. Lawson, Anthony Scott-Tucker, Alex Macpherson   +18 more
doaj   +3 more sources

Precise location of three novel linear epitopes using the generated monoclonal antibodies against the Knob domain of FAdV-4 surface structural protein, fiber1 [PDF]

Frontiers in Cellular and Infection Microbiology
BackgroundFowl adenovirus serotype 4 (FAdV-4) is the main pathogen of hepatitis-hydropericardium syndrome (HHS), which brings huge economic losses to the poultry industry worldwide.
Yongxiao Chai, Yongxiao Chai, Qianyue Jin, Rongfang Zhu, Zhenhua Guo, Qingxia Lu, Shujun Chai, Yunrui Xing, Lu Han, Guangxu Xing, Gaiping Zhang, Gaiping Zhang, Gaiping Zhang   +12 more
doaj   +2 more sources

Modular design of bi- and multi-specific knob domain fusions [PDF]

Frontiers in Immunology
IntroductionThe therapeutic potential of bispecific antibodies is becoming widely recognised, with over a hundred formats already described. For many applications, enhanced tissue penetration is sought, so bispecifics with low molecular weight may offer ...
Mikhail Kuravsky, Glyn F. Gibbons, Callum Joyce, Anthony Scott-Tucker, Alex Macpherson, Alastair D. G. Lawson   +5 more
doaj   +2 more sources

The proximity of the N- and C- termini of bovine knob domains enable engineering of target specificity into polypeptide chains

mAbs, 2022
Cysteine-rich knob domains can be isolated from the ultralong heavy-chain complementarity-determining region (CDR) 3, which are unique to a subset of bovine antibodies, to create antibody fragments of ~4 kDa.
Alice Hawkins, Callum Joyce, Kevin Brady, Adam Hold, Alan Smith, Michael Knight, Conor Howard, Jean van den Elsen, Alastair D.G. Lawson, Alex Macpherson   +9 more
doaj   +3 more sources

Comparative Molecular Dynamics Study of 19 Bovine Antibodies with Ultralong CDR H3 [PDF]

Antibodies
Background/Objectives: Cows produce antibodies with ultralong CDRH3 segments (ulCABs) that contain a disulfide-stabilized knob domain. This domain is connected to the globular core of the antibody by a β-strand stalk. In the crystal structures, the stalk
Olena Denysenko, Anselm H. C. Horn, Heinrich Sticht   +2 more
doaj   +2 more sources

The Knob Domain of the Fiber-1 Protein Affects the Replication of Fowl Adenovirus Serotype 4 [PDF]

Microorganisms
Fowl adenovirus serotype 4 (FAdV-4) outbreaks have caused significant economic losses in the Chinese poultry industry since 2015. The relationships among viral structural proteins in infected hosts are relatively unknown. To explore the role of different
Xiaofeng Li, Zhixun Xie, You Wei, Zhiqin Xie, Aiqiong Wu, Sisi Luo, Liji Xie, Meng Li, Yanfang Zhang   +8 more
doaj   +2 more sources

Insights into the molecular determinants of host specificity in Pseudomonas aeruginosa-infecting phages: a structural and functional analysis of tail fibre proteins [PDF]

BMC Biology
Background Bacteriophages and bacteria frequently occupy the same ecological niches, driving complex and dynamic host–virus interactions. In Pseudomonas aeruginosa, phages from the Migulavirinae subfamily, tail fibre proteins (TFPs) are crucial to host ...
Aleksandra Zalewska, Agata Jurczak-Kurek, Magdalena Kwiatek, Heejoon Myung, Marcin Górniak   +4 more
doaj   +2 more sources