Design and cellular internalization of genetically engineered polypeptide nanoparticles displaying adenovirus knob domain [PDF]
Journal of Controlled Release, 2011 Hepatocytes and acinar cells exhibit high-efficiency, fiber-dependent internalization of adenovirus; however, viral capsids have unpredictable immunological effects and are challenging to develop into targeted drug carriers. To exploit this internalization pathway and minimize the use of viral proteins, we developed a simple gene product that self ...
Sarah F Hamm-Alvarez, J Andrew Mackay
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The Chemical Synthesis of Knob Domain Antibody Fragments [PDF]
ACS Chemical Biology, 2021 Cysteine-rich knob domains found in the ultralong complementarity determining regions of a subset of bovine antibodies are capable of functioning autonomously as 3-6 kDa peptides. While they can be expressed recombinantly in cellular systems, in this paper we show that knob domains are also readily amenable to a chemical synthesis, with a co-crystal ...
Alex Macpherson +2 more
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Comparative Molecular Dynamics Study of 19 Bovine Antibodies with Ultralong CDR H3 [PDF]
AntibodiesBackground/Objectives: Cows produce antibodies with ultralong CDRH3 segments (ulCABs) that contain a disulfide-stabilized knob domain. This domain is connected to the globular core of the antibody by a β-strand stalk. In the crystal structures, the stalk
Olena Denysenko +2 more
doaj +2 more sources
Insights into the molecular determinants of host specificity in Pseudomonas aeruginosa-infecting phages: a structural and functional analysis of tail fibre proteins [PDF]
BMC BiologyBackground Bacteriophages and bacteria frequently occupy the same ecological niches, driving complex and dynamic host–virus interactions. In Pseudomonas aeruginosa, phages from the Migulavirinae subfamily, tail fibre proteins (TFPs) are crucial to host ...
Aleksandra Zalewska +4 more
doaj +2 more sources
Characterization of the knob domain of the adenovirus type 5 fiber protein expressed in Escherichia coli [PDF]
Journal of Virology, 1994 The adenovirus fiber protein is used for attachment of the virus to a specific receptor on the cell surface. Structurally, the protein consists of a long, thin shaft that protrudes from the vertex of the virus capsid and terminates in a globular domain termed the knob. To verify that the knob is the domain which interacts with the cellular receptor, we
Johann Deisenhofer, R D Gérard
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Incorporation of Peptides Targeting EGFR and FGFR1 into the Adenoviral Fiber Knob Domain and Their Evaluation as Targeted Cancer Therapies. [PDF]
Hum Gene Ther, 2015 Oncolytic virotherapies based on adenovirus 5 (Ad5) hold promise as adjunctive cancer therapies; however, their efficacy when delivered systemically is hampered by poor target cell specificity and preexisting anti-Ad5 immunity. Ovarian cancer represents a promising target for virotherapy, since the virus can be delivered locally ...
Uusi-Kerttula H +8 more
europepmc +4 more sources
The Maturation of Murine Dendritic Cells Induced by Human Adenovirus Is Mediated by the Fiber Knob Domain [PDF]
Journal of Biological Chemistry, 2003 We investigated the mechanism of adenovirus serotype 5 (Ad5)-mediated maturation of bone marrow-derived murine dendritic cells (DC) using (i) Ad5 vectors with wild-type capsid (AdE1 degrees, AdGFP); (ii) Ad5 vector mutant deleted of the fiber C-terminal knob domain (AdGFPDeltaknob); and (iii) capsid components isolated from Ad5-infected cells or ...
Valerie Molinier-Frenkel +2 more
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Generation of a rabbit VH domain antibody polyspecific to c-Met and adenoviral knob protein
Biochemical and Biophysical Research Communications, 2006 Several types of bispecific antibodies with affinity to both adenoviral coat proteins and a targeted antigen have been developed with the aim of providing the specific delivery of adenoviral gene therapy vehicle. From a phage display library of combinatorial dAb2s (each with an anti-adenoviral knob protein V(H) fragment linked with an anti-c-Met V(H)),
Ki Su Kim, Chae-Ok Yun, Junho Chung
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Adenovirus type 5 fiber knob binds to MHC class I α2 domain at the surface of human epithelial and B lymphoblastoid cells [PDF]
EMBO Journal, 1997 Adenovirus serotype 5 (Ad5) fiber receptor was investigated using reverse antibody biopanning of a phage-displayed hexapeptide library, and virus-neutralizing monoclonal antibodies (mAbs 1D6.3 and 7A2.7) raised against recombinant Ad5 fiber knob. Both mAbs inhibited attachment of Ad5 to HeLa cells. Mimotopes of 1D6.3 showed homology with the C-terminal
Saw See Hong, David T Curiel
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Journal of Virology, 2020 Among 12 serotypes of fowl adenovirus (FAdV), FAdV-1, FAdV-4, and FAdV-10 all carry two fiber genes (i.e., fiber-1 and fiber-2), whereas other serotypes have only one. As important viral surface proteins, the fibers play vital roles in the infection and pathogenesis of FAdV.
Tuofan Li, Hongjun Chen, Quan Xie
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