Lac Operon Boolean Models: Dynamical Robustness and Alternative Improvements
Mathematics, 2021 In Veliz-Cuba and Stigler 2011, Boolean models were proposed for the lac operon in Escherichia coli capable of reproducing the operon being OFF, ON and bistable for three (low, medium and high) and two (low and high) parameters, representing the ...
Thomas Ledger, Gonzalo A Ruz, Eric Goles
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Wrapped-around models for the lac operon complex. [PDF]
Biophys J, 2010 The protein-DNA complex, involved in the lac operon of enteric bacteria, is paradigmatic in understanding the extent of DNA bending and plasticity due to interactions with protein assemblies acting as DNA regulators. For the lac operon, two classes of structures have been proposed: 1), with the protein tetramer lying away from the DNA loop (wrapped ...
La Penna G, Perico A.
europepmc +6 more sources
Deterministic and stochastic population-level simulations of an artificial
BMC Bioinformatics, 2011 Background The lac operon genetic switch is considered as a paradigm of genetic regulation. This system has a positive feedback loop due to the LacY permease boosting its own production by the facilitated transport of inducer into the cell and the ...
Zygourakis Kyriacos, Stamatakis Michail
doaj +2 more sources
Bistable behavior of the lac operon in E. coli when induced with a mixture of lactose and TMG [PDF]
Frontiers in Physiology, 2010 In this work we investigate multistability in the lac operon of Escherichia coli when it is induced by a mixture of lactose and the non-metabolizable thiomethyl galactoside (TMG).
Orlando Díaz-Hernández +3 more
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Interplay of protein and DNA structure revealed in simulations of the lac operon. [PDF]
PLoS ONE, 2013 The E. coli Lac repressor is the classic textbook example of a protein that attaches to widely spaced sites along a genome and forces the intervening DNA into a loop.
Luke Czapla +3 more
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Genomic characterization of Klebsiella spp. from bovine mastitis: dissemination of a conserved, highly transmissible lacacq+ fec+ plasmid drives burden of disease [PDF]
Applied and Environmental MicrobiologyBovine mastitis poses a significant health concern for dairy cattle and a major economic burden on the dairy industry. Klebsiella spp. are important mastitis pathogens, with previous studies identifying lactose-utilization (lacacq) and iron-acquisition ...
Michael Biggel +5 more
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Identification of an intestine-specific promoter and inducible expression of bacterial α-galactosidase in mammalian cells by a
Journal of Animal Science and Biotechnology, 2012 Background α-galactosidase has been widely used in animal husbandry to reduce anti-nutritional factors (such as α-galactoside) in feed. Intestine-specific and substrate inducible expression of α-galactosidase would be highly beneficial for transgenic ...
Ya-Feng Zhai +8 more
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Expanding the Toolbox for Inducible Protein Expression With Automation-enabled Generation of Glycomimetics. [PDF]
ChemistryInducible protein expression is a cornerstone of many aspects of industrial and molecular biotechnological processes. 2′‐fucosyl isopropyl‐β‐D‐thiogalactopyranose (2′F‐IPTG) and 2′‐fucosyl isobutyl‐C‐galactoside (2′F‐IBCG) mimics were explored for protein expression studies.
DeYong AE +6 more
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PLoS ONE, 2021 The cAMP-CRP regulon coordinates transcription regulation of several energy-related genes, the lac operon among them. Lactose, or IPTG, induces the lac operon expression by binding to the LacI repressor, and releasing it from the promoter sequence.
Chunghwan Ro +2 more
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Frontiers in Microbiology, 2022 Lacticaseibacillus casei is used extensively in the fermented milk-beverage industry as a starter culture. Acid production capacity during fermentation is the main criterion for evaluating starters although it is strain-dependent. In this study, the acid
Xiaoxia Li +11 more
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