Adaptive evolution of the lactose utilization network in experimentally evolved populations of Escherichia coli. [PDF]
PLoS Genetics, 2012 Adaptation to novel environments is often associated with changes in gene regulation. Nevertheless, few studies have been able both to identify the genetic basis of changes in regulation and to demonstrate why these changes are beneficial.
Selwyn Quan +6 more
doaj +1 more source
Expanding the Toolbox for Inducible Protein Expression With Automation-enabled Generation of Glycomimetics. [PDF]
ChemistryInducible protein expression is a cornerstone of many aspects of industrial and molecular biotechnological processes. 2′‐fucosyl isopropyl‐β‐D‐thiogalactopyranose (2′F‐IPTG) and 2′‐fucosyl isobutyl‐C‐galactoside (2′F‐IBCG) mimics were explored for protein expression studies.
DeYong AE +6 more
europepmc +2 more sources
Delineating the Role of the msaABCR Operon in Staphylococcal Overflow Metabolism
Frontiers in Microbiology, 2022 Staphylococcus aureus is an important human pathogen that can infect almost every organ system, resulting in a high incidence of morbidity and mortality. The msaABCR operon is an important regulator of several staphylococcal phenotypes, including biofilm
Bibek G C +2 more
doaj +1 more source
Nonlinear fitness landscape of a molecular pathway. [PDF]
PLoS Genetics, 2011 Genes are regulated because their expression involves a fitness cost to the organism. The production of proteins by transcription and translation is a well-known cost factor, but the enzymatic activity of the proteins produced can also reduce fitness ...
Lilia Perfeito +4 more
doaj +1 more source
Severe Restriction of Glucose Import Enhances Recombinant Protein Production in Escherichia coli. [PDF]
Biotechnol JGraphical Abstract and Lay Summary Producing recombinant proteins in Escherichia coli is often limited by the bacterium's tendency to consume glucose too rapidly. This excessive glucose uptake causes overflow metabolism, leading to the accumulation of acetate, a byproduct that can reduce cellular performance and protein production.
Fragoso-Jiménez JC +5 more
europepmc +2 more sources
Activation of Silent gal Genes in the lac-gal Regulon of Streptococcus thermophilus [PDF]
, 2001 Streptococcus thermophilus strain CNRZ 302 is unable to ferment galactose, neither that generated intracellularly by lactose hydrolysis nor the free sugar.
Vos, W.M., de +17 more
core +1 more source
BMC Microbiology, 2020 Background The msaABCR operon regulates several staphylococcal phenotypes such as biofilm formation, capsule production, protease production, pigmentation, antibiotic resistance, and persister cells formation.
Gyan S. Sahukhal +4 more
doaj +1 more source
Supercoiling Effects on Short-Range DNA Looping in E. coli. [PDF]
PLoS ONE, 2016 DNA-protein loops can be essential for gene regulation. The Escherichia coli lactose (lac) operon is controlled by DNA-protein loops that have been studied for decades.
Lauren S Mogil +2 more
doaj +1 more source
The Inducible lac Operator-Repressor System Is Functional in Zebrafish Cells
Frontiers in Genetics, 2021 BackgroundZebrafish are a foundational model organism for studying the spatio-temporal activity of genes and their regulatory sequences. A variety of approaches are currently available for editing genes and modifying gene expression in zebrafish ...
Sierra S. Nishizaki +8 more
doaj +1 more source
Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing. [PDF]
PLoS ONE, 2020 The combination of a fluorescent reporter and enzymatic reporter provides a flexible and versatile way for the study of diverse biological processes, such as the detection of transcription and translation.
Chang-Ye Hui +5 more
doaj +1 more source