Results 31 to 40 of about 36,597 (195)

DNA looping: the consequences and its control [PDF]

, 2006
The formation of DNA loops by proteins and protein complexes is ubiquitous to many fundamental cellular processes, including transcription, recombination, and replication.
Ackers, Adhya, Alberts, Becker, Bloomfield, Broach, Cloutier, de Lange, Dodd, Du, Dunn, Halford, Hanke, Hill, Irani, Jose MG Vilar, Knight, Leonor Saiz, Lewis, Li, Matthews, Moreau, Mossing, Muller, Müller-Hill, Oehler, Olson, Ptashne, Ptashne, Radman-Livaja, Revet, Richmond, Ringrose, Rippe, Saiz, Saiz, Schleif, Semsey, Stenger, Tolhuis, Vilar, Vilar, Villa, Yan, Yasmin, Zhang   +45 more
core   +1 more source

Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing. [PDF]

PLoS ONE, 2020
The combination of a fluorescent reporter and enzymatic reporter provides a flexible and versatile way for the study of diverse biological processes, such as the detection of transcription and translation.
Chang-Ye Hui, Yan Guo, Lisa Liu, Hao-Qu Zheng, Chao-Xian Gao, Wen Zhang   +5 more
doaj   +1 more source

Physiological regulation of a decontrolled lac operon [PDF]

Journal of Bacteriology, 1977
The expression of the lac operon was studied under a variety of growth conditions in induced and in constitutive cells of Escherichia coli that carried different catabolite-insensitive lac promoters. Use of such "decontrolled" lac operons permitted a study of the expression of an operon that was presumably subject only to passive control. Since the use
B L, Wanner, R, Kodaira, F C, Neidhardt
openaire   +2 more sources

Concentration and Length Dependence of DNA Looping in Transcriptional Regulation [PDF]

, 2008
In many cases, transcriptional regulation involves the binding of transcription factors at sites on the DNA that are not immediately adjacent to the promoter of interest.
Beausang, John F., Blumberg, Seth, Garcia, Hernan G., Han, Lin, Nelson, Philip C., Phillips, Rob, Towles, Kevin B.   +6 more
core   +7 more sources

Development of a Positive-readout Mouse Model of siRNA Pharmacodynamics

Molecular Therapy: Nucleic Acids, 2013
Development of RNAi-based therapeutics has the potential to revolutionize treatment options for a range of human diseases. However, as with gene therapy, a major barrier to progress is the lack of methods to achieve and measure efficient delivery for ...
Mark Stevenson, Robert Carlisle, Ben Davies, Chris Preece, Michelle Hammett, Wei-li Liu, Kerry David Fisher, Amy Ryan, Heidi Scrable, Leonard William Seymour   +9 more
doaj   +1 more source

GlaR (YugA)—a novel RpiR‐family transcription activator of the Leloir pathway of galactose utilization in Lactococcus lactis IL1403

MicrobiologyOpen, 2019
Bacteria can utilize diverse sugars as carbon and energy source, but the regulatory mechanisms directing the choice of the preferred substrate are often poorly understood.
Tamara Aleksandrzak‐Piekarczyk, Katarzyna Szatraj, Katarzyna Kosiorek   +2 more
doaj   +1 more source

A modified consumer inkjet for spatiotemporal control of gene expression.

PLoS ONE, 2009
This paper presents a low-cost inkjet dosing system capable of continuous, two-dimensional spatiotemporal regulation of gene expression via delivery of diffusible regulators to a custom-mounted gel culture of E. coli. A consumer-grade, inkjet printer was
Daniel J Cohen, Roberto C Morfino, Michel M Maharbiz   +2 more
doaj   +1 more source

In silico approach for the identification of mirror repeats in selected operon genes of Escherichia coli strain K-12 substrain MG1655

Biomedical and Biotechnology Research Journal, 2022
Background: The repeating elements in the genes or genomes of living organisms are associated with a variety of functions at the molecular level. Mirror repeats (MRs) are unique type of repeat sequences among them, which are found to be linked with H-DNA
Sandeep Yadav, Usha Yadav, Dinesh Chandra Sharma   +2 more
doaj   +1 more source

Regulation of bistability in the std fimbrial operon of Salmonella enterica by DNA adenine methylation and transcription factors HdfR, StdE and StdF [PDF]

, 2019
Bistable expression of the Salmonella enterica std operon is controlled by an AND logic gate involving three transcriptional activators: the LysR-type factor HdfR and the StdE and StdF regulators encoded by the std operon itself.
Casadesús Pursals, Josep, García Pastor, Lucía, Jakomin, Marcello, Puerta Fernández, Elena, Sánchez Romero, María Antonia   +4 more
core   +1 more source

Direct measurements of IPTG enable analysis of the induction behavior of E. coli in high cell density cultures

Microbial Cell Factories, 2012
Background The E. coli lac operon and its components have been studied for decades, and lac-derived systems are widely used for recombinant protein production.
Fernández-Castané Alfred, Caminal Glòria, López-Santín Josep   +2 more
doaj   +1 more source