Results 41 to 50 of about 21,116 (197)
The Role of Integration Host Factor in Escherichia coli Persister Formation
mBio, 2022 Persisters represent a small subpopulation of cells that are tolerant of killing by antibiotics and are implicated in the recalcitrance of chronic infections to antibiotic therapy.
Samantha E. Nicolau, Kim Lewis
doaj +1 more source
Understanding operon architecture using LEGO bricks
Journal of Microbiology & Biology EducationMany undergraduates struggle to interpret abstract concepts in molecular biology. Modeling can facilitate learning by making these abstract concepts tangible.
Dena R. Hammond-Weinberger +1 more
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Tools for characterization of Escherichia coli genes of unknown function
, 2002 Despite the power of sequencing and of emerging high-throughput technologies to collect data rapidly, the definitive functional characterization of unknown genes still requires biochemical and genetic analysis in case-by-case studies. This often involves
Masters, Millicent +3 more
core +1 more source
Transcription of the lac Operon of Escherichia coli
Journal of Biological Chemistry, 1974 The effects of induction and of transient and catabolite repression on lac-specific RNA in cells of Escherichia coli were investigated. The rate of lac-specific RNA synthesis increases linearly for 3 min after induction and then becomes constant. The accumulation of RNA complementary to the promoter proximal lac Z gene begins before accumulation of RNA
T G, Cooper, B, Magasanik
openaire +2 more sources
Relatively slow stochastic gene-state switching in the presence of positive feedback significantly broadens the region of bimodality through stabilizing the uninduced phenotypic state. [PDF]
PLoS Computational Biology, 2018 Within an isogenic population, even in the same extracellular environment, individual cells can exhibit various phenotypic states. The exact role of stochastic gene-state switching regulating the transition among these phenotypic states in a single cell ...
Hao Ge +3 more
doaj +1 more source
Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity
Advanced Science, EarlyView.This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley +1 more source
Characterization of lac operon regulation.
, 2012 A) Examples of the range of evolved LacZ activity phenotypes present in the four evolution environments. Degree of blue coloration on TGX plates gives a qualitative measure of LacZ activity.
Tim F. Cooper (181685) +6 more
core +1 more source
LacSwitch® II Regulation of Connexin43 cDNA Expression Enables Gap-Junction Single-Channel Analysis
BioTechniques, 2003 Metabolic and electrical coupling through gap junction channels is implicated in cell differentiation, tissue homeostasis, and electrotonic propagation of signals in excitable tissues.
G. Zhong +6 more
doaj +1 more source
, 2023 The lac operon is one of the best known gene regulatory circuits and constitutes a landmark example of how bacteria tune their metabolism to nutritional conditions.
Ana Sousa (261625) +3 more
core +1 more source
Molecular Microbiology, EarlyView.A chromosomal mutation allows bypass of deletion of the Escherichia coli fabH gene. The bypass results from creation of a new promoter that drives increased madA transcription, resulting in increased synthesis of acetyl‐acyl carrier protein by decarboxylation of malonyl‐acyl carrier protein.
Qiaoqiao Guo +5 more
wiley +1 more source