Results 71 to 80 of about 128,431 (240)

In Vivo Biosynthesis and Direct Incorporation of Non‐Canonical Amino Acids into Proteins

ChemBioChem, Accepted Article.
Autonomous cells are engineered biological systems capable of biosynthesising and directly incorporating non‐canonical amino acids (ncAAs) into proteins. These systems have the potential to extend the applicability of the genetic code to enable large‐scale fermentative production of proteins carrying ncAAs.
Ivana Drienovska, Jan Hendrik Illies, T. Moritz Weber   +2 more
wiley   +1 more source

Regulation of endothelial-specific transgene expression by the LacI repressor protein in vivo. [PDF]

PLoS ONE, 2014
Genetically modified mice have played an important part in elucidating gene function in vivo. However, conclusions from transgenic studies may be compromised by complications arising from the site of transgene integration into the genome and, in ...
Susan K Morton, Daniel J Chaston, Brett K Baillie, Caryl E Hill, Klaus I Matthaei   +4 more
doaj   +1 more source

Kinetic approaches to lactose operon induction and bimodality

, 2013
The quasi-equilibrium approximation is acceptable when molecular interactions are fast enough compared to circuit dynamics, but is no longer allowed when cellular activities are governed by rare events.
Michel, Denis
core   +3 more sources

The lac operon galactoside acetyltransferase

Comptes Rendus. Biologies, 2005
Of the proteins encoded by the three structural genes of the lac operon, the galactoside acetyltransferase (thiogalactoside transacetylase, LacA, GAT) encoded by lacA is the only protein whose biological role remains in doubt. Here, we briefly note the classical literature that led to the identification and initial characterization of GAT, and focus on
openaire   +3 more sources

Origin and characterization of cyclodepsipeptides: Comprehensive structural approaches with focus on mass spectrometry analysis of alkali‐cationized molecular species

Mass Spectrometry Reviews, EarlyView.
Abstract Cyclodepsipeptides (CDPs) represent a huge family of chemically and structurally diverse molecules with a wide ability for molecular interactions. CDPs are cyclic peptide‐related natural products made up of both proteinogenic and nonproteinogenic amino acids linked by amide and ester bonds.
Sophie Liuu, Annelaure Damont, Alain Perret, Olivier Firmesse, François Becher, Gwenaëlle Lavison‐Bompard, Amandine Hueber, Amina S. Woods, Ekaterina Darii, François Fenaille, Jean‐Claude Tabet   +10 more
wiley   +1 more source

The impact of metabolism on the adaptation of organisms to environmental change

Frontiers in Cell and Developmental Biology, 2023
Since Jacob and Monod’s discovery of the lac operon ∼1960, the explanations offered for most metabolic adaptations have been genetic. The focus has been on the adaptive changes in gene expression that occur, which are often referred to as “metabolic ...
Douglas L. Rothman, Douglas L. Rothman, Douglas L. Rothman, Peter B. Moore, Peter B. Moore, Robert G. Shulman, Robert G. Shulman   +6 more
doaj   +1 more source

Dual mechanism of repression at a distance in the lac operon. [PDF]

Proceedings of the National Academy of Sciences, 1988
The mechanism by which the internal lacZ gene sequence O2 influences lac repression was investigated by using in vivo footprinting of operon mutants. Quantitative in vivo binding curves show that O2 strengthens by approximately 3-fold repressor binding to O1 that is located 400 base pairs upstream at the transcription start site.
Yehuda Flashner, Jay D. Gralla
openaire   +3 more sources

Staphylococcus aureus COL: An Atypical Model Strain of MRSA That Exhibits Slow Growth and Antibiotic Tolerance due to a Mutation in PRPP Synthetase

Molecular Microbiology, EarlyView.
COL is an unusual “model” strain of Staphylococcus aureus that exhibits slow growth and multidrug antibiotic tolerance. This phenotype is primarily due to a mutation in Prs, which synthesizes the core metabolite phosphoribosyl pyrophosphate (PRPP). Introduction of the COL Prs allele into the antibiotic‐susceptible strain Newman confers tolerance, while
Claire E. Stevens, Ashley T. Deventer, Paul R. Johnston, Phillip T. Lowe, Alisdair B. Boraston, Joanne K. Hobbs   +5 more
wiley   +1 more source

Transcriptional downregulation of agr expression in Staphylococcus aureus during growth in human serum can be overcome by constitutively active mutant forms of the sensor kinase AgrC [PDF]

, 2013
The temporal and cell density-dependent regulation of expression of virtually all the Staphylococcus aureus virulon is under the control of the agr (accessory gene regulatory) operon.
Edwards, AM, James, EH, Wigneshweraraj, S   +2 more
core   +1 more source

Beyond conservation: the landscape of chloroplast genome rearrangements in angiosperms

New Phytologist, EarlyView.
Summary Chloroplast genomes (plastomes) have long been considered structurally conserved, but recent sequencing efforts have uncovered pervasive rearrangements that challenge this assumption. This review catalogues the main types of plastome modifications: large and small inversions; insertions and deletions (indels); gene and intron losses; horizontal
Luiz Augusto Cauz‐Santos
wiley   +1 more source