Results 101 to 110 of about 20,144 (147)

Lysozyme expression in Lactococcus lactis

Applied Microbiology and Biotechnology, 1992
Three lysozyme-encoding genes, one of eukaryotic and two of prokaryotic origin, were expressed in Lactococcus lactis subsp. lactis. Hen egg white lysozyme (HEL) could be detected in L. lactis lysates by Western blotting. No lysozyme activity was observed, however, presumably because of the absence of correctly formed disulphide bonds in the L.
Guchte, Maarten van de, Wal, Fimme Jan van der, Kok, Jan, Venema, Gerhardus   +3 more
openaire   +2 more sources

Genome plasticity in Lactococcus lactis

Antonie van Leeuwenhoek, 2002
Comparative genome analyses contribute significantly to our understanding of bacterial evolution and indicate that bacterial genomes are constantly evolving structures. The gene content and organisation of chromosomes of lactic acid bacteria probably result from a strong evolutionary pressure toward optimal growth of these microorganisms in milk.
Nathalie, Campo, Miguel J, Dias, Marie-Line, Daveran-Mingot, Paul, Ritzenthaler, Pascal, Le Bourgeois   +4 more
openaire   +2 more sources

Autolysis of Lactococcus lactis

International Dairy Journal, 2002
Abstract During cheese making, autolysis of Lactococcus lactis starter bacteria affects cheese flavour development through release of intracellular enzymes. The gene for the major autolysin in L. lactis, N-acetyl muramidase (AcmA), has been cloned and sequenced. The activity of AcmA alone, however, does not explain the huge variation in the extent of
Christopher J. Pillidge, Prasad S.V.S. Rallabhandi, Xing-Zhang Tong, Pramod K. Gopal, Peter C. Farley, Patrick A. Sullivan   +5 more
openaire   +1 more source

Restriction-modification systems in lactococcus lactis

Gene, 1995
Several restriction-modification (R-M) systems have been identified in Lactococcus lactis. Most of the systems have been plasmid encoded and function as phage-resistance mechanisms. At least five different type-II R-M systems, LlaAI, LlaBI, LlaCI, LlaDI and LlaEI, were identified in isolates from a mixed Cheddar starter culture.
N, Nyengaard, F K, Vogensen, J, Josephsen   +2 more
openaire   +2 more sources

Using Lactococcus lactis for glutathione overproduction

Applied Microbiology and Biotechnology, 2004
Glutathione and gamma-glutamylcysteine were produced in Lactococcus lactis using a controlled expression system and the genes gshA and gshB from Escherichia coli encoding the enzymes gamma-glutamylcysteine synthetase and glutathione synthetase. High levels of gamma-glutamylcysteine were found in strains growing on chemically defined medium and ...
Li, Y., Hugenholtz, J., Sybesma, W., Abee, T., Molenaar, D.   +4 more
openaire   +3 more sources

Enhancement of Nisin Production by Lactococcus lactis subsp. lactis

Probiotics and Antimicrobial Proteins, 2016
Lactococcus lactis subsp lactis BSA (L. lactis BSA) was isolated from a commercial fermented product (BSA Food Ingredients, Montreal, Canada) containing mixed bacteria that are used as starter for food fermentation. In order to increase the bacteriocin production by L. lactis BSA, different fermentation conditions were conducted.
Dussault, Dominic, Vu, Khanh Dang, Lacroix, Monique   +2 more
openaire   +3 more sources

Electrotransformation of Lactococcus lactis

2000
The last decade has seen a spectacular increase in genetic technology of Lactococcus lactis, the model lactic acid bacterium extensively used as starter culture in the manufacture of dairy products. The development of transformation techniques (Gasson & Fitzgerald 1994) and construction of powerful plasmids for gene cloning (de Vos & Simons 1994), as ...
Pascal Le Bourgeois, Philippe Langella, Paul Ritzenthaler   +2 more
openaire   +1 more source

Characterization of N-deoxyribosyltransferase from Lactococcus lactis subsp. lactis

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2007
A nucleoside N-deoxyribosyltransferase-homologous gene was detected by homological search in the genomic DNA of Lactococcus lactis subsp. lactis. The gene yejD is composed of 477 nucleotides encoding 159 amino acids with only 25% identity, which is low in comparison to the amino acid sequences of the N-deoxyribosyltransferases from other lactic acid ...
Yukiko, Miyamoto, Takeharu, Masaki, Shigeru, Chohnan   +2 more
openaire   +2 more sources

Protein export elements from Lactococcus lactis

Molecular and General Genetics MGG, 1992
Broad-host-range plasmids carrying alpha-amylase or beta-lactamase reporter genes lacking a signal sequence were used to select export elements from Lactococcus lactis chromosomal DNA that could function as signal sequences. Fragments containing such elements were identified by their ability to direct the export of the reporter proteins in Escherichia ...
Perez-Martinez, Gaspar, Kok, Jan, Venema, Gerhardus, Dijl, Jan Maarten van, Smith, Hilda, Bron, Sierd   +5 more
openaire   +2 more sources

Fluorescence assessment of Lactococcus lactis viability

International Journal of Food Microbiology, 2000
The reproduction and activity of lactic acid bacteria (LAB) are essential in their applications in the dairy industry and other fermentations. Traditionally used methods like plate counting and acidification tests require long incubation times and provide limited information.
Bunthof, C.J., van den Braak, S., Breeuwer, P., Rombouts, F.M., Abee, T.   +4 more
openaire   +2 more sources