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Freeze-Fracture Study of the Plasma Membrane of Leishmania mexicana amazonensis

The Journal of Parasitology, 1980
The fine structure of the plasma membrane of promastigotes of Leishmania mexicana amazonensis was studied by freeze-fracture techniques. Measurement of the number of intramembranous particles per square micrometer of membrane area revealed a particle density of 2,040 +/- 213/micron2 on the P-face and 890 +/- 186/micron2 on the E-face.
M, Benchimol, W, de Souza
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Sequence analysis and transcriptional activation of heat shock protein 83 of Leishmania mexicana amazonensis

Molecular and Biochemical Parasitology, 1990
Changes in environmental temperature regulate the differential expression of genes during Leishmania stage differentiation. Therefore, molecular analysis of the heat shock proteins (HSPs) in these parasites is of interest as a model for thermoregulation of gene expression.
M, Shapira, G, Pedraza
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Cytochemical localization of acid phosphatase in Leishmania mexicana amazonensis.

Journal of submicroscopic cytology, 1986
Acid phosphatase was cytochemically detected at the ultrastructural level in infective and non-infective promastigotes and in amastigotes of the parasitic protozoan Leishmania mexicana amazonensis. Cerium chloride was used as the capture agent of the phosphate liberated during the hydrolysis of the substrate (Na-beta-glycerophosphate). Reaction product,
P F, Pimenta, W, De Souza
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Granulocyte-Macrophage and Macrophage Colony-Stimulating Factors Activate Intramacrophage Killing of Leishmania mexicana amazonensis

Journal of Infectious Diseases, 1990
Leishmania organisms are important pathogens, causing diseases worldwide. Standard therapies are often toxic and are not always effective. The effect of recombinant human granulocyte-macrophage and macrophage colony-stimulating factors (GM-CSF and M-CSF) on intramacrophage survival of Leishmania mexicana amazonensis (Lma) were compared with those of ...
J L, Ho   +3 more
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A plasma membrane P-type H+-ATPase regulates intracellular pH in Leishmania mexicana amazonensis

Molecular and Biochemical Parasitology, 2002
A recent report (Mukherjee et al., J. Biol. Chem. 276 (2001) 5563) has proposed that the plasma membrane Mg(+)-ATPase of promastigotes of Leishmania donovani, that is involved in its intracellular pH regulation, is an electroneutral H(+)/K(+) antiporter rather than an electrogenic H(+) pump. Since this proposition has important implications for the use
Norma, Marchesini, Roberto, Docampo
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Isolation of the intracellular stages ofLeishmania mexicana amazonensisusing cellulose column

Annals of Tropical Medicine & Parasitology, 1978
(1978). Isolation of the intracellular stages of Leishmania mexicana amazonensis using cellulose column. Annals of Tropical Medicine & Parasitology: Vol. 72, No. 6, pp. 579-580.
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Isolation and Purification of Amastigotes of Leishmania mexicana amazonensis by a Gradient of Metrizamide

The Journal of Parasitology, 1983
Some attempts have been made to isolate intracellular forms of cutaneous leishmaniasis (Childs et al., 1976, J. Parasitol. 62: 676-679; Shaw and Lainson, 1977, J. Parasitol. 63: 384385; Brasil, 1978, Ann. Trop. Med. Parasitol. 72: 379-580; Infante et al., 1980, Parasitology 80:105-112). However, each of these techniques has limitations.
E M, Saraiva   +3 more
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Effects of temperature elevation on mRNA and protein synthesis in Leishmania mexicana amazonensis

Molecular and Biochemical Parasitology, 1988
The transition from the promastigote stage to the amastigote stage in Leishmania appears to involve a sequence of steps which enable the parasite to adapt to its new environment. In this study, transformation from the promastigote to an amastigote-like stage was induced by temperature elevation and the effects on protein synthesis and the mRNA ...
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Status of respiration and ATP content in arsenite resistantLeishmania mexicana amazonensis

Microbial Pathogenesis, 1999
Cellular ATP and rate of respiration are important for the cell survival. We have analyzed both the parameters in wild type and arsenite resistant Leishmania mexicana amazonensis. There was no significant change observed in the rate of respiration and cellular ATP content between drug resistant cells (resistance to 30 microM of sodium arsenite) and ...
A K, Singh, S T, Lee
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Specific immunization of mice against Leishmania mexicana amazonensis using solubilized promastigotes.

Clinical and experimental immunology, 1987
Successful immunization of highly susceptible BALB/c mice against progressive infection by Leishmania mexicana amazonensis, using whole solubilized promastigotes was achieved. The best immunization schedule consisted of three weekly injections of 5 X 10(7) parasite equivalents.
M, Barral-Netto   +3 more
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