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[Transduction of hematopoietic stem cells by lentiviral vectors].
Uirusu, 2003 openaire +1 more source
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Lentiviral Transduction of Neuronal Cells
2021Lentiviruses are a very reliable class of viral vectors wildly used in gene therapy. In this chapter, we described a general method for the construction of lentiviral delivery system by using a derived HIV-1 based lentivirus expression vector pKLV-Puro containing a monomeric blue fluorescent protein mammalian codon-optimized (TagBFP).
Anna, Bellizzi +2 more
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Lentiviral Gene Transduction of Kidney
Human Gene Therapy, 2002Gene transfer into kidney holds great potential as a novel therapeutic approach. We have studied the transduction of kidney in vivo after delivery of lentiviral vectors by various routes of administration. A lentiviral vector expressing the bacterial lacZ gene from the cytomegalovirus early promoter was used.
G Luca, Gusella +5 more
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Lentiviral Transduction of Cultured Microglia
2013Microglial cells are the resident immune-related glial cells of the central nervous system (CNS) that are crucial for maintaining homeostasis and sensing pathological alterations in the nervous system. To improve our understanding of the biological function of microglia, gene-transfer techniques have been improved and become widely used over the past ...
Takahiro, Masuda +3 more
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Lentiviral Transduction of Mammary Epithelial Cells
2022Lentiviral vectors are the workhorses of modern cell biology. They can infect a wide variety of cells including non-dividing cells and stem cells. They integrate into the genome of infected cells leading to stable expression. It is easy to transduce 100% of the cells in a culture and possible to infect cells simultaneously with multiple vectors ...
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Lentiviral Transduction of Immune Cells
2008Gene transfer into mammalian cells has been of crucial importance for studies determining the role of specific genes in the differentiation and cell fate of various hematopoietic lineages. Until recently, the majority of these studies were performed in transformed cell lines due to difficulties in achieving levels of transfection of greater than 1-3 ...
Louise, Swainson +4 more
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Lentiviral transduction of murine oligodendrocytes in vivo
Journal of Neuroscience Research, 2005Lentiviral vectors are used widely to direct efficient gene transfer in vivo. We examined cell-specific expression in adult murine white matter after stereotaxic microinjection of four lentiviral constructs. We synthesized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviruses with combinations of two promoters, cytomegalovirus (CMV ...
Sally R, McIver +6 more
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Lentiviral Transduction of Human Dendritic Cells
2004Dendritic cells (DCs) are potent antigen-presenting cells (APCs) that play a pivotal role in stimulating antigen-specific T cells in vivo. The cardinal properties of DCs are: the ability to take up, process, and present antigens; (2) the ability to migrate through different tissues into lymphoid organs; and (3) the ability to interact with and ...
Roland, Schroers, Si-Yi, Chen
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Retroviral/Lentiviral Transduction and Transformation Assay
2009Non-random chromosomal translocations can be found in about half of acute leukaemia patients and mostly lead to either over-expression of proto-oncogenes or creation of novel fusion genes. To assess the oncogenic potential and characterize the underlying mechanisms mediated by these candidate oncoproteins, a retroviral transduction/transformation assay
Bernd B, Zeisig, Chi Wai Eric, So
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Lentiviral Transduction of Mammary Epithelial Cells
2015Lentiviral vectors are the workhorses of modern cell biology. They can infect a wide variety of cells including nondividing cells and stem cells. They integrate into the genome of infected cells leading to stable expression. It is easy to transduce 100 % of the cells in a culture and possible to infect cells simultaneously with multiple vectors ...
Richard, Iggo, Elodie, Richard
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