Results 291 to 300 of about 116,783 (314)

Transduction of Human Islets with Pseudotyped Lentiviral Vectors

Human Gene Therapy, 2004
Type I diabetes is caused by an autoimmune-mediated elimination of insulin-secreting pancreatic islets. Genetic modification of islets offers a powerful molecular tool for improving our understanding of islet biology. Moreover, efficient genetic engineering of islets could allow for evaluation of new strategies aimed at preventing islet destruction ...
Gary P, Kobinger, Shaoping, Deng, Jean-Pierre, Louboutin, Marko, Vatamaniuk, Franz, Matschinsky, James F, Markmann, Steven E, Raper, James M, Wilson   +7 more
openaire   +2 more sources

Lentiviral vector-mediated transduction of goat undifferentiated spermatogonia

Animal Reproduction Science, 2015
Recent studies show that spermatogonial stem cells (SSCs) are able to colonize and form mature spermatozoa following transplantation into germ cell depleted testes of recipient males. Therefore, efficient ways for enrichment and gene transfer into SSCs provides a powerful tool for production of transgenic animals.
Hassan, Abbasi, Sayyed Morteza, Hosseini, Mahdi, Hajian, Zahra, Nasiri, Mehrnoosh, Bahadorani, Mojtaba, Tahmoorespur, Mohammad Reza, Nasiri, Mohammad Hossein, Nasr-Esfahani   +7 more
openaire   +2 more sources

Transduction of human islets with the lentiviral vector

Transplantation Proceedings, 2004
Establishment of an efficient gene delivery system for human pancreatic beta cells is important for the development of diabetes-targeted cell therapies. The human immunodeficiency virus type 1 (HIV-1)-derived lentiviral vector is well documented to be an effective gene transfer tool for various types of cells.
N, Kobayashi, T, Arata, T, Okitsu, H, Ikeda, K, Kobayashi, Y, Kosaka, M, Narushima, N, Tanaka, J R T, Lakey   +8 more
openaire   +2 more sources

Generation of CAR-T Cells by Lentiviral Transduction

2021
CAR-T cell therapy is one of the most successful cell-based therapies. T cells are the most common cells to be genetically modified for cancer therapy, not only because T cells have cytotoxicity but also because they are easily cultured ex vivo and genetically modified with viral vectors.
openaire   +2 more sources

Stable Expression by Lentiviral Transduction of Cells

2018
Lentiviral gene transfer represents a versatile and powerful method for genetic transduction of many cell lines and primary cells including "hard-to-transfect" cells. As a consequence of the integration of the recombinant lentiviral vector into the cellular genome, the transgene is stably maintained, and long-term producing cells are established. Here,
Natascha, Gödecke, Hansjörg, Hauser, Dagmar, Wirth   +2 more
openaire   +3 more sources

Magnetically guided lentiviral‐mediated transduction of airway epithelial cells

The Journal of Gene Medicine, 2010
AbstractBackgroundLentiviral (LV) vectors are able to only slowly and inefficiently transduce nondividing cells such as those of the airway epithelium. To address this issue, we have exploited the magnetofection technique in in vitro models of airway epithelium.MethodsMagnetofectins were formed by noncovalent interaction between LV particles and ...
C. ORLANDO C, CASTELLANI, STEFANO, O. MYKHAYLYK, E. COPRENI, O. ZELPHATI, C. PLANK, CONESE, MASSIMO   +6 more
openaire   +2 more sources

Improving Lentiviral Transduction Efficiency with Microfluidic Systems

Blood, 2015
Abstract Background: Recent clinical trials have demonstrated the efficacy and safety of gene therapy utilizing HIV-derived lentiviral vectors (LVs) for blood disorders. However, the LV requirements and clinical ex vivo cell transduction protocols used in these studies exposes the limitations of the technology and beckons the need for ...
Reginald Tran, David R Myers, Jordan E Shields, Byungwook Ahn, Yongzhi Qiu, Caroline Hansen, Yumiko Sakurai, Robert Moot, H. Trent Spencer, Christopher B Doering, Wilbur A Lam   +10 more
openaire   +1 more source

Lentiviral Transduction of Primary Human Glioblastoma Cultures

2018
This chapter provides detailed step-by-step instructions for the production of lentiviral particles and the transduction of primary human glioblastoma cultures. Lentiviruses stably transduce both dividing and non-dividing cells, such as quiescent cancer stem cell populations.
Joshua D, Frenster, Julio, Inocencio, Dimitris G, Placantonakis   +2 more
openaire   +2 more sources

Lentiviral Transduction for Optimal LSC/HSC Manipulation

2020
Historically, efficient transduction of hematopoietic stem cells (HSC) to study the role of specific genes on HSC function, as well as to broaden the potential of gene therapy for hematopoietic related diseases has relied on our ability to design vectors capable of delivering the gene of interest without affecting HSC function.
openaire   +2 more sources

Poloxamer synperonic F108 improves cellular transduction with lentiviral vectors

The Journal of Gene Medicine, 2012
AbstractBackgroundAlthough lentiviral transduction methods are widely used, their broader application is dependent upon the optimization of lentiviral transduction efficiency for a broad range of cell types. In the present study, we focus on the evaluation of two chemical classes with respect to their ability to increase lentiviral transduction without
Ines, Höfig, Michael J, Atkinson, Sabine, Mall, Angela M, Krackhardt, Christian, Thirion, Nataša, Anastasov   +5 more
openaire   +2 more sources