Results 71 to 80 of about 172,662 (343)

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Identifying the Conditions Under Which Antibodies Protect Against Infection by Equine Infectious Anemia Virus

Vaccines, 2014
The ability to predict the conditions under which antibodies protect against viral infection would transform our approach to vaccine development. A more complete understanding is needed of antibody protection against lentivirus infection, as well as the ...
Elissa J. Schwartz, Robert J. Smith?
doaj   +1 more source

Lentivirus Nef Specifically Activates Pak2 [PDF]

Journal of Virology, 2000
ABSTRACT Nef proteins from human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) have been found to associate with an active cellular serine/threonine kinase designated Nef-associated kinase (Nak).
V K, Arora, R P, Molina, J L, Foster, J L, Blakemore, J, Chernoff, B L, Fredericksen, J V, Garcia   +6 more
openaire   +2 more sources

RNA‐Micelles as Self‐Assembling Structures for Efficient Co‐Delivery of Synergistic siRNA and Nucleoside Analogues to Treat CRC Lung Metastasis

Advanced Functional Materials, EarlyView.
Two kinds of self‐assembled RNA micelles were used to co‐deliver synergistic siRNA and nucleoside analogues for the treatment of colorectal cancer lung metastases. Near‐complete elimination of lung cancer metastases was confirmed in an orthotopic lung metastasis model constructed using human colorectal cancer lung metastases patient surgical samples to
Kai Jin, Piotr Rychahou, Daniel W. Binzel, B. Mark Evers, Peixuan Guo   +4 more
wiley   +1 more source

Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography

Veterinární Medicína, 2001
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal binding domain in pRSET-B vector.
D. Molinková
doaj   +1 more source

Nano‐ and Micro‐Sized Solid Materials Used as Antiviral Agents

Advanced Functional Materials, EarlyView.
Due to the rise of viral infections in humans and possible viral outbreaks, the use of nano‐ or micro‐sized materials as antiviral agents is rapidly increasing. This review explores their antiviral properties against RNA and DNA viruses, either as a prevention or a treatment tool, by delving into their mechanisms of action and how to properly assess ...
Orfeas‐Evangelos Plastiras, Imelda Raczkiewicz, Peggy Bouquet, Jean‐Philippe Dacquin, Jeremy Dhainaut, Anne Goffard, Christophe Volkringer   +6 more
wiley   +1 more source

Sources of Error in Mammalian Genetic Screens

G3: Genes, Genomes, Genetics, 2016
Genetic screens are invaluable tools for dissection of biological phenomena. Optimization of such screens to enhance discovery of candidate genes and minimize false positives is thus a critical aim.
Laura Magill Sack, Teresa Davoli, Qikai Xu, Mamie Z. Li, Stephen J. Elledge   +4 more
doaj   +1 more source

Femtosecond‐Laser‐Fabricated 3D CYTOP Microfluidic Chips Enabling Super‐Resolution Live Imaging of Cell Behavior in Confined Microspaces

Advanced Materials Technologies, EarlyView.
Using two‐photon polymerization of SU‐8 together with mold processing allowed the creation of defect‐free, flexible 3D microfluidic structures with sub‐micrometer precision in CYTOP. The fabricated CYTOP microfluidic chips enabled super‐resolution imaging of cancer cells in the microchannels, clearly visualizing previously undetectable fine structural ...
Koji Sugioka, Mirai Hanzawa, Kotaro Obata, Sota Matsumoto, Masatoshi Takagi, Asako Sakaue‐Sawano, Asako Tosaki, Satoshi Shimozono, Felix Sima, Hiroyuki Kawano, Takuro Tojima, Daisuke Miyashiro, Kazunari Ozasa, Yasutaka Hanada, Godai Miyaji, Akihiko Nakano, Atsushi Miyawaki   +16 more
wiley   +1 more source

Impact of Veterinary Vaccines on Human Health and Probable Challenges

Acta Veterinaria Eurasia, 2021
Md. Mehadi Hasan SOHAG, Yahia CHEBLOUNE   +1 more
doaj   +1 more source

Phenotypic lentivirus screens to identify functional single domain antibodies

Nature Microbiology, 2016
Manipulation of proteins is key in assessing their in vivo function. Although genetic ablation is straightforward, reversible and specific perturbation of protein function remains a challenge.
F. Schmidt, L. Hanke, B. Morin, R. Brewer, Vesna Brusic, S. Whelan, H. Ploegh   +6 more
semanticscholar   +1 more source