Results 251 to 260 of about 67,942 (278)
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Validation of the anthrax lethal toxin neutralization assay
Biologicals, 2004A validation of the performance characteristics of a toxin neutralization assay is presented. This in vitro assay measures the functional ability of antisera, containing antibodies to anthrax lethal toxin, to specifically protect J774A.1 cells against Bacillus anthracis lethal toxin cytotoxicity.
Donna, Hering +5 more
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Disulfide Bonds of Purothionin, a Lethal Toxin for Yeasts
The Journal of Biochemistry, 1978Purothionin isolated from commercial wheat flour contained several components and two of them (A-I and A-II) were isolated in pure form by CM-52 column chromatography. Each component contained 45 amino acid residues with a 4 disulfide bonds. Purothionin A-II was digested with trypsin and thermolysin to isolate cystine peptides. These were separated and
T, Hase, H, Matsubara, H, Yoshizumi
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Purification of a lethal toxin produced by Shigella dysenteriae
Toxicon, 1982A lethal toxin was purified from the culture supernatant of Shigella dysenteriae 1. The purification procedure utilized ammonium sulfate fractionation, column chromatography on DEAE-cellulose, CM-cellulose, hydroxylapatite and gel filtration on Sephadex G-200. About a 4760-fold purification was achieved, with a yield of 2.7%.
K, Okamoto, Y, Takeda, T, Miwatani
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Anthrax lethal toxin: a weapon of multisystem destruction
Cellular and Molecular Life Sciences, 2004Lethal toxin (LT) is a major virulence factor secreted by anthrax bacteria. It is composed of two proteins, PA (protective antigen) and LF (lethal factor). PA transports the LF inside the cell, where LF, a zinc-dependent metalloprotease cleaves the mitogen activated protein kinase kinase (MAPKK) enzymes of the mitogen activated protein kinase (MAPK ...
A, Agrawal, B, Pulendran
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The Pathogenesis of the Lethal Effect of Anthrax Toxin in the Rat
Journal of Infectious Diseases, 1966ditions previously described (Beall et al, 1962). After 24 hours growth the bulk of the bacteria was removed by centrifugation, and the crude or unfractionated toxin used in these experiments was prepared in 1 of 2 ways: (a) the culture was sterilized by filtration through Millipore filters in stainless steel funnels, frozen, and stored at ?20 C; or (b)
F A, Beall, F G, Dalldorf
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Molecular Microbiology, 1994
SummaryComparison of the anthrax toxin lethal factor (LF) amino acid sequence with sequences in the Swiss protein database revealed short regions of similarity with the consensus zinc‐binding site, HEXXH, that is characteristic of metalloproteases. Several protease inhibitors, including bestatin and captopril, prevented intoxication of macrophages by ...
K R, Klimpel, N, Arora, S H, Leppla
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SummaryComparison of the anthrax toxin lethal factor (LF) amino acid sequence with sequences in the Swiss protein database revealed short regions of similarity with the consensus zinc‐binding site, HEXXH, that is characteristic of metalloproteases. Several protease inhibitors, including bestatin and captopril, prevented intoxication of macrophages by ...
K R, Klimpel, N, Arora, S H, Leppla
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Crystallographic studies of the anthrax lethal toxin
Journal of Applied Microbiology, 1999Anthrax lethal toxin comprises two proteins: protective antigen (PA; MW 83 kDa) and lethal factor (LF; MW 87 kDa). We have recently determined the crystal structure of the 735-residue PA in its monomeric and heptameric forms (Petosa et al. 1997). It bears no resemblance to other bacterial toxins of known three-dimensional structure, and defines a new ...
, Liddington +4 more
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Protein and Peptide Letters, 2012
Lethal toxin of Clostridium sordellii (MLD 150 ng/kg) is one of the most potent Clostridial toxins and is responsible for most of the diseases including sudden death syndrome in cattle, sheep and toxic shock syndrome, necrotizing faciitis, neonatal omphalitis and gangrene in humans. Lethal toxin (TcsL) is a single chain protein of about 270 kDa. In the
Preetika, Arya +3 more
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Lethal toxin of Clostridium sordellii (MLD 150 ng/kg) is one of the most potent Clostridial toxins and is responsible for most of the diseases including sudden death syndrome in cattle, sheep and toxic shock syndrome, necrotizing faciitis, neonatal omphalitis and gangrene in humans. Lethal toxin (TcsL) is a single chain protein of about 270 kDa. In the
Preetika, Arya +3 more
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Anthrax lethal toxin (Bacillus anthracis)
1997Abstract Anthrax lethal toxin (LeTx) induces shock and sudden death in test animals, mimicking the symptoms of systemic anthrax infections (eppla 1991; Hanna et al. 1993). Bacterial strains lacking either of the two LeTx components are highly attenuated, and immunization against the toxin protects animals from 8.
P C Hanna, R J Collier
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Novel Repression of the Glucocorticoid Receptor by Anthrax Lethal Toxin
Annals of the New York Academy of Sciences, 2004Abstract: Death from anthrax has been reported to occur from systemic shock. The lethal toxin (LeTx) is the major effector of anthrax mortality. Although the mechanism of entry of this toxin into cells is well understood, its actions once inside the cell are not as well understood. LeTx is known to cleave and inactivate MAPKKs.
Jeanette I, Webster +2 more
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