Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy
eLife, 2020 As the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models.
Claudia Prahst +16 more
doaj +1 more source
An integrated single- and two-photon non-diffracting light-sheet microscope
, 2017 We describe the apparatus of a fluorescence optical microscope with both single-photon and two-photon non-diffracting light sheets excitation for large volume imaging.
Chiu, Hoi Chun +5 more
core +1 more source
Multifunctional Microstructured Surfaces by Microcontact Printing of Reactive Microgels
Advanced Functional Materials, EarlyView.Reactive poly(N‐vinylcaprolactam‐co‐glycidyl methacrylate) microgels are used as functional inks to create surface‐grafted arrays on glass via microcontact printing. The patterns (10–50 µm widths and spacings) enable stable binding and post‐functionalization with dyes and peptides.
Inga Litzen +4 more
wiley +1 more source
Recent progress of second near-infrared (NIR-II) fluorescence microscopy in bioimaging
Frontiers in Physiology, 2023 Visualizing biological tissues in vivo at a cellular or subcellular resolution to explore molecular signaling and cell behaviors is a crucial direction for research into biological processes.
Tian Wang +3 more
doaj +1 more source
Global Connectivity and Function of Descending Spinal Input Revealed by 3D Microscopy and Retrograde Transduction [PDF]
, 2018 The brain communicates with the spinal cord through numerous axon tracts that arise from discrete nuclei, transmit distinct functions, and often collateralize to facilitate the coordination of descending commands.
Blackmore, Murray G. +4 more
core +2 more sources
Advanced Functional Materials, EarlyView.Two‐photon lithography (TPL) enables 3D magnetic nanostructures with unmatched freedom in geometry and material choice. Advances in voxel control, deposition, and functionalization open pathways to artificial spin ices, racetracks, microrobots, and a number of additional technological applications.
Joseph Askey +5 more
wiley +1 more source
Beam shaping in light-sheet microscopy: an experimental analysis
JPhys PhotonicsThanks to its unique optical sectioning capability, light-sheet fluorescence microscopy has proven to be a powerful technique for volumetric imaging of entire model organisms with high spatial and temporal resolution.
Manuel Hüpfel, Gerd Ulrich Nienhaus
doaj +1 more source
Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy
Frontiers in Cellular Neuroscience, 2014 The development of three dimensional cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type interactions in a complex three ...
Emilio J Gualda +8 more
doaj +1 more source
Shadow imaging for panoptical visualization of brain tissue in vivo
Nature Communications, 2023 Progress in neuroscience research hinges on technical advances in visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require tissue fixation (electron microscopy), do not have cellular ...
Yulia Dembitskaya +17 more
doaj +1 more source
Isotropic reconstruction of 3D fluorescence microscopy images using convolutional neural networks
, 2017 Fluorescence microscopy images usually show severe anisotropy in axial versus lateral resolution. This hampers downstream processing, i.e. the automatic extraction of quantitative biological data.
A Jog +11 more
core +1 more source