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Innovations in ex vivo Light Sheet Fluorescence Microscopy [PDF]
Progress in Biophysics and Molecular Biology, 2022 Light Sheet Fluorescence Microscopy (LSFM) has revolutionized how optical imaging of biological specimens can be performed as this technique allows to produce 3D fluorescence images of entire samples with a high spatiotemporal resolution. In this manuscript, we aim to provide readers with an overview of the field of LSFM on ex vivo samples.
Pablo Delgado-Rodriguez +3 more
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Light Sheet Fluorescence Microscopy (LSFM) [PDF]
Current Protocols in Cytometry, 2015 Abstract The development of confocal microscopy techniques introduced the ability to optically section fluorescent samples in the axial dimension, perpendicular to the image plane. These approaches, via the placement of a pinhole in the conjugate image plane, provided superior resolution in the axial (z) dimension resulting in nearly ...
Michael W, Adams +4 more
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Three-photon light-sheet fluorescence microscopy [PDF]
Optics Letters, 2018 We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low photo-damage technique for fast volumetric imaging of biological samples.
Adrià Escobet-Montalbán +5 more
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Light-sheets and smart microscopy, an exciting future is dawning
Communications Biology, 2023 Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods.
Stephan Daetwyler, Reto Paul Fiolka
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3D Volume Rendering of Invertebrates Using Light-Sheet Fluorescence Microscopy
Photonics, 2022 Light-Sheet Fluorescence Microscopy has recently emerged as the technique of choice for obtaining high quality three-dimensional (3D) images of whole organisms, with low photo-damage and fast acquisition rates.
Emilio J. Gualda
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Light sheet fluorescence microscopy for neuroscience [PDF]
Journal of Neuroscience Methods, 2019 The functions of the central nervous system (CNS) rely on the interaction between large populations of neurons across different areas. Therefore, to comprehend CNS functions there is a need for imaging techniques providing access to the neuronal activity of large networks of neurons with very high spatiotemporal resolution.Light sheet fluorescence ...
Corsetti, Stella +2 more
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Light Sheet Fluorescence Microscopy [PDF]
Journal of Histochemistry & Cytochemistry, 2011 Light sheet fluorescence microscopy (LSFM) functions as a non-destructive microtome and microscope that uses a plane of light to optically section and view tissues with subcellular resolution. This method is well suited for imaging deep within transparent tissues or within whole organisms, and because tissues are exposed to only a thin plane of light,
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Visualizing the Invisible: Advanced Optical Microscopy as a Tool to Measure Biomechanical Forces
Frontiers in Cell and Developmental Biology, 2021 The importance of mechanical force in biology is evident across diverse length scales, ranging from tissue morphogenesis during embryo development to mechanotransduction across single adhesion proteins at the cell surface.
Chad M. Hobson +3 more
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Frontiers in Plant Science, 2020 Annexin 1 (ANN1) is the most abundant member of the evolutionary conserved multigene protein superfamily of annexins in plants. Generally, annexins participate in diverse cellular processes, such as cell growth, differentiation, vesicle trafficking, and ...
Michaela Tichá +7 more
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Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow-driven samples in a light-sheet fluorescence microscope [PDF]
EPJ Web of Conferences, 2022 Light-sheet fluorescence microscopy (LSFM) with single light-sheet illumination enables rapid 3Dimaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets ...
Hofmann Meike +5 more
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