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Light Sheet Fluorescence Microscopy
Proceeding of the Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020, 2020 In light-sheet fluorescence microscopy (LSFM) the optical axes of the image-forming microscope objective and the objective providing illumination of the specimen are at right angles to one another (Olarte et al., 2018). A sheet of light is incident on the specimen, and fluorescence is produced and hence detected only in that plane.
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Nonlinear light-sheet fluorescence microscopy by photobleaching imprinting [PDF]
Journal of The Royal Society Interface, 2014 We present a nonlinear light-sheet fluorescence microscopy (LSFM) scheme based on photobleaching imprinting. By measuring photobleaching-induced fluorescence decay, our method simultaneously achieves a large imaging field of view and a thin optical section.
Gao, Liang +3 more
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Removing striping artifacts in light-sheet fluorescence microscopy: a review [PDF]
Progress in Biophysics and Molecular Biology, 2022 In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to point-scanning methods.
Ricci P. +7 more
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Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser
Methods and Protocols, 2019 Light sheet fluorescence microscopy techniques have revolutionized biological microscopy enabling low-phototoxic long-term 3D imaging of living samples. Although there exist many light sheet microscopy (LSM) implementations relying on fluorescence, just ...
Diego Di Battista +4 more
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Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy [PDF]
EPJ Web of Conferences, 2023 Root hairs are a delicate single-cell system whose growth is regulated by a fine mechanism characterised by the presence of a tip-high Ca2+ gradient that shows regular oscillations in growing root hairs.
Tortora Giorgia +5 more
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Charting Monosynaptic Connectivity Maps by Two-Color Light-Sheet Fluorescence Microscopy
Cell Reports, 2012 Cellular resolution three-dimensional (3D) visualization of defined, fluorescently labeled long-range neuronal networks in the uncut adult mouse brain has been elusive.
Christian J. Niedworok +5 more
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Frontiers in Neuroanatomy, 2021 The combination of tissue clearing techniques with advanced optical microscopy facilitates the achievement of three-dimensional (3D) reconstruction of macroscopic specimens at high resolution.
Marina Scardigli +19 more
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Multi-photon attenuation-compensated light-sheet fluorescence microscopy [PDF]
Scientific Reports, 2019 Attenuation of optical fields owing to scattering and absorption limits the penetration depth for imaging. Whilst aberration correction may be used, this is difficult to implement over a large field-of-view in heterogeneous tissue. Attenuation-compensation allows tailoring of the maximum lobe of a propagation-invariant light field and promises an ...
Veettikazhy, Madhu +7 more
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Photonics, 2021 Stimuli to excitable cells and various cellular processes can cause cell surface deformations; for example, when excitable cell membrane potentials are altered during action potentials.
Mariana Potcoava +3 more
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Cells, 2021 Cortical dysplasias are alterations in the organization of the layers of the brain cortex due to problems in neuronal migration during development. The neuronal component has been widely studied in experimental models of cortical dysplasias. In contrast,
Cynthia Alejandra Rodríguez-Arzate +5 more
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