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Light sheet fluorescence microscopy
Nature Reviews Methods Primers, 2021Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical sectioning capability and, hence, provide axial resolution, restrict photobleaching and phototoxicity to a fraction of the sample and use cameras to record tens to thousands of ...
Adam Glaser, Emmanuel G Reynaud
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Single-objective light sheet microscopy
Nature Methods, 2021Single-objective light sheet fluorescence microscopes are driving innovation in volumetric imaging.
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Quantifiable Intravital Light Sheet Microscopy
2022Live imaging of zebrafish embryos that maintains normal development can be difficult to achieve due to a combination of sample mounting, immobilization, and phototoxicity issues that, once overcome, often still results in image quality sufficiently poor that computer-aided analysis or even manual analysis is not possible. Here, we describe our mounting
Holly C, Gibbs +7 more
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2014
This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an alternative to confocal microscopy due to several superior features such as high-speed full-frame acquisition, minimal phototoxicity, and multiview sample ...
Weber, M., Mickoleit, M., Huisken, J.
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This chapter introduces the concept of light sheet microscopy along with practical advice on how to design and build such an instrument. Selective plane illumination microscopy is presented as an alternative to confocal microscopy due to several superior features such as high-speed full-frame acquisition, minimal phototoxicity, and multiview sample ...
Weber, M., Mickoleit, M., Huisken, J.
openaire +3 more sources
Fast Fluorescence Microscopy with Light Sheets
The Biological Bulletin, 2016In light sheet microscopy, optical sectioning by selective fluorescence excitation with a sheet of light is combined with fast full-frame acquisition. This illumination scheme provides minimal photobleaching and phototoxicity. Complemented with remote focusing and multi-view acquisition, light sheet microscopy is the method of choice for acquisition of
Daetwyler, S., Huisken, J.
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Resolution upgrades for light-sheet microscopy
Nature Methods, 2019Single objective light-sheet fluorescence microscopes combine the convenience of conventional sample mounting with sensitive subcellular and super-resolution imaging of cells and tissues.
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An eye on light-sheet microscopy
2016This chapter introduces the principles and advantages of selective plane illumination microscopy (SPIM) and compares it to commonly used epifluorescence or confocal setups. Due to the low phototoxicity, speed of imaging, high penetration depth, and spatiotemporal resolution, SPIM is predestined for in vivo imaging but can as well be used for in toto ...
D. Kromm, T. Thumberger, J. Wittbrodt
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Light Sheet Microscopy in Cell Biology
2012Light sheet-based fluorescence microscopy (LSFM) is emerging as a powerful imaging technique for the life sciences. LSFM provides an exceptionally high imaging speed, high signal-to-noise ratio, low level of photo-bleaching, and good optical penetration depth.
Raju, Tomer +2 more
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Planning for post‐pandemic cancer care delivery: Recovery or opportunity for redesign?
Ca-A Cancer Journal for Clinicians, 2021Pelin Cinar +2 more
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