Results 61 to 70 of about 218,213 (290)
Frontiers in Cellular Neuroscience, 2021 Until recently, the visualization of cerebral microvessels was hampered by the fact that only short segments of vessels could be evaluated in brain sections by histochemistry.
Ayan Mohamud Yusuf +12 more
doaj +1 more source
OpenSPIM - an open access platform for light sheet microscopy
, 2013 Light sheet microscopy promises to revolutionize developmental biology by enabling live in toto imaging of entire embryos with minimal phototoxicity. We present detailed instructions for building a compact and customizable Selective Plane Illumination ...
A Edelstein +13 more
core +1 more source
Adaptable, illumination patterning light sheet microscopy [PDF]
Scientific Reports, 2018 AbstractMinimally-invasive optical imaging requires that light is delivered efficiently to limit the detrimental impact of photodamage on delicate biological systems. Light sheet microscopy represents the exemplar in tissue specific optical imaging of small and mesoscopic samples alike.
Rory M. Power, Jan Huisken
openaire +4 more sources
Potential therapeutic targeting of BKCa channels in glioblastoma treatment
Molecular Oncology, EarlyView.This review summarizes current insights into the role of BKCa and mitoBKCa channels in glioblastoma biology, their potential classification as oncochannels, and the emerging pharmacological strategies targeting these channels, emphasizing the translational challenges in developing BKCa‐directed therapies for glioblastoma treatment.
Kamila Maliszewska‐Olejniczak +4 more
wiley +1 more source
Multimode fibre:Light-sheet microscopy at the tip of a needle [PDF]
, 2015 We also thank the UK Engineering and Physics Sciences Research Council for funding under grant EP/J01771X/1. Finally, we would like to thank EXCELLENT TEAMS (CZ.1.07/2.3.00/30.0005) from European Social Fund and CEITEC - Central European Institute of ...
AA Friesem +29 more
core +2 more sources
Light sheet microscopy for histopathology applications [PDF]
Biomedical Engineering Letters, 2019 Light sheet microscopy (LSM) is an evolving optical imaging technique with a plane illumination for optical sectioning and volumetric imaging spanning cell biology, embryology, and in vivo live imaging. Here, we focus on emerging biomedical applications of LSM for tissue samples.
Praveen Kumar Poola +4 more
openaire +3 more sources
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Imaging the developing human external and internal urogenital organs with light sheet fluorescence microscopy. [PDF]
, 2020 Technological advances in three-dimensional (3D) reconstruction techniques have previously enabled paradigm shifts in our understanding of human embryonic and fetal development. Light sheet fluorescence microscopy (LSFM) is a recently-developed technique
Baskin, Laurence +9 more
core
Fast imaging of live organisms with sculpted light sheets. [PDF]
, 2015 Light-sheet microscopy is an increasingly popular technique in the life sciences due to its fast 3D imaging capability of fluorescent samples with low photo toxicity compared to confocal methods.
Chmielewski, Aleksander K +6 more
core +3 more sources
Deconvolution of light sheet microscopy recordings [PDF]
Scientific Reports, 2019 AbstractWe developed a deconvolution software for light sheet microscopy that uses a theoretical point spread function, which we derived from a model of image formation in a light sheet microscope. We show that this approach provides excellent blur reduction and enhancement of fine image details for image stacks recorded with low magnification ...
Klaus Becker +7 more
openaire +2 more sources