Membrane Fusion Assay Based on Pore-Spanning Lipid Bilayers [PDF]
Biophysical Journal, 2010 Pore-spanning planar membranes on highly ordered porous silicon substrates were shown to be well suited to monitor the calcium ion mediated fusion of large unilamellar vesicles by means of confocal laser scanning fluorescence microscopy and scanning ion conductance microscopy in real-time.
Höfer, Ines, Steinem, Claudia
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Atomic-resolution simulations predict a transition state for vesicle fusion defined by contact of a few lipid tails. [PDF]
PLoS Computational Biology, 2010 Membrane fusion is essential to both cellular vesicle trafficking and infection by enveloped viruses. While the fusion protein assemblies that catalyze fusion are readily identifiable, the specific activities of the proteins involved and nature of the ...
Peter M Kasson +2 more
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Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion. [PDF]
, 2012 The molecular underpinnings of synaptic vesicle fusion for fast neurotransmitter release are still unclear. Here, we used a single vesicle-vesicle system with reconstituted SNARE and synaptotagmin-1 proteoliposomes to decipher the temporal sequence of ...
Brunger, Axel T +13 more
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Biomimetic Models to Investigate Membrane Biophysics Affecting Lipid–Protein Interaction
Frontiers in Bioengineering and Biotechnology, 2020 Biological membranes are highly dynamic in their ability to orchestrate vital mechanisms including cellular protection, organelle compartmentalization, cellular biomechanics, nutrient transport, molecular/enzymatic recognition, and membrane fusion ...
Joe Sarkis, Joe Sarkis, Véronique Vié
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Sphingomyelin and GM1 Influence Huntingtin Binding to, Disruption of, and Aggregation on Lipid Membranes [PDF]
, 2018 Huntington disease (HD) is an inherited neurodegenerative disease caused by the expansion beyond a critical threshold of a polyglutamine (polyQ) tract near the N-terminus of the huntingtin (htt) protein. Expanded polyQ promotes the formation of a variety
Campbell, Warren A. +5 more
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Site-Specific DNA-Controlled Fusion of Single Lipid Vesicles to Supported Lipid Bilayers [PDF]
Biophysical Journal, 2010 AbstractWe investigate the Ca2+‐triggered fusion of lipid vesicles site‐selectively tethered to a DNA‐modified supported lipid bilayer array, with the DNA strands designed such that hybridization occurs in a zipperlike fashion. Prior to the addition of Ca2+, which is observed to induce docking and subsequent fusion (within 200 ms), the vesicles display
Simonsson, Lisa V. +3 more
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Wicking: a rapid method for manually inserting ion channels into planar lipid bilayers. [PDF]
PLoS ONE, 2013 The planar lipid bilayer technique has a distinguished history in electrophysiology but is arguably the most technically difficult and time-consuming method in the field.
Justin A Costa +4 more
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PH regulation of amphotericin B channels activity in the bilayer lipid membrane [PDF]
, 2016 Background: Amphotericin B (AmB) is a polyene antibiotic frequently applied in the treatment of systemic fungal infections in spite of its secondary effects.
Ashrafpour, M. +2 more
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Simulation of fusion-mediated nanoemulsion interactions with model lipid bilayers [PDF]
Soft Matter, 2012 Perfluorocarbon-based nanoemulsion particles have become promising platforms for the delivery of therapeutic and diagnostic agents to specific target cells in a non-invasive manner. A "contact-facilitated" delivery mechanism has been proposed wherein the emulsifying phospholipid monolayer on the nanoemulsion surface contacts and forms a lipid complex ...
Sun-Joo, Lee +4 more
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The structural dynamics of the flavivirus fusion peptide-membrane interaction. [PDF]
PLoS ONE, 2012 Membrane fusion is a crucial step in flavivirus infections and a potential target for antiviral strategies. Lipids and proteins play cooperative roles in the fusion process, which is triggered by the acidic pH inside the endosome. This acidic environment
Ygara S Mendes +9 more
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