Results 51 to 60 of about 74,745 (203)

Atomic Force Microscope Studies of the Fusion of Floating Lipid Bilayers [PDF]

open access: yesBiophysical Journal, 2007
This study investigated the fusion of apposing floating bilayers of egg L-alpha-phosphatidylcholine (egg PC) or 1,2-dimyristoyl-sn-glycero-3-phosphocholine. Atomic force microscope measurements of fusion forces under different compression rates were acquired to reveal the energy landscape of the fusion process under varied lipid composition and ...
Abdulreda, Midhat H., Moy, Vincent T.
openaire   +2 more sources

Vesicle Fusion Observed by Content Transfer across a Tethered Lipid Bilayer [PDF]

open access: yesBiophysical Journal, 2011
Synaptic transmission is achieved by exocytosis of small, synaptic vesicles containing neurotransmitters across the plasma membrane. Here, we use a DNA-tethered freestanding bilayer as a target architecture that allows observation of content transfer of individual vesicles across the tethered planar bilayer.
Rawle, Bob   +4 more
openaire   +2 more sources

Cell-free protein expression systems in microdroplets: stabilization of interdroplet bilayers

open access: yes, 2013
Cell-free protein expression with bacterial lysates has been demonstrated to produce soluble proteins in microdroplets. However, droplet assays with expressed membrane proteins require the presence of a lipid bilayer.
Hywel Morgan   +3 more
core   +1 more source

Spontaneous Expulsion of Giant Lipid Vesicles Induced by Laser Tweezers [PDF]

open access: yes, 1996
Irradiation of a giant unilamellar lipid bilayer vesicle with a focused laser spot leads to a tense pressurized state which persists indefinitely after laser shutoff.
Bar-Ziv, Roy   +3 more
core   +3 more sources

Membrane-Bound Configuration and Lipid Perturbing Effects of Hemagglutinin Subunit 2 N-Terminus Investigated by Computer Simulations

open access: yesFrontiers in Molecular Biosciences, 2022
Hemagglutinin (HA) mediated fusion of influenza virus envelope with host lipid membrane is a critical step warrantying virus entry to the cell. Despite tremendous advances in structural biology methods, the knowledge concerning the details of HA2 subunit
Michal Michalski, Piotr Setny
doaj   +1 more source

Untargeted Lipidomics of Vesicular Stomatitis Virus-Infected Cells and Viral Particles

open access: yesViruses, 2021
The viral lifecycle is critically dependent upon host lipids. Enveloped viral entry requires fusion between viral and cellular membranes. Once an infection has occurred, viruses may rely on host lipids for replication and egress.
Katherine E. Havranek   +3 more
doaj   +1 more source

A Simple Computer Model for Liquid Lipid Bilayers

open access: yes, 2005
We present a simple coarse-grained bead-and-spring model for lipid bilayers. The system has been developed to reproduce the main (gel-liquid) transition of biological membranes on intermediate length scales of a couple of nanometres and is very efficient
Bloom   +20 more
core   +1 more source

Tuning ubiquinone position in biomimetic monolayer membranes [PDF]

open access: yes, 2017
Artificial lipid bilayers have been extensively studied as models that mimic natural membranes (biomimetic membranes). Several attempts of biomimetic membranes inserting ubiquinone (UQ) have been performed to enlighten which the position of UQ in the ...
Guaus Guerrero, Ester   +2 more
core   +2 more sources

Lipid Bilayer Vesicle Fusion: Intermediates Captured by High-Speed Microfluorescence Spectroscopy [PDF]

open access: yesBiophysical Journal, 2003
The fusion of lipid bilayers can be visualized under the fluorescence microscope, but the process is very fast and requires special techniques for its study. It is reported here that vesicle fusion is susceptible to analysis by microspectrofluorometry and that for the first time, the entire fusion process has been captured.
Lei, Guohua, MacDonald, Robert C.
openaire   +2 more sources

Membrane-spanning lipids for an uncompromised monitoring of membrane fusion and intermembrane lipid transfer

open access: yesJournal of Lipid Research, 2015
A Förster resonance energy transfer-based fusion and transfer assay was developed to study, in model membranes, protein-mediated membrane fusion and intermembrane lipid transfer of fluorescent sphingolipid analogs.
Günter Schwarzmann   +2 more
doaj   +1 more source

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