Results 61 to 70 of about 672,835 (295)
Journal of Infection and Public HealthPneumonia, a frequent and serious complication in kidney transplant recipients, is significantly increased by long-term immunosuppressive medication. This case report details a patient with lung cavitation, in which conventional microbiological methods ...
Suzan Alzeer +6 more
doaj +1 more source
Partial bisulfite conversion for unique template sequencing [PDF]
, 2018 We introduce a new protocol, mutational sequencing or muSeq, which uses sodium bisulfite to randomly deaminate unmethylated cytosines at a fixed and tunable rate.
Forcier, T. +6 more
core +1 more source
Function‐driven design of a surrogate interleukin‐2 receptor ligand
FEBS Letters, EarlyView.Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang +9 more
wiley +1 more source
Long-Reads-Based Metagenomics in Clinical Diagnosis With a Special Focus on Fungal Infections
Frontiers in Microbiology, 2022 Identification of the causative infectious agent is essential in the management of infectious diseases, with the ideal diagnostic method being rapid, accurate, and informative, while remaining cost-effective.
Minh Thuy Vi Hoang +10 more
doaj +1 more source
Inference of Markovian Properties of Molecular Sequences from NGS Data and Applications to Comparative Genomics [PDF]
, 2015 Next Generation Sequencing (NGS) technologies generate large amounts of short read data for many different organisms. The fact that NGS reads are generally short makes it challenging to assemble the reads and reconstruct the original genome sequence. For
Cannon, Charles H. +5 more
core +1 more source
Mechanisms of parasite‐mediated disruption of brain vessels
FEBS Letters, EarlyView.Parasites can affect the blood vessels of the brain, often causing serious neurological problems. This review explains how different parasites interact with and disrupt these vessels, what this means for brain health, and why these processes matter. Understanding these mechanisms may help us develop better ways to prevent or treat brain infections in ...
Leonor Loira +3 more
wiley +1 more source
Benchmarking datasets for assembly-based variant calling using high-fidelity long reads
BMC Genomics, 2023 Background Recent advances in long-read sequencing technologies have enabled accurate identification of all genetic variants in individuals or cells; this procedure is known as variant calling.
Hyunji Lee, Jun Kim, Junho Lee
doaj +1 more source
The newfound relationship between extrachromosomal DNAs and excised signal circles
FEBS Letters, EarlyView.Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley +1 more source
SMURF-seq: efficient copy number profiling on long-read sequencers
Genome Biology, 2019 We present SMURF-seq, a protocol to efficiently sequence short DNA molecules on a long-read sequencer by randomly ligating them to form long molecules. Applying SMURF-seq using the Oxford Nanopore MinION yields up to 30 fragments per read, providing an ...
Rishvanth K. Prabakar +3 more
doaj +1 more source
The application of long-read sequencing in clinical settings
Human Genomics, 2023 Long-read DNA sequencing technologies have been rapidly evolving in recent years, and their ability to assess large and complex regions of the genome makes them ideal for clinical applications in molecular diagnosis and therapy selection, thereby ...
Josephine B. Oehler +4 more
doaj +1 more source