Results 71 to 80 of about 680,559 (291)

From Pine Cones to Read Clouds: Rescaffolding the Megagenome of Sugar Pine (Pinus lambertiana). [PDF]

, 2017
We investigate the utility and scalability of new read cloud technologies to improve the draft genome assemblies of the colossal, and largely repetitive, genomes of conifers.
Crepeau, Marc W, Langley, Charles H, Stevens, Kristian A   +2 more
core   +2 more sources

Phenotypic and genotypic characterization of single circulating tumor cells in the follow‐up of high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Single circulating tumor cells (sCTCs) from high‐grade serous ovarian cancer patients were enriched, imaged, and genomically profiled using WGA and NGS at different time points during treatment. sCTCs revealed enrichment of alterations in Chromosomes 2, 7, and 12 as well as persistent or emerging oncogenic CNAs, supporting sCTC identity.
Carolin Salmon, Rui P. L. Neves, Nikolas H. Stoecklein, Sven‐Thorsten Liffers, Jens Siveke, Jan D. Kuhlmann, Pauline Wimberger, Paul Buderath, Rainer Kimmig, Sabine Kasimir‐Bauer   +9 more
wiley   +1 more source

Benchmarking datasets for assembly-based variant calling using high-fidelity long reads

BMC Genomics, 2023
Background Recent advances in long-read sequencing technologies have enabled accurate identification of all genetic variants in individuals or cells; this procedure is known as variant calling.
Hyunji Lee, Jun Kim, Junho Lee
doaj   +1 more source

Long-Reads-Based Metagenomics in Clinical Diagnosis With a Special Focus on Fungal Infections

Frontiers in Microbiology, 2022
Identification of the causative infectious agent is essential in the management of infectious diseases, with the ideal diagnostic method being rapid, accurate, and informative, while remaining cost-effective.
Minh Thuy Vi Hoang, Minh Thuy Vi Hoang, Laszlo Irinyi, Laszlo Irinyi, Laszlo Irinyi, Yiheng Hu, Benjamin Schwessinger, Wieland Meyer, Wieland Meyer, Wieland Meyer, Wieland Meyer   +10 more
doaj   +1 more source

Long-read sequence assembly of the gorilla genome [PDF]

Science, 2016
Improving on the gorilla genome Access to complete, high-quality genomes of nonhuman primates will also help us understand human biology. Gordon et al. used long-read sequencing technology to improve genome data on our close relative the gorilla.
Gordon, David, Huddleston, John, Chaisson, Mark JP, Hill, Christopher M, Kronenberg, Zev N, Munson, Katherine M, Malig, Maika, Raja, Archana, Fiddes, Ian, Hillier, LaDeana W, Dunn, Christopher, Baker, Carl, Armstrong, Joel, Diekhans, Mark, Paten, Benedict, Shendure, Jay, Wilson, Richard K, Haussler, David, Chin, Chen-Shan, Eichler, Evan E   +19 more
openaire   +4 more sources

Complex genome assembly based on long-read sequencing

Briefings in Bioinformatics, 2022
Abstract High-quality genome chromosome-scale sequences provide an important basis for genomics downstream analysis, especially the construction of haplotype-resolved and complete genomes, which plays a key role in genome annotation, mutation detection, evolutionary analysis, gene function research, comparative genomics and other aspects.
Tianjiao Zhang, Jie Zhou, Wentao Gao, Yuran Jia, Yanan Wei, Guohua Wang   +5 more
openaire   +2 more sources

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Long‐read sequencing for biodiversity analyses—A comprehensive guide

Methods in Ecology and Evolution
DNA‐based monitoring of biodiversity has revolutionised our ability to describe communities and rapidly assess anthropogenic impacts on biodiversity.
Iliana Bista, Alexandra Lino
doaj   +1 more source

LongReadSum: A fast and flexible quality control and signal summarization tool for long-read sequencing data

Computational and Structural Biotechnology Journal
While several well-established quality control (QC) tools exist for short-read sequencing data, there is a general paucity of computational tools that efficiently deliver comprehensive metrics across a wide range of long-read sequencing data formats ...
Jonathan Elliot Perdomo, Mian Umair Ahsan, Qian Liu, Li Fang, Kai Wang   +4 more
doaj   +1 more source

The application of long-read sequencing in clinical settings

Human Genomics, 2023
Long-read DNA sequencing technologies have been rapidly evolving in recent years, and their ability to assess large and complex regions of the genome makes them ideal for clinical applications in molecular diagnosis and therapy selection, thereby ...
Josephine B. Oehler, Helen Wright, Zornitza Stark, Andrew J. Mallett, Ulf Schmitz   +4 more
doaj   +1 more source