Towards reliable whole genome sequencing for outbreak preparedness and response
BMC Genomics, 2022 Background To understand the dynamics of infectious diseases, genomic epidemiology is increasingly advocated, with a need for rapid generation of genetic sequences during outbreaks for public health decision making.
David F. Nieuwenhuijse +5 more
doaj +1 more source
phasebook: haplotype-aware de novo assembly of diploid genomes from long reads
Genome Biology, 2021 Haplotype-aware diploid genome assembly is crucial in genomics, precision medicine, and many other disciplines. Long-read sequencing technologies have greatly improved genome assembly.
Xiao Luo +2 more
doaj +1 more source
enutrition reads - Ultime Result For Health
, 2020 enutrition reads Reviews There are other benefits as well to drinking more water. When weight lifting, the increased water content improves muscle repair and recovery. Think of it as opening another lane in a highway.
enutrition reads
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Long-Read Annotation: Automated Eukaryotic Genome Annotation Based on Long-Read cDNA Sequencing [PDF]
Plant Physiology, 2018 Single-molecule full-length complementary DNA (cDNA) sequencing can aid genome annotation by revealing transcript structure and alternative splice forms, yet current annotation pipelines do not incorporate such information. Here we present long-read annotation (LoReAn) software, an automated annotation pipeline utilizing short- and long-read cDNA ...
David E. Cook +5 more
openaire +2 more sources
Identifying and correcting repeat-calling errors in nanopore sequencing of telomeres
Genome Biology, 2022 Nanopore long-read sequencing is an emerging approach for studying genomes, including long repetitive elements like telomeres. Here, we report extensive basecalling induced errors at telomere repeats across nanopore datasets, sequencing platforms ...
Kar-Tong Tan +3 more
doaj +1 more source
Long-fragment targeted capture for long read sequencing of plastomes [PDF]
Applications in Plant Sciences, 2018 Third generation sequencing methods generate significantly longer reads than those produced using alternative sequencing methods. This provides increased possibilities to better study biodiversity, phylogeography and population genetics. We developed a protocol for in-solution enrichment hybridization capture of long DNA fragments applicable to ...
Kevin Bethune +11 more
openaire +8 more sources
Haplotype threading: accurate polyploid phasing from long reads
Genome Biology, 2020 Resolving genomes at haplotype level is crucial for understanding the evolutionary history of polyploid species and for designing advanced breeding strategies.
Sven D. Schrinner +8 more
doaj +1 more source
Reading Together: California Campuses and Communities Connect through America Reads [PDF]
, 1997 Reading Together provides an in-depth look at how America Reads is working in California. It describes how America Reads operates in schools and communities and offers insight into what makes for strikingly successful programs.
America Reads
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QTrim : a novel tool for the quality trimming of sequence reads generated using the Roche/454 sequencing platform [PDF]
, 2014 Background Many high throughput sequencing (HTS) approaches, such as the Roche/454 platform, produce sequences in which the quality of the sequence (as measured by a Phred-like quality scores) decreases linearly across a sequence read.
Grace P McCormack +11 more
core +1 more source
A comprehensive evaluation of alignment algorithms in the context of RNA-seq. [PDF]
, 2012 Transcriptome sequencing (RNA-Seq) overcomes limitations of previously used RNA quantification methods and provides one experimental framework for both high-throughput characterization and quantification of transcripts at the nucleotide level.
Friedel, Caroline C. +8 more
core +2 more sources