Results 61 to 70 of about 327,413 (252)
Haplotype and Repeat Separation in Long Reads [PDF]
, 2017 Abstract Resolving the correct structure and succession of highly similar sequence stretches is one of the main open problems in genome assembly. For non haploid genomes this includes determining the sequences of the different haplotypes. For all but the smallest genomes it also involves separating different repeat instances.
openaire +2 more sources
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
Long‐read genotyping with SLANG (Simple Long‐read loci Assembly of Nanopore data for Genotyping)
Applications in Plant Sciences, 2022 AbstractPremiseMost phylogenomic library preparation methods and bioinformatic analysis tools in restriction site–associated DNA sequencing (RADseq)/genotyping‐by‐sequencing (GBS) studies are designed for use with Illumina data. The lack of alternative bioinformatic pipelines hinders the exploration of long‐read multi‐locus data from other sequencing ...
Marco Dorfner +3 more
openaire +4 more sources
FEBS Letters, EarlyView.In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai +9 more
wiley +1 more source
HALC: High throughput algorithm for long read error correction
BMC Bioinformatics, 2017 Background The third generation PacBio SMRT long reads can effectively address the read length issue of the second generation sequencing technology, but contain approximately 15% sequencing errors.
Ergude Bao, Lingxiao Lan
doaj +1 more source
PacBio Long Reads Improve Metagenomic Assemblies, Gene Catalogs, and Genome Binning
Frontiers in Genetics, 2020 PacBio long reads sequencing presents several potential advantages for DNA assembly, including being able to provide more complete gene profiling of metagenomic samples.
Haiying Xie +7 more
doaj +1 more source
Frontiers in Marine Science, 2023 Metagenomic sequencing facilitates large-scale constitutional analysis and functional characterization of complex microbial communities without cultivation.
Mengyang Xu +15 more
doaj +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
Evaluation and Validation of Assembling Corrected PacBio Long Reads for Microbial Genome Completion via Hybrid Approaches. [PDF]
PLoS ONE, 2015 Despite the ever-increasing output of next-generation sequencing data along with developing assemblers, dozens to hundreds of gaps still exist in de novo microbial assemblies due to uneven coverage and large genomic repeats.
Hsin-Hung Lin, Yu-Chieh Liao
doaj +1 more source
Genome and Evolutionary Analysis of Nosema ceranae: A Microsporidian Parasite of Honey Bees
Frontiers in Microbiology, 2021 Microsporidia comprise a phylum of single cell, intracellular parasites and represent the earliest diverging branch in the fungal kingdom. The microsporidian parasite Nosema ceranae primarily infects honey bee gut epithelial cells, leading to impaired ...
Qiang Huang +10 more
doaj +1 more source