Results 71 to 80 of about 6,745,034 (280)
HALC: High throughput algorithm for long read error correction
BMC Bioinformatics, 2017 Background The third generation PacBio SMRT long reads can effectively address the read length issue of the second generation sequencing technology, but contain approximately 15% sequencing errors.
Ergude Bao, Lingxiao Lan
doaj +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
PacBio Long Reads Improve Metagenomic Assemblies, Gene Catalogs, and Genome Binning
Frontiers in Genetics, 2020 PacBio long reads sequencing presents several potential advantages for DNA assembly, including being able to provide more complete gene profiling of metagenomic samples.
Haiying Xie +7 more
doaj +1 more source
Frontiers in Marine Science, 2023 Metagenomic sequencing facilitates large-scale constitutional analysis and functional characterization of complex microbial communities without cultivation.
Mengyang Xu +15 more
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ISMB 2020 Tutorial Long-Reads RNA Training
, 2021 Training materials for long-reads RNA data ...
Yedomon Ange Bovys Zoclanclounon
core +1 more source
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
FEBS Letters, EarlyView.GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Long‐read genotyping with SLANG (Simple Long‐read loci Assembly of Nanopore data for Genotyping)
Applications in Plant Sciences, 2022 AbstractPremiseMost phylogenomic library preparation methods and bioinformatic analysis tools in restriction site–associated DNA sequencing (RADseq)/genotyping‐by‐sequencing (GBS) studies are designed for use with Illumina data. The lack of alternative bioinformatic pipelines hinders the exploration of long‐read multi‐locus data from other sequencing ...
Marco Dorfner +3 more
openaire +3 more sources
Evaluation and Validation of Assembling Corrected PacBio Long Reads for Microbial Genome Completion via Hybrid Approaches. [PDF]
PLoS ONE, 2015 Despite the ever-increasing output of next-generation sequencing data along with developing assemblers, dozens to hundreds of gaps still exist in de novo microbial assemblies due to uneven coverage and large genomic repeats.
Hsin-Hung Lin, Yu-Chieh Liao
doaj +1 more source
Genome and Evolutionary Analysis of Nosema ceranae: A Microsporidian Parasite of Honey Bees
Frontiers in Microbiology, 2021 Microsporidia comprise a phylum of single cell, intracellular parasites and represent the earliest diverging branch in the fungal kingdom. The microsporidian parasite Nosema ceranae primarily infects honey bee gut epithelial cells, leading to impaired ...
Qiang Huang +10 more
doaj +1 more source
Epigenetic reprogramming of lineage switching in cancer
FEBS Letters, EarlyView.Cancer cells rarely commit to a single identity. Epigenetic mechanisms and tumor microenvironment cues push epithelial cells toward flexible, hybrid states that can shift into mesenchymal, neuroendocrine, or stem‐like fates, driving metastasis, drug resistance, and tumor heterogeneity. Targeting the epigenetic regulators behind these transitions, using
Ezgi Boyvatlı +4 more
wiley +1 more source