Results 21 to 30 of about 6,745,034 (280)
Short paired-end reads trump long single-end reads for expression analysis
BMC Bioinformatics, 2020 Background Typical experimental design advice for expression analyses using RNA-seq generally assumes that single-end reads provide robust gene-level expression estimates in a cost-effective manner, and that the additional benefits obtained from paired ...
Adam H. Freedman +2 more
doaj +1 more source
A comprehensive investigation of metagenome assembly by linked-read sequencing
Microbiome, 2020 Background The human microbiota are complex systems with important roles in our physiological activities and diseases. Sequencing the microbial genomes in the microbiota can help in our interpretation of their activities.
Lu Zhang +8 more
doaj +1 more source
Haplotype-Phased Synthetic Long Reads from Short-Read Sequencing. [PDF]
PLoS ONE, 2016 Next-generation DNA sequencing has revolutionized the study of biology. However, the short read lengths of the dominant instruments complicate assembly of complex genomes and haplotype phasing of mixtures of similar sequences.
James A Stapleton +12 more
doaj +1 more source
, 2021 Assembled and polished minION long-reads from human oral cavity samples processed using three HMW DNA extraction methods (M4, M5, and M6, each with two replicates) described in the following study:https://doi.org/10.1101/2021.03.03.433801The data pack ...
Florian Trigodet (10219307) +2 more
core +1 more source
Nanopore long-read sequencing of circRNAs [PDF]
Methods, 2021 Circular RNA (circRNA) is a group of highly stable RNA molecules with suggested roles in development and disease. They derive from linear pre-mRNAs when a 5'-splice site splices back to an upstream 3'-splice site in a process termed back-splicing. Most circRNAs are multi-exonic and may contain several thousand nucleotides.
Rahimi, Karim +3 more
openaire +3 more sources
Long-read amplicon denoising [PDF]
Nucleic Acids Research, 2019 Abstract Long-read next-generation amplicon sequencing shows promise for studying complete genes or genomes from complex and diverse populations. Current long-read sequencing technologies have challenging error profiles, hindering data processing and incorporation into downstream analyses.
Venkatesh Kumar +12 more
openaire +2 more sources
viralFlye: assembling viruses and identifying their hosts from long-read metagenomics data
Genome Biology, 2022 Although the use of long-read sequencing improves the contiguity of assembled viral genomes compared to short-read methods, assembling complex viral communities remains an open problem.
Dmitry Antipov +3 more
doaj +1 more source
N1 single-cell barcoded RNA-seq ONT long-reads
, 2023 The dataset is extracted from an RNA human sample. The RNA molecules have 10x Genomics single-cell barcodes + UMI + Illumina read adapters added to them. Then the ONT long-read adatper is added.
Baraa Orabi (12507379)
core +1 more source
Comparison and benchmark of structural variants detected from long read and long-read assembly
Briefings in Bioinformatics, 2023 Abstract Structural variant (SV) detection is essential for genomic studies, and long-read sequencing technologies have advanced our capacity to detect SVs directly from read or de novo assembly, also known as read-based and assembly-based strategy. However, to date, no independent studies have compared and benchmarked the two strategies.
Jiadong Lin +4 more
openaire +4 more sources
BMC Genomics, 2021 Background SNP arrays, short- and long-read genome sequencing are genome-wide high-throughput technologies that may be used to assay copy number variants (CNVs) in a personal genome.
Ksenia Lavrichenko +2 more
doaj +1 more source