Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination
Malaria Journal, 2019 Background Malaria elimination requires diagnostic methods able to detect parasite levels well below what is currently possible with microscopy and rapid diagnostic tests.
Abu Naser Mohon +4 more
doaj +1 more source
Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time [PDF]
, 2018 Real-time, isothermal, digital nucleic acid amplification is emerging as an attractive approach for a multitude of applications including diagnostics, mechanistic studies, and assay optimization.
Ismagilov, Rustem F. +3 more
core +3 more sources
Peroxidase‐Mimicking Nanozymes for Rapid Detection of Infectious Diseases
Advanced Science, EarlyView.Peroxidase‐mimicking nanozymes (PMNs) have emerged as robust and versatile materials for rapid infectious disease diagnostics. This review highlights the rational design and controlled synthesis of PMNs, summarizes key biomarkers relevant to infectious diseases, examines their integration into diverse rapid detection platforms, and highlights ...
Shikuan Shao +5 more
wiley +1 more source
Diagnostic performance of a novel loop-mediated isothermal amplification (LAMP) assay targeting the apicoplast genome for malaria diagnosis in a field setting in sub-Saharan Africa. [PDF]
, 2015 BACKGROUND: New diagnostic tools to detect reliably and rapidly asymptomatic and low-density malaria infections are needed as their treatment could interrupt transmission.
D'Alessandro, Umberto +5 more
core +1 more source
Advanced Science, EarlyView.The TopCas platform utilizes an engineered DNA‐RNA chimeric circular crRNA to establish topology‐gated control over CRISPR‐Cas12a activity, enabling target‐triggered self‐catalytic amplification (preamplification‐free). This system seamlessly integrates highly sensitive molecular diagnostics with conditionally controlled gene editing, demonstrating ...
Shun Zhang +7 more
wiley +1 more source
The development of loop-mediated isothermal amplification targeting alpha-tubulin DNA for the rapid detection of Plasmodium ...
Byoung-kuk Na +10 more
core
Advances in Microfluidics and Lab-on-a-Chip Technologies
, 2017 Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense.
Abate +161 more
core +1 more source
Detection of Pseudomonas fluorescens from broth, water and infected tissues by loop-mediated isothermal amplification (LAMP) method [PDF]
, 2015 Loop mediated isothermal amplification is rapid, highly sensitive and specifically developed method for detection of bacterial infections. AprX gene for alkaline metalloprotease of Pseudomonas fluorescens was used to design four primers and loop mediated
Duhan, JS, Gahlawat, SK, Saharan, P
core +2 more sources
Development of a recombinase polymerase amplification lateral flow assay for the detection of active Trypanosoma evansi infections [PDF]
, 2020 Author summary Neglected tropical diseases (NTDs) affecting humans and/or domestic animals severely impair the socio-economic development of endemic areas. One of these diseases, animal trypanosomosis, affects livestock and is caused by the parasites of
Goossens, Julie +5 more
core +1 more source
Advanced Science, EarlyView.A novel transcriptional module involving CsYABBY3 and CsAS1 is identified to regulate cannabinoid biosynthesis and trichome development. These factors mutually activate each other and form a protein complex via a conserved residue, acting synergistically to amplify metabolic flux through a coordinate feed‐forward mechanism.
Xuewen Zhu +18 more
wiley +1 more source