Results 121 to 130 of about 52,140 (330)

Detection of Pathogen-Specific Antibodies by Loop-Mediated Isothermal Amplification [PDF]

Clinical and Vaccine Immunology, 2015
ABSTRACTLoop-mediated isothermal amplification (LAMP) is a method for enzymatically replicating DNA that has great utility for clinical diagnosis at the point of care (POC), given its high sensitivity, specificity, speed, and technical requirements (isothermal conditions).
Burbulis, Ian, Yamaguchi, Kumiko, Nikolskaia, Olga, Prigge, Sean, Magez, Stefan, Bisser, Sylvie, Reller, Megan, Grab, Dennis   +7 more
openaire   +4 more sources

TBK1 Induces the Formation of Optineurin Filaments That Condensate with Polyubiquitin and LC3 for Cargo Sequestration

Advanced Science, EarlyView.
Phosphorylation of Optineurin by TBK1 induces the formation of filaments that condensate upon binding to linear polyubiquitin. Membrane‐anchored LC3 partitions into these condensates, suggesting that phase separation of filamentous Optineurin with ubiquitylated cargo promotes the sequestration of cargo and its subsequent alignment with LC3‐positive ...
Maria G. Herrera, Lena Kühn, Lisa Jungbluth, Verian Bader, Laura J. Krause, David Kartte, Elias Adriaenssens, Sascha Martens, Jörg Tatzelt, Carsten Sachse, Konstanze F. Winklhofer   +10 more
wiley   +1 more source

Utility of blood as the clinical specimen for the diagnosis of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification and real-time polymerase chain reaction assays based on REP-529 sequence and B1 gene [PDF]

, 2022
Bahman Rahimi Esboei, Shirzad Fallahi, Mohammad Zarei, Bahram Kazemi, Mehdi Mohebali, Saeedeh Shojaee, Parisa Mousavi, Aref Teimouri, Raziyeh Mahmoudzadeh, Mirataollah Salabati, Hossein Keshavarz Valian   +10 more
openalex   +1 more source

Precise Regulation of Membrane Proteins: From Physical Technology to Biomolecular Strategy

Advanced Science, EarlyView.
This review summarizes the emerging strategies for the precise regulation of membrane proteins using physical stimuli and biomolecule‐based tools. These methods provide new insights into cell regulation and offer promising directions for future disease treatment.
Xiu Zhao, Qingqing Zhou, Mengli Li, Wen Zou, Zhenzhong Zhang, Jinjin Shi, Junjie Liu   +6 more
wiley   +1 more source

A stoichiometric and pseudo kinetic model of loop mediated isothermal amplification

Computational and Structural Biotechnology Journal, 2020
Loop mediated isothermal amplification (LAMP) is one of the most popular isothermal DNA amplification techniques for research and commercial applications, enabling amplification of both DNA and RNA (with the assistance of reverse transcriptase). The LAMP
Navjot Kaur, Nikhil Thota, Bhushan J. Toley   +2 more
doaj   +1 more source

Detection of Pseudomonas fluorescens from broth, water and infected tissues by loop-mediated isothermal amplification (LAMP) method [PDF]

, 2015
Loop mediated isothermal amplification is rapid, highly sensitive and specifically developed method for detection of bacterial infections. AprX gene for alkaline metalloprotease of Pseudomonas fluorescens was used to design four primers and loop mediated
Duhan, JS, Gahlawat, SK, Saharan, P
core   +2 more sources

Clinical Performance of Reverse Transcription Loop Mediated Isothermal Amplification COVID-19 Assay on Gold- nanoparticle-modified Screen-printed Carbon Electrode Using Differential Pulse Voltammetry [PDF]

, 2023
Munirah Zulhairee, Rozainanee Mohd Zain, Adibah Che Mohamad Nor, Nur Fadhilah Jafar, Mohd Fairulnizal Md Noh, Mohd Shihabuddin Ahmad Noorden, Anis Nurashikin Nordin, Rosminazuin Ab Rahim, Teddy Surya Gunawan, Ying-Chin Lim, Yusairie Mohd, Nur Asyiqin Azman, Zainiharyati Mohd Zain   +12 more
openalex   +1 more source

Targeting Peptostreptococcus anaerobius with an Iron‐Based Nanozyme Reverses Ferroptosis Resistance and Enhances Antitumor Immunity in Colorectal Cancer

Advanced Science, EarlyView.
An iron‐based nanozyme selectively eliminates intratumoral P. anaerobius while catalytically generating ROS to induce ferroptosis, synergistically suppressing colorectal cancer growth and activating anti‐tumor immunity through immunogenic cell death. ABSTRACT The intratumoral microbiota is a critical determinant of therapeutic outcomes in colorectal ...
Yinghao Cao, Jun Wang, Hanwenchen Wang, Xiang Sun, Fang Fang, Shiying Li, Jianping Liu, Changrong Shi, Pengyuan Qi, Jianhua Zou, Xiaoyuan Chen, Kailin Cai   +11 more
wiley   +1 more source

Frontiers of parasitology research in the People's Republic of China : infection, diagnosis, protection and surveillance [PDF]

, 2012
: Control and eventual elimination of human parasitic diseases in the People's Republic of China (P.R. China) requires novel approaches, particularly in the areas of diagnostics, mathematical modelling, monitoring, evaluation, surveillance and public ...
Bergquist, R., Chen, J. H., Chen, J. X., Tanner, M., Utzinger, J., Wang, H., Zhou, X. N.   +6 more
core   +2 more sources

SMart Nanoparticle–Hydrogel Hybrid System for Synergistic Eradication of Infection and Promotion of Wound Healing

Advanced Science, EarlyView.
The integrated system, comprising bacterial‐targeted photothermal nanoparticles (Fe2O3‐CS@RBP) and multifunctional hydrogels (PAOC‐3@BA) modules, achieves rapid wound healing within one week. This work introduces a smart therapeutic platform that integrates bacterial targeting, self‐regulating photothermal therapy, tissue adhesion, and stimulus ...
Hongping Wan, Huirong Tan, Xinghong Zhao
wiley   +1 more source