Results 131 to 140 of about 52,140 (330)

Loop-Mediated Isothermal Amplification Targeting Actin DNA of Trichomonas vaginalis [PDF]

, 2016
Trichomoniasis caused by Trichomonas vaginalis is a common sexually transmitted disease. Its association with several health problems, including preterm birth, pelvic inflammatory disease, cervical cancer, and transmission of human immunodeficiency virus,
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core   +1 more source

Droplet Digital CRISPR for Nucleic Acid Detection

Advanced Science, EarlyView.
This review outlines recent advances in droplet digital CRISPR technology for nucleic acid detection, combining CRISPR specificity with droplet digital‐based absolute quantification. It summarizes core principles, amplification‐assisted and amplification‐free strategies, and representative DNA and RNA biomarker applications.
Yang Zhang, Roy S. K. Walker, Anwar Sunna, Tracie J. Barber, Ming Li   +4 more
wiley   +1 more source

Detection of Clostridium perfringens alpha toxin gene in lambs by loop mediated isothermal amplification [PDF]

Veterinary World, 2016
Aim: The loop mediated isothermal amplification (LAMP) was standardized for rapid detection of Clostridium perfringens. Materials and Methods: A total of 120 fecal samples were collected from enterotoxemia suspected lambs were used for screening of C ...
B. Radhika , N. Vinod Kumar , D. Sreenivasulu   +2 more
doaj   +1 more source

Advances in Microfluidics and Lab-on-a-Chip Technologies

, 2017
Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense.
Abate, Adley, Aebersold, Ahlford, Almassian, Almoguera, Asiello, Athamanolap, Baker, Becker, Blazej, Brehm-Stecher, Burger, Business-Wire, Byrnes, Cai, Cao, Chang, Chao, Chatterjee, Chin, Chin, Coupland, Craw, Crews, Crews, Czilwik, Derveaux, DeVoe, Dharmasiri, Dodsworth, Domon, Duffy, Erlich, Farrar, Fredrickson, Fu, Gagnon, Gao, Gonzalez de Castro, Grover, Hadd, Han, Hanyoup, Hardouin, Hauck, Hayden, He, He, He, Hindson, Ho, Hsieh, Huang, Hughes, Jacobs, Jansson, Jarvius, Jayamohan, Jayamohan, Jenkins, Jha, Ji, Jin, Jin, Kalra, Karimi, Karns, Khandurina, Kim, Kim, Kim, Kim, Kim, Klein, Klostranec, Konry, Kuroda, Kwasny, Kwasny, Kühnemund, Küster, Lagally, Lapierre, Lazar, Lazar, Lee, Lee, Lee, Li, Liu, Lochovsky, Macosko, Mahmoudian, Mani, Manz, Mao, Mariella, Melin, Mery, Metzker, Mezger, Mitchell, Mitchell, Neuzil, Ng, Northen, Oblath, Oxford Nanopore, Paegel, Paegel, Paliwal, Park, Pekin, Pennisi, Perez-Toralla, Pješčić, Pješčić, Pješčić, Prakash, Priye, Priye, Quick, Ro, Romanov, Safavieh, Sainiemi, Samuel, Sanvicens, Sato, Sayad, Schoenitz, Schuler, Shamsi, Shih, Song, Srbek, Stumpf, Sun, Sundberg, Tanaka, Teh, Tehranirokh, Thompson, Thuy, Tian, Tröger, Unger, Vergauwe, Volpatti, Weisenberger, Wheeler, Whitesides, Woolley, Xie, Xu, Xue, Yetisen, Yin, Zhang, Zhang, Zhu   +161 more
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Rapid detection of COVID-19 coronavirus using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform

Clinical Chemistry, 2020
1 Applied Biology Laboratory, Shenyang University of Chemical Technology, 110142, Shenyang, China 2 Key Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinformatics and Molecular-imaging, Center for Artificial ...
Lin Yu, Shanshan Wu, Xiaowen Hao, Xue Dong, L. Mao, V. Pelechano, Wei-Hua Chen, Xiushan Yin   +7 more
semanticscholar   +1 more source

Diagnosis of Protozoa by Loop-Mediated Isothermal Amplification: (LAMP)

Turkish Journal of Parasitology, 2010
Plasmodium, Isospora, Toxoplasma, Babesia and Cryptosporidium are parasites which have a significant role in human health. The location in which the protozoon settles in the body, the way that it leaves the host, and the sample which is send from the clinic are the elements of the general methods used in the diagnosis of the ailments caused from ...
Zeynep, Kolören, Cumhur, Avşar, Zülal Atlı, Şekeroğlu   +2 more
openaire   +2 more sources

MARPLE: A Proximity‐Triggered CRISPR‐Cas13 Platform for Ultrasensitive Antibody Detection

Advanced Science, EarlyView.
MARPLE is a modular CRISPR‐Cas13 immunoassay that uses antibody‐induced proximity to trigger a toehold‐exchange reaction, releasing an RNA activator for Cas13. This one‐pot, isothermal architecture enables ultrasensitive (femtomolar) and highly specific detection of diverse antibodies, including therapeutic, pathogen‐specific, and cancer‐associated ...
Elena Spezzani, Luca Capelli, Denise Di Lena, Alejandro Chamorro‐Garcia, Rudy Ippodrino, Alessandro Porchetta, Alessandro Bertucci   +6 more
wiley   +1 more source

Transforming RNA Degradation into Accurate RNA Detection: RNase I–Assisted Rolling Circle Amplification for Clinical Samples

Angewandte Chemie, EarlyView.
Here, we transform this otherwise destructive enzymatic activity into a powerful diagnostic advantage through an RNase I–assisted rolling circle amplification (RI‐RCA) strategy. By integrating controlled RNase I–mediated RNA digestion with circular DNA templates, this approach enables direct and highly sensitive detection of target RNA sequences. Using
Amal Mathai, Cheng‐Yu Lee, Zijie Zhang, Jimmy Gu, John D. Brennan, Yingfu Li   +5 more
wiley   +2 more sources

Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination

Malaria Journal, 2019
Background Malaria elimination requires diagnostic methods able to detect parasite levels well below what is currently possible with microscopy and rapid diagnostic tests.
Abu Naser Mohon, Sisay Getie, Nusrat Jahan, Mohammad Shafiul Alam, Dylan R. Pillai   +4 more
doaj   +1 more source

Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay to the Presence of Urea [PDF]

A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤1.8 M, compared with a PCR assay that was functional at ...
Behzadbehbahani, Chaudhry, Chernesky, Francois, G. Hobbs, Geraats-Peters, H. B. Smalley, Hedman, Kaneko, Khan, L. Gillies, Luijt, Nakao, Newman, Njiru, Notomi, P. A. Burke, T. Edwards, Tomita, World Health Organization   +19 more
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