Results 131 to 140 of about 35,344 (268)

Polymerase-endonuclease amplification reaction for large-scale enzymatic production of antisense oligonucleotide [PDF]

, 2009
Synthetic oligonucleotides are contaminated with highly homologous failure sequences. Oligonucleotide synthesis is difficult to scale up because it requires expensive equipments, hazardous chemicals, and tedious purification process.
Deming Gou, Xiaolong Wang
core   +1 more source

Phase separation enables dual ionic and thermal transport in solid‐state cathodes

InfoMat, EarlyView.
We design a phase‐separated ionogel composite cathode (PS cathode) with a bicontinuous network formed through ionic liquid‐polymer phase separation. Simulations and experiments show that this architecture couples efficient ionic conduction with enhanced thermal transport, thereby suppressing polarization and hotspot formation.
Guangzeng Cheng, Yonghui Wang, Ziwei Lu, Jing Shi, Jingwei Chen, Weiqian Tian, Yue Zhu, Danqi He, Jingyi Wu, Huanlei Wang   +9 more
wiley   +1 more source

Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of Actinobacillus pleuropneumoniae based the dsbE-like gene

Pesquisa Veterinária Brasileira, 2012
This paper reports on the development and validation of a loop-mediated isothermal amplification assay (LAMP) for the rapid and specific detection of Actinobacillus pleuropneumoniae (A. pleuropneumoniae).
Hongwei Ji, Haitao Li, Ling Zhu, Hui Zhang, Yin Wang, Zhicai Zuo, Wanzhu Guo, Zhiwen Xu   +7 more
doaj   +1 more source

Betulin Protects Against Cardiac Hypertrophy by Improving AMPK/Nrf2‐Dependent Mitochondrial Function

Phytotherapy Research, EarlyView.
Betulin effectively mitigates pressure overload‐induced cardiac hypertrophy in both Ang II‐infused and TAC‐operated mice. Mechanistically, betulin activated AMPK phosphorylation, promoted Nrf2 nuclear translocation, and upregulated antioxidant genes (HO‐1 and NQO‐1), thereby restoring mitochondrial function in cardiomyocytes.
Bei Zheng, Mingyang He, Haiying Wu, Tianjie Zhang, Yuxia Jiang, Chuanwei Xin, Meiling Zhang, Jiaqi He, Lulu Zheng   +8 more
wiley   +1 more source

Detection of Sugarcane yellow leaf virus by a novel reverse transcription loop-mediated isothermal amplification method from three sugarcane production regions in Kenya [PDF]

, 2013
Sugarcane yellow leaf is a disease caused by Sugarcane yellow leaf virus (SCYLV). It is a major emerging disease of sugarcane that has been reported worldwide the last two decades.
Amata, Ruth Lodenyi, Fernandez, Emmanuel, Roumagnac, Philippe   +2 more
core  

High Sensitivity Cardiac Troponin I Detection via MP‐Locked Aptamer and Multimeric DNAzyme‐Coupled Hyperbranched Hybridization Chain Reaction

Small, EarlyView.
We report a magnetic particle anchored locked aptamer platform integrated with hyperbranched HCR and DNAzyme amplification for enzyme‐free, ultrasensitive detection of cardiac troponin I. The assay achieves a 0.25 ng/L detection limit within 25 minutes and enables cross‐species MI detection, with machine learning classification reaching 90% accuracy ...
Sayantan Tripathy, Sahil Sharma, Ng Ka Wai, Siddhant Jaitpal, Anthony Dongchau, Saugat Bhattacharyya, Sonya R. Wesselowski, Ashley B. Saunders, Gerard L. Coté, Samuel B. Mabbott   +9 more
wiley   +1 more source

Mesoporous Silica Nanoparticles‐Based Formulations for Enhanced Oral Delivery of Peptide Drugs: A Case Study on Insulin

Small, EarlyView.
This work explores the tailored functionalization of mesoporous silica nanoparticles (MSN) to improve the oral delivery of insulin, as a case study. Insulin confinement within silica mesopores increased drug solubility, and co‐formulation with succinylated β‐lactoglobulin yielded pH‐responsive tablets that limited gastric release and proteolysis, while
Claudia Iriarte‐Mesa, Estelle Juère, Andrea Bileck, Thomas Kremsmayr, Michael L. Goodson, Allison Ehrlich, Adnan Hodžić, Martin Kunert, Christopher Gerner, Hanspeter Kählig, Doris Marko, Markus Muttenthaler, David Berry, Giorgia Del Favero, Freddy Kleitz   +14 more
wiley   +1 more source

Multiplex visual nucleic acid detection via isothermal cascaded transcription of fluorescent RNA aptamers

VIEW, EarlyView.
(1) Existing multiplex nucleic acid detection struggles to combine high sensitivity, visual readout, and instrument‐free use, which is vital for rapid infection and antibiotic resistance diagnosis. (2) We developed MAGIC, integrating isothermal cascaded amplification and fluorescent RNA aptamer transcription, achieving 10 aM sensitivity, 100% accuracy ...
Yu Tang, Na Luo, Binbin Xie, Jie Luo, Yaling Yuan, Junjie Fan, Zhiyong Liu, Haiyan Gao, Ming Chen, Kai Chang   +9 more
wiley   +1 more source

Application of a novel molecular diagnostic method to examine the spatio‐temporal trends of Carcelia iliaca, a larval parasitoid of oak processionary moth (Thaumetopoea processionea)

Agricultural and Forest Entomology, EarlyView.
The oak processionary moth is an invasive pest in the United Kingdom that threatens biodiversity and public health, with rising control costs highlighting the need for sustainable management alternatives Conservation biocontrol using the parasitoid fly Carcelia iliaca is promising but key biological data gaps hinder evidence‐based policy development. A
Kyle Alexander Miller, Darren Mark Evans, Neil Boonham, Andrew Hoppit, Jake Morris, James John Neil Kitson   +5 more
wiley   +1 more source

Targeting protein–protein interactions with reversible covalent modalities: Non‐cysteine chemistries

British Journal of Pharmacology, EarlyView.
Abstract Protein–protein interactions (PPIs) are central to diverse cellular functions, and represent a rapidly expanding class of therapeutic targets. Advancements in covalent drug design have enabled small‐molecule drugs to overcome challenges associated with engaging these targets, such as limited durations of action and difficult‐to‐drug (expansive,
Ruchira Basu, Steven Fletcher
wiley   +1 more source