Results 141 to 150 of about 35,344 (268)

Development of PCR, LAMP and HRM‐Based Molecular Species Diagnostic Tools for Identifying Four Agricultural Pests in the Genus Helicoverpa (Lepidoptera: Noctuidae)

Journal of Applied Entomology, EarlyView.
ABSTRACT Within the subfamily Heliothinae, the genus Helicoverpa comprises several highly destructive agricultural pests, including H. armigera, H. assulta, H. punctigera and H. zea. These species cause significant global crop losses due to their wide host range, long‐distance migratory behaviour, and potential for interspecific hybridization.
Minseop Noh, Murtaza Khan, Minyoung Choi, Andreas Zwick, Juil Kim   +4 more
wiley   +1 more source

Loop-Mediated Isothermal Amplification for Diagnosis of Zoonotic Malaria

The American Journal of Tropical Medicine and Hygiene
Zoonotic malaria, caused by Plasmodium knowlesi, Plasmodium cynomolgi, Plasmodium coatneyi, and Plasmodium inui, is a significant global health concern. The gold standard microscopy, while widely used for malaria diagnosis, faces limitations in differentiating between malaria species.
Lai, Meng Yee, Sohairi, Ainaa Nadrah, Zen, Lee Phone Youth, Abdullah, Mohd Lutfi, Lau, Yee Ling   +4 more
openaire   +2 more sources

A multiplex assay to detect mosquito species, bloodmeal host source and Plasmodium in malaria vectors using Nanopore amplicon sequencing

Medical and Veterinary Entomology, EarlyView.
Insectary‐reared mosquitoes were simultaneously assessed for species identification, bloodmeal source analysis and Plasmodium detection, using a single multiplexed sequencing run on the MinION sequencing platform. The method was applied to 150 insectary‐reared mosquitoes, representing nine species blood‐fed with five vertebrate hosts and 40 samples for
E. Abby Rogers, Dieunel Derilus, Christopher Sandi, Lisa Reimer, Audrey Lenhart, Lucy Mackenzie Impoinvil   +5 more
wiley   +1 more source

Deciphering Cold Stress Resilience: Multiomics Insights in Contrasting Wheat Genotypes From the Western Himalayas

Plant Biotechnology Journal, EarlyView.
ABSTRACT Cold stress threatens wheat productivity, particularly in regions with extreme climatic conditions. To elucidate the molecular mechanisms underlying wheat's response to cold stress, we performed a multiomics analysis integrating lipidomics, transcriptomics, proteomics and metabolomics.
Sofora Jan, Farkhandah Jan, Mukesh Rathore, Yogita Singh, Prexha Kapoor, Palak Chaturvedi, Arindam Ghatak, Palakurthi Ramesh, Upendra Kumar, Manoj Prasad, Sundeep Kumar, Sachin Rustgi, Wolfram Weckwerth, Sanjay Kalia, Rajeev Kumar Varshney, Reyazul Rouf Mir   +15 more
wiley   +1 more source

Research progress on application of novel isothermal amplification technology in waterborne pathogens detection

环境与职业医学
Water resources are crucial for environmental protection and the health of humans, plants, and animals. Contamination of water by pathogens such as bacteria, viruses, and protozoa can lead to outbreaks of various water-related infectious diseases, posing
Fubin HUANG, Eryi SHU, Hongliang FAN
doaj   +1 more source

Viable but Nonculturable State, a Survival Strategy for Salmonella in Aquatic Environments

Water and Environment Journal, EarlyView.
Transition of Salmonella from a culturable state to a viable but nonculturable state under various environmental stresses and current methods for detecting viable Salmonella in environmental samples. ABSTRACT In the relentless battle for survival, Salmonella has evolved mechanisms to withstand harsh conditions such as extreme temperatures, fluctuating ...
Sanelisiwe Thinasonke Duze, Musa Marimani, Katlego Mamabolo, Mrudula Patel   +3 more
wiley   +1 more source

Rapid detection of Mycoplasma pneumonia by Loop mediated isothermal amplification (LAMP)

مجله دانشگاه علوم پزشکی گرگان, 2015
Background and Objective: Mycoplasma pneumoniae bacteria, is one of the most important factor in causing of respiratory infections. Serological and molecular detection methods have their own limitation.
Davudi-Asl F, Shahhosseiny MH, Keshavarz F   +2 more
doaj  

Enhanced heterogeneity of rpoB in Mycobacterium tuberculosis found at low pH.

, 2009
OBJECTIVES: The aim of this study was to gain an insight into the molecular mechanisms of the evolution of rifampicin resistance in response to controlled changes in the environment.
Allnutt, Jon, Arnold, Catherine, Bacon, Joanna, Bose, Alpana, Gillespie, Stephen H, Hatch, Kim A, Jenkins, Claire, McHugh, Timothy D, O'Sullivan, Denise M   +8 more
core   +1 more source

Structure mirroring function: What's the ‘matter’ with the funny current?

The Journal of Physiology, EarlyView.
Abstract figure legend The ‘funny’ (If) current of cardiac pacemaker cells has been first identified in the late 1970s as a major mechanism in the generation and control of cardiac pacemaking. Decades of studies have since described the properties of the funny current and of its molecular components, HCN channels, in the heart and brain, providing the ...
Andrea Saponaro, Dario DiFrancesco
wiley   +1 more source

Smartphone-based pathogen diagnosis in urinary sepsis patients. [PDF]

, 2018
BackgroundThere is an urgent need for rapid, sensitive, and affordable diagnostics for microbial infections at the point-of-care. Although a number of innovative systems have been reported that transform mobile phones into potential diagnostic tools, the
Barnes, Lucien, Choe, Jane, Fitzgibbons, Lynn N, Fox, Gary N, Fried, Jeffrey C, Heithoff, Douglas M, Mahan, Michael J, Mahan, Scott P, Marth, Jamey D, Soh, H Tom, Zambrano, Andrea   +10 more
core