Making Molecular Diagnostics Faster
ABSTRACT Background Over the past 40 years, molecular diagnostic methods have evolved from multi‐step, time‐consuming protocols towards either rapid targeted tests or expansive, massively parallel testing. Aims Here we consider the speed limits of targeted molecular diagnostics, considering the three sequential required steps: nucleic acid preparation,
Carl T. Wittwer +5 more
wiley +1 more source
Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Bacterial Bovine Mastitis: A Systematic Review. [PDF]
Jember TF +3 more
europepmc +1 more source
Abstract Royal Decree 1021/2022, which regulates certain hygiene requirements for the production and marketing of foodstuffs in retail establishments, establishes that, in retail establishments, food made with raw egg subjected to heat treatment where a temperature equal to or greater than 70°C is reached for 2 seconds in the centre of the product or ...
Antonio Valero Díaz +6 more
wiley +1 more source
Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of High-Burden Viral Diseases in Resource-Limited Countries. [PDF]
Vasconcelos-Martins AC +4 more
europepmc +1 more source
Targeting Expanded CUG and CTG Repeats as a Therapeutic Approach for Myotonic Dystrophy Type 1 (DM1)
DM1 is an RNA gain‐of‐function disease caused by CTG repeat expansion, producing toxic r(CUG)exp RNA that sequesters MBNL1 and impairs splicing. This review covers the field of CUG and CTG ligands identified or rationally designed as DM1 drug candidates, highlighting their molecular design, RNA‐ or DNA‐binding modes, in vitro affinities and ...
Camille Richagneux, Anton Granzhan
wiley +1 more source
Colorimetric loop-mediated isothermal amplification for the rapid and visual detection of hepatitis B virus. [PDF]
Khan SJ +4 more
europepmc +1 more source
Microfluidic devices for Loop mediated isothermal amplification
A Tupik +4 more
openaire +1 more source
Phase Separation of Nucleic Acids: Mechanisms, Properties, and Applications
Recent discoveries have shown that single‐stranded long‐chain nucleic acids can undergo temperature‐induced phase separation, enabling formation of micrometer‐sized condensates. This Minireview discusses the current mechanistic understanding of this phenomenon, highlights strategies for controlling the physical and chemical properties of these ...
Weixiang Chen +2 more
wiley +1 more source
Development of a Field-Deployable Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of Erysiphe corylacearum in Hazelnut. [PDF]
Barone MM +7 more
europepmc +1 more source
The Development and Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection of Spirometra mansoni in Dogs. [PDF]
Tan X +7 more
europepmc +1 more source

