Diagnosis of α-thalassaemia by colorimetric gap loop mediated isothermal amplification
α-Thalassaemia is an inherited haemoglobin disorder that results from the defective synthesis of α-globin protein. Couples whom both carry the α-thalassaemia 1 gene are at risk of having a foetus with the most severe thalassaemia, Hb Bart’s hydrops ...
Worakawee Chumworathayee +4 more
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Loop-Mediated Isothermal Amplification: From Theory to Practice
Increasing the accuracy of pathogen identification and reducing the duration of analysis remain relevant for modern molecular diagnostics up to this day. In laboratory and clinical practice, detection of pathogens mostly relies on methods of nucleic acid amplification, among which the polymerase chain reaction (PCR) is considered the "gold standard ...
Shirshikov, F. V., Bespyatykh, J. A.
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Loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) are two rapid isothermal amplification methods for detecting three common fungal root pathogens of cool-season turfgrass: Gaeumannomyces avenae, Magnaporthiopsis
Brijesh B. Karakkat +5 more
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Strategies for the Voltammetric Detection of Loop-Mediated Isothermal Amplification
Loop-mediated isothermal amplification (LAMP) is rapidly developing into an important tool for the point-of-use detection of pathogens for both clinical and environmental samples, largely due to its sensitivity, rapidity, and adaptability to portable ...
Jesse M. Marangoni +2 more
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Capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients [PDF]
We demonstrate a multiplexed loop mediated isothermal amplification (LAMP) assay for infectious disease diagnostics, where the analytical process flow of target pathogens genomic DNA is performed manually by moving magnetic beads through a series of ...
Cooper, Jonathan M. +3 more
core +1 more source
Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae [PDF]
The rise in carbapenem-resistant Enterobacteriaceae (CRE) infections has created a global health emergency, underlining the critical need to develop faster diagnostics to treat swiftly and correctly.
Garner, Omai B. +5 more
core +1 more source
Loop‐mediated isothermal amplification of single pollen grains [PDF]
AbstractThe polymerase chain reaction (PCR) has been a reliable and fruitful method for many applications in ecology. Nevertheless, unavoidable technical and instrumental requirements of PCR have limited its widespread application in field situations. The recent development of isothermal DNA amplification methods provides an alternative to PCR, which ...
Ali, Bektaş, Ignacio, Chapela
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Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology
In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine,
Stefano Panno +7 more
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Probe-free label system for rapid detection of Cronobacter genus in powdered infant formula
Cronobacter species previously known as Enterobacter sakazakii poses high risks to neonates and infants. In this work a rapid detection method was developed which combined loop-mediated isothermal amplification with lateral flow assay for detection of ...
Shiqian Fu +6 more
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The isothermal amplification method, a molecular-based diagnostic technology, such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), is widely used as an alternative to the time-consuming and labor-intensive
Ye-Ji Moon, So-Young Lee, Se-Wook Oh
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