Results 241 to 250 of about 14,115 (284)

Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts. [PDF]

Proc Natl Acad Sci U S A
Kusuma SH, Kakizuka T, Hattori M, Nagai T.   +3 more
europepmc   +1 more source

A Highly Sensitive BRET-Based Reporter for Live-Cell Detection of HIV-1 Protease Activity and Inhibitor Screening. [PDF]

Viruses
Centazzo M, Djossou AV, Pavan S, Alvisi G.   +3 more
europepmc   +1 more source

Genome assembly of Genji firefly (Nipponoluciola cruciata) reveals novel luciferase-like luminescent proteins without peroxisome targeting signal. [PDF]

DNA Res
Fukuta K, Kato DI, Maeda J, Tsuruta A, Suzuki H, Nagano Y, Tsukamoto H, Niwa K, Terauchi M, Toyoda A, Fujiyama A, Noguchi H.   +11 more
europepmc   +1 more source

A Comprehensive Exploration of Bioluminescence Systems, Mechanisms, and Advanced Assays for Versatile Applications. [PDF]

Biochem Res Int
Dunuweera AN, Dunuweera SP, Ranganathan K.   +2 more
europepmc   +1 more source

A Structural Model of Truncated Gaussia princeps Luciferase Elucidating the Crucial Catalytic Function of No.76 Arginine towards Coelenterazine Oxidation. [PDF]

PLoS Comput Biol
Wu N, Xu ZC, Du KD, Huang S, Kobayashi N, Kuroda Y, Bai YH.   +6 more
europepmc   +1 more source

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Crystalline firefly luciferase

Biochimica et Biophysica Acta, 1956
Abstract 1. 1. The method for purification and crystallization of firefly luciferase is described. 2. 2. The crystalline enzyme is apparently homogenous as evidenced by ultracentrifugation a and electrophoretic determinations. The molecular weight is probably around 100,000 and the isoelectric point between pH 6.2 and 6.3. 3. 3.
A A, GREEN, W D, MCELROY
openaire   +2 more sources

Bioluminescence assays: multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay

Expert Opinion on Drug Discovery, 2010
By selecting the most appropriate bioluminescence assay, the researcher can study the underlying molecular mechanisms of a physiological system and the effects of a drug throughout the body.This review covers three luciferase assay systems: the multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. These assays are applied
Yoshihiro, Nakajima, Yoshihiro, Ohmiya
openaire   +2 more sources

Firefly luciferase inhibition

Journal of Photochemistry and Photobiology B: Biology, 2010
Firefly luciferase (Luc) is the most studied of the luciferase enzymes and the mechanism and kinetics of the reactions catalyzed by this enzyme have been relatively well characterized. Luc catalyzes the bioluminescent reaction involving firefly luciferin (D-LH(2)), adenosine triphosphate (ATP), magnesium ion and molecular oxygen with the formation of ...
João M. M. Leitão, Joaquim C. G. Esteves da Silva   +1 more
openaire   +3 more sources