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Lymphatic Endothelial Cells of the Lymph Node
Nature Reviews Immunology, 2020The influx and efflux of cells and antigens to and from the draining lymph nodes largely take place through the subcapsular, cortical and medullary sinus systems. Recent analyses in mice and humans have revealed unexpected diversity in the lymphatic endothelial cells, which form the distinct regions of the sinuses.
Marko Salmi, Sirpa Jalkanen
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Detection, Isolation and Culture of Lymphatic Endothelial Cells
, 2004 Lymphatic vessels are essential for the maintenance of normal tissue fluid balance and immune surveillance, but they also provide a pathway for metastasis in many types of cancers (reviewed in Alitalo and Carmeliet 2002). In spite of the importance of lymphatic vessels in medicine, the cell biology of this part of the vascular system has received ...
Mäkinen, T., Alitalo, K.
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Mechanotransduction in lymphatic endothelial cells.
Lymphology, 2007 Initial lymphatic vessel endothelial cells are connected to the surrounding elastic fibers by fibrillin anchoring filaments that have been hypothesized to favor interstitial fluid drainage in edema pulling apart interendothelial junctions. We hypothesized a biochemical mechanism involving mechanotransduction. This study was designed to verify whether a
ROSSI, A. +5 more
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Isolation, Identification, and Culture of Human Lymphatic Endothelial Cells
Methods in Molecular Biology, 2016A protocol describing the isolation of foreskin lymphatic endothelial cells (LECs) and lymphatic malformation lymphatic endothelial cells (LM LECs) is presented herein. To isolate LECs and LM LECs, tissues are mechanically disrupted to make a single-cell suspension, which is then enzymatically digested in dispase and collagenase type II.
Zerina Lokmic
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Murine Dermal Lymphatic Endothelial Cell Isolation
Journal of Visualized Experiments, 2023The lymphatic system participates in the regulation of immune surveillance, lipid absorption, and tissue fluid balance. The isolation of murine lymphatic endothelial cells is an important process for lymphatic research, as it allows the performance of in vitro and biochemical experiments on the isolated cells.
Racheal Grace, Akwii +7 more
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Lymphatic endothelial cell inclusion bodies
Journal of Ultrastructure Research, 1980Paracrystalline, compact tubular, and myeloid inclusion bodies were observed in endothelial cells from canine renal, mesenteric, and thoracic duct lymphatics. Paracrystalline inclusions were membrane bound and were composed of filaments organized into closely packed hexagons (18 nm across).
K H, Albertine, L M, Fox, C C, O'Morchoe
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Harvesting and cryopreservation of lymphatic endothelial cells for lymphatic tissue engineering
Cryobiology, 2010In order to provide a suitable source of cells for lymphatic tissue engineering, the present study was designed to investigate techniques for harvesting and cryopreservation of human dermal lymphatic endothelial cells (LECs) in vitro. The LECs were isolated from children's foreskins and then cultured in endothelial growth medium-2 MV (EGM-2-MV) with 5%
Zhaohua, Jiang +3 more
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Gene expression profile of lymphatic endothelial cells
Cell Biology International, 2011The lymphatic system was first described at around the same time as the blood circulation centuries ago, but the biological function elucidation of LECs (lymphatic endothelial cells) is far less than that of BVECs (blood vascular endothelial cells). Since the discovery of molecular markers for LECs and exploration of lymphatic role in tumour metastasis,
Peiliang, Wang, Yongbo, Cheng
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Establishment of Rat Lymphatic Endothelial Cell Line
Microcirculation, 2003The objective of the present study was to establish a rat lymphatic endothelial cell line and then to investigate the morphological and immunohistochemical properties of the cells.The lymphatic endothelial cells of rat thoracic ducts were isolated enzymatically by trypsin digestion and were cultured in endothelium growth medium (EGM)-2 in an atmosphere
Risuke, Mizuno +4 more
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