Results 141 to 150 of about 1,085 (178)
Some of the next articles are maybe not open access.
Lyoprotection of aviscumine with low molecular weight dextrans
International Journal of Pharmaceutics, 2003The aim of this research was to ascertain whether dextrans with low molecular weight will stabilize aviscumine. During freeze-drying increasing concentrations of dextran T1 (MW 1000) stabilized aviscumine. Eight percent of dextran resulted in a nearly 100% recovery of the activity and in addition a complete amorphous structure of the solid phase was ...
O, Gloger, K, Witthohn, B W, Müller
openaire +2 more sources
Food Chemistry, 2023
Nanoliposome is a promising delivery system, whereas its commercial application is limited by the structural instability, cargo leakage and particles aggregation during the processing such as freeze-drying. In this study, the effect of four oligosaccharides, fructo-oligosaccharides, lactose, inulin and sucrose (control), on the physicochemical ...
Hanyun Jiang +6 more
openaire +2 more sources
Nanoliposome is a promising delivery system, whereas its commercial application is limited by the structural instability, cargo leakage and particles aggregation during the processing such as freeze-drying. In this study, the effect of four oligosaccharides, fructo-oligosaccharides, lactose, inulin and sucrose (control), on the physicochemical ...
Hanyun Jiang +6 more
openaire +2 more sources
Maltodextrins as Lyoprotectants in the Lyophilization of a Model Protein, LDH
Pharmaceutical Research, 1996Maltodextrins, partially hydrolysed starches, were evaluated as potential lyoprotectants and the effect of combinations of maltodextrins and PEG 8000 on the protection of lactate dehydrogenase (LDH) was examined.LDH activity assays were performed immediately before freezing and after reconstruction.
S, Corveleyn, J P, Remon
openaire +2 more sources
Effect of γ-cyclodextrin as a lyoprotectant for freeze-dried actinidin
Die Pharmazie, 2015Actinidin (ATD) is a cysteine protease found in kiwifruit. It is used to tenderize meat and to enhance the digestion of proteins in the small intestine. However, ATD is unstable during freeze-drying, which alters its bioactivity. It is well known that sugars have the ability to protect proteins from the stress of freeze-drying.
Furuishi, T. +8 more
openaire +2 more sources
Dramatic enhancement of enzymatic activity in organic solvents by lyoprotectants
Biotechnology and Bioengineering, 1993AbstractWhen seven different hydrolytic enzymes (four proteases and three lipases) were lyophilized from aqueous solution containing a ligand, N‐Ac‐L‐Phe‐NH2, their catalytic activity in anhydrous solvents was far greater (one to two orders of magnitude) than that of the enzymes lyophilized without the ligand.
K, Debulis, A M, Klibanov
openaire +2 more sources
Sugar-mediated lyoprotection of purified human CYP3A4 and CYP2D6
Journal of Biotechnology, 2007P450 enzymes are of great interest for drug metabolism and as potential biocatalysts. Like most P450s, purified CYP3A4 is normally handled and stored in solution because lyophilization greatly reduces its activity. We show here that colyophilization of this enzyme with sucrose or trehalose, but not mannitol, crown ethers or cyclodextrins, allow ...
Amandine, Chefson +2 more
openaire +2 more sources
Short term stability of freeze-dried, lyoprotected liposomes
Journal of Controlled Release, 1999In the present study we examined the short term stability of liposomes in the freeze-dried state for different lipid compositions containing trehalose as a lyoprotectant. The retention of carboxyfluorescein and average vesicle size after rehydration were monitored as a function of the temperature to which the dry cakes were exposed for 0.5 h.
van Winden, E.C.A., Crommelin, D.J.A.
openaire +3 more sources
Food Research International, 2023
Bacteriophages as promising natural antibacterial additives are widely used in food processing and storage. Although freeze-drying is an economical and efficient way to preserve phages, so far there is limited data for phage freeze-drying and key factors that inactivate phages during freeze-drying and storage remain unknown.
openaire +2 more sources
Bacteriophages as promising natural antibacterial additives are widely used in food processing and storage. Although freeze-drying is an economical and efficient way to preserve phages, so far there is limited data for phage freeze-drying and key factors that inactivate phages during freeze-drying and storage remain unknown.
openaire +2 more sources
Lyoprotectants modify and stabilize self-assembly of polymersomes
Polymer, 2016Abstract Polymersome formation in water was confirmed with average polymersome diameters of 237.2 ± 66.5 nm over 150 min. Empty polymersomes created by dissolving poly(ethylene glycol)-b-poly(lactic acid) in water increased to 4.63 ± 0.01 times their size after lyophilization, showing lack of long-term stability.
Jessica M. Kelly +3 more
openaire +1 more source
Effect of lyoprotectants on ascorbate oxidase activity after freeze-drying and storage
Process Biochemistry, 1996Abstract The activity of ascorbate oxidase (AO) purified from green zucchini squash ( Cucurbita pepo medullosa var. Milano) decreased on freeze-drying. In rehydrated samples, enzyme activity was strictly correlated with the addition of lyoprotectants, the freeze temperature, the enzyme concentration and the storage time.
D'ANDREA, Gabriele +2 more
openaire +2 more sources