Characterisation of heat-induced protein aggregation in whey protein isolate and the influence of aggregation on the availability of amino groups as measured by the ortho-phthaldialdehyde (OPA) and trinitrobenzenesulfonic acid (TNBS) methods [PDF]
, 2017 Whey protein isolate (WPI) solutions, with different levels of aggregated protein, were prepared by heating (5% protein, pH 7, 90 °C for 30 min) WPI solutions with either 20 mM added NaCl (WPI + NaCl), 5 mM N-ethylmaleimide (WPI + NEM) or 20 mM added ...
Fargier-Lagrange, Maéva +3 more
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, 2023 Lysine acylations are reversible and ubiquitous post-translational modifications that play critical roles in regulating multiple cellular processes. In the current study, highly abundant and dynamic acetylation, besides succinylation, was uncovered in a soil bacterium, Streptomyces coelicolor.
openaire +1 more source
HAMAP in 2015: updates to the protein family classification and annotation system [PDF]
, 2017 HAMAP (High-quality Automated and Manual Annotation of Proteins—available at http://hamap.expasy.org/) is a system for the automatic classification and annotation of protein sequences. HAMAP provides annotation of the same quality and detail as UniProtKB/
Auchincloss, Andrea H. +12 more
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Signal Transduction and Targeted TherapyPosttranslational modification succinylation plays a pivotal role in tumorigenesis across malignancies, yet its mechanistic contributions to hepatocellular carcinoma (HCC) pathogenesis and therapeutic resistance remain poorly characterized. In this study,
Jun Wu +14 more
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Biochemical Analysis of Histone Succinylation
Biochemistry Research International, 2017 Posttranslational modification (PTM) of proteins is used to regulate protein activity and stability. Histone PTMs are regarded as some of the most important, as they can directly regulate gene expression through chromatin reorganization.
Atsushi Yokoyama +2 more
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Carnitine Palmitoyltransferase 1A Has a Lysine Succinyltransferase Activity
Cell Reports, 2018 Summary: Lysine succinylation was recently identified as a post-translational modification in cells. However, the molecular mechanism underlying lysine succinylation remains unclear. Here, we show that carnitine palmitoyltransferase 1A (CPT1A) has lysine
Kiran Kurmi +8 more
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A quantitative atlas of histone modification signatures from human cancer cells [PDF]
, 2013 BACKGROUND: An integral component of cancer biology is the understanding of molecular properties uniquely distinguishing one cancer type from another. One class of such properties is histone post-translational modifications (PTMs).
Blanco, MA +10 more
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Protein succinylation: regulating metabolism and beyond
Frontiers in NutritionModifications of protein post-translation are critical modulatory processes, which alters target protein biological activity，function and/or location, even involved in pathogenesis of some diseases. So far, there are at least 16 types of post-translation
Xiaoli Hou +7 more
doaj +1 more source
Maximal Oxidative Capacity during Exercise Is Associated with Skeletal Muscle Fuel Selection and Dynamic Changes in Mitochondrial Protein Acetylation [PDF]
, 1995 SummaryMaximal exercise-associated oxidative capacity is strongly correlated with health and longevity in humans. Rats selectively bred for high running capacity (HCR) have improved metabolic health and are longer-lived than their low-capacity ...
Overmyer, Katherine A. +10 more
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A Semi-Synthetic Method for Sythesis of SUMOylated Hypoxia Inducible Factor-1 alpha for Future Functional Analysis [PDF]
, 2011 : Native chemical ligation (NCL) is a method used to synthesize a covalent bond between two proteins. The method has been extensively used to react a C-terminal thioester on one protein with an N-terminal cysteine residue on another protein.
Heater, Bradley
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