Results 91 to 100 of about 108,965 (216)

Construction and analysis of a genetically tuneable lytic phage display system [PDF]

Applied Microbiology and Biotechnology, 2013
The Bacteriophage λ capsid protein gpD has been used extensively for fusion polypeptides that can be expressed from plasmids in Escherichia coli and remain soluble. In this study, a genetically controlled dual expression system for the display of enhanced green fluorescent protein (eGFP) was developed and characterized.
Nicastro, Jessica, Sheldon, Katlyn, El-zarkout, Farah A., Sokolenko, Stanislav, Aucoin, Marc, Slavcev, Roderick   +5 more
openaire   +3 more sources

Isolation and Characterization of a Helicobacter pylori Bacteriophage from a Sewage Sample at a Tertiary Hospital in Northern Iran [PDF]

Journal of Mazandaran University of Medical Sciences
Background and purpose: Helicobacter pylori is a Gram-negative, microaerophilic, and spiral-shaped bacterium recognized as a major causative agent of gastrointestinal diseases such as chronic gastritis, peptic ulcers, and gastric cancer.
Golnar Rahimzadeh, Mohammad Sadegh Rezai, Nasrin Samadi, Jamalifar, Mohammad Reza Fazeli   +4 more
doaj  

Polycationic Peptide Engineering of Phage Endolysins Expands Host Range and Enhances Antibacterial and Antibiofilm Activities Against Bacillus Species

Biotechnology and Bioengineering, Volume 123, Issue 10, Page 2788-2801, October 2026.
ABSTRACT The exponential rise in antimicrobial resistance has highlighted the urgent need for the development of alternative antibacterial strategies, including phage‐derived and engineered protein agents such as endolysins and artilysins. In this study, we report the rational design, expression, and functional characterization of novel artilysins ...
Farhat Ansari, Tarushi, Vandan Nagar
wiley   +1 more source

Genomic and Phenotypic Characterization of Two Novel Enterobacter Phages With EDTA‐Enhanced Antibiofilm Activity

MicrobiologyOpen, Volume 15, Issue 5, October 2026.
Two novel lytic Enterobacter phages, HHH and RZH, displayed targeted antibiofilm activity against MDR Enterobacter cloacae complex biofilms when combined with EDTA. Activity was strongest within a narrow low‐millimolar EDTA window, reducing biofilm biomass and viable burden, while higher EDTA concentrations shifted the interaction towards antagonism ...
Kashif Haq, Zac Newland‐Smith, Martin Figgit, David Lee, Kwabena Duedu   +4 more
wiley   +1 more source

Estimation of the In-Vitro Synergistic Effect of Vancomycin with Bacteriophage and its Endolysin on Iraqi Local Isolates of Enterococcus faecalis

مجلة بغداد للعلوم
Enterococcus faecalis is known to cause many serious infections with an upsurge in the development of antibiotic resistant strains, especially vancomycin.
Rand R. Hafidh, Zainab Oday Hamed, Abdullah A. Awni, Manal N. Jassas   +3 more
doaj   +1 more source

Quantifying Viral Lysis in Microalgae Using Cell‐Free rRNA

MicrobiologyOpen, Volume 15, Issue 5, October 2026.
Cell‐free ribosomal RNA (rRNA) dissolved in seawater provides a quantitative proxy for viral lysis in microeukaryotic phytoplankton. Incubation experiments with two distinct host–virus systems revealed marked increases in cell‐free rRNA production per cell, a proxy for lytic mortality, reaching up to 46‐ and 302‐fold relative to noninfected controls ...
Saki Kikuya, Yuji Tomaru, Keizo Nagasaki, Daichi Morimoto, Yuki Yamagishi, Hiroyuki Ogata, Hisashi Endo   +6 more
wiley   +1 more source

Self-assembly of Escherichia coli phage shock protein A

, 2014
The Phage shock protein (Psp) response is an extracytoplasmic stress response. The central component of this system is PspA, a protein that mediates the physiological response to membrane stress.
Male, Abigail, Tavassoli, Ali
core   +1 more source

Obtaining a lytic bacteriophage suitable for use in phage therapy against Clostridioides difficile infection [PDF]

, 2023
Clostridioides difficile is the leading cause of hospital-acquired and antibiotic-associated diarrhoea in the developed world. More effective countermeasures are required.
Kerr, Sarah
core  

Antibacterial Activity of a Lytic Enzyme Encoded by Pseudomonas aeruginosa Double Stranded RNA Bacteriophage phiYY

Frontiers in Microbiology, 2018
Multidrug-resistant Pseudomonas aeruginosa is one of the most life-threatening pathogens for global health. In this regard, phage encoded lytic proteins, including endolysins and virion-associated peptidoglycan hydrolases (VAPGH), have been proposed as ...
Yuhui Yang, Shuai Le, Wei Shen, Qian Chen, Youying Huang, Shuguang Lu, Yinling Tan, Ming Li, Fuquan Hu, Yang Li   +9 more
doaj   +1 more source

An Integrated System for Geobacillus Protoplast Formation, Transfection, Recombinant Genomes Rebooting, Efficient Propagation, and Genomic DNA Isolation of the Thermophilic Bacteriophage TP‐84

MicrobiologyOpen, Volume 15, Issue 5, October 2026.
The study presents an optimized, reproducible protoplast transfection method for Geobacillus stearothermophilus. The method enables efficient uptake of native and fully synthetic TP‐84 bacteriophage genomes, their “rebooting” in vivo and production of infectious phage particles.
Ireneusz Sobolewski, Katarzyna Adamowicz, Jan Chodorski, Piotr M. Skowron, Agnieszka Zylicz‐Stachula   +4 more
wiley   +1 more source