Results 121 to 130 of about 4,722 (203)

Influence of microbiota on the growth and gene expression of Clostridioides difficile in an in vitro coculture model

MicrobiologyOpen, Volume 13, Issue 5, October 2024.
The presence of microbiota did not affect the growth of Clostridioides difficile in this study. However, it did influence the expression of C. difficile genes related to sporulation, germination, and virulence, which are crucial for the transmission of the pathogen. In the presence of microbiota, C.
Elisa Martinez, Noémie Berg, Cristina Rodriguez, Georges Daube, Bernard Taminiau   +4 more
wiley   +1 more source

Crystal Structure of MltA from Escherichia coli Reveals a Unique Lytic Transglycosylase Fold [PDF]

Journal of Molecular Biology, 2005
Lytic transglycosylases are bacterial enzymes involved in the maintenance and growth of the bacterial cell-wall peptidoglycan. They cleave the beta-(1,4)-glycosidic bonds in peptidoglycan forming non-reducing 1,6-anhydromuropeptides. The crystal structure of the lytic transglycosylase MltA from Escherichia coli without a membrane anchor was solved at 2.
van Straaten KE, Dijkstra BW, Vollmer W, Thunnissen AMWH   +3 more
openaire   +3 more sources

Diversity and Distribution of a Novel Genus of Hyperthermophilic Aquificae Viruses Encoding a Proof-Reading Family-A DNA Polymerase

Frontiers in Microbiology, 2020
Despite the high abundance of Aquificae in many geothermal systems, these bacteria are difficult to culture and no viruses infecting members of this phylum have been isolated. Here, we describe the complete, circular dsDNA Uncultivated Virus Genome (UViG)
Marike Palmer, Brian P. Hedlund, Brian P. Hedlund, Simon Roux, Philippos K. Tsourkas, Philippos K. Tsourkas, Ryan K. Doss, Casey Stamereilers, Astha Mehta, Jeremy A. Dodsworth, Michael Lodes, Scott Monsma, Tijana Glavina del Rio, Thomas W. Schoenfeld, Emiley A. Eloe-Fadrosh, David A. Mead   +15 more
doaj   +1 more source

A metagenomic survey of the fecal microbiome of the African savanna elephant (Loxodonta africana)

Animal Genetics, Volume 55, Issue 4, Page 621-643, August 2024.
Abstract The African savanna elephant (Loxodonta africana) is the largest terrestrial animal on Earth and is found primarily in Southern and Eastern Africa. It is a hindgut, colonic fermenter and subsists on a diet of raw plant materials found in its grazing area.
Louis Lategan du Preez, Elzette van der Walt, Angel Valverde, Christopher Rothmann, Frederick Wilhelm Cornelius Neser, Errol Duncan Cason   +5 more
wiley   +1 more source

DUF3380 domain from a Salmonella phage endolysin shows potent N -acetylmuramidase activity [PDF]

, 2016
Bacteriophage-encoded endolysins are highly diverse enzymes that cleave the bacterial peptidoglycan layer. Current research focuses on their potential applications in medicine, in food conservation, and as biotechnological tools.
Hans Gerstmans, Lorena Rodríguez-Rubio, M. J. Pettinari, Rob Lavigne, Simon Thorpe, Stéphane Mesnage, Yves Briers   +6 more
core   +1 more source

Cleavage of an engulfment peptidoglycan hydrolase by a sporulation signature protease in Clostridioides difficile

Molecular Microbiology, Volume 122, Issue 2, Page 213-229, August 2024.
Model for the cleavage of SpoIIP by SpoIVB2 during sporulation in Clostridioides difficile. Soon after engulfment completion, SpoIVB2 cleaves SpoIIP, part of the engulfasome complex, to generate the SpoIIPc form. The function of SpoIVB2 is required for synthesis of the cortex layer which takes place across the outer forespore membrane.
Diogo Martins, Hailee N. Nerber, Charlotte G. Roughton, Amaury Fasquelle, Anna Barwinska‐Sendra, Daniela Vollmer, Joe Gray, Waldemar Vollmer, Joseph A. Sorg, Paula S. Salgado, Adriano O. Henriques, Mónica Serrano   +11 more
wiley   +1 more source

Characterization and Genome Sequence of Marine Alteromonas gracilis Phage PB15 Isolated from the Yellow Sea, China [PDF]

, 2017
A novel marine Alteromonas gracilis siphovirus, phage PB15, was isolated from the surface water of the Yellow Sea in August 2015. It has a head diameter of 58 ± 5 nm head and a contractile tail approximately 105 ± 10 nm in length, and overall the ...
Gao, Yu, Gong, Zheng, Jiang, Yong, Li, Yutong, Lin, Tongtong, Liu, Qian, Liu, Zhaoyang, Malin, Gill, Meng, Xue, Shao, Hongbing, Wang, Min, Zhao, Guihua   +11 more
core   +1 more source

Determinants in the phage life cycle: The dynamic nature of ssDNA phage FLiP and host interactions under varying environmental conditions and growth phases

Environmental Microbiology, Volume 26, Issue 7, July 2024.
The interactions between the lipid‐containing ssDNA phage FLiP and its three Flavobacterium hosts are influenced by factors such as the host strain and culture conditions, including temperature and bacterial growth phase. Abstract The influence of environmental factors on the interactions between phages and bacteria, particularly single‐stranded DNA ...
Kati Mäkelä, Elina Laanto, Lotta‐Riina Sundberg   +2 more
wiley   +1 more source

The DigH glycosyl hydrolase is conditionally required for daughter cell separation in Escherichia coli

Journal of Bacteriology
The peptidoglycan (PG) cell wall is a mesh-like layer that shapes bacteria and protects against osmotically induced lysis. PG is composed of glycan strands and peptide chains that link together to form a continuous layer that surrounds the cell.
Joseph C. Bryant, Emily J. Robbs, Alongkorn Kurilung, Brittney A. Dinkel, Intawat Nookaew, Matthew A. Jorgenson   +5 more
doaj   +1 more source

Crystal structure of the PepSY-containing domain of the YpeB protein involved in germination of bacillus spores. [PDF]

, 2015
The crystal structure of the C-terminal domain of the Bacillus megaterium YpeB protein has been solved by X-ray crystallography to 1.80-Å resolution. The full-length protein is essential in stabilising the SleB cortex lytic enzyme in Bacillus spores, and
Chirgadze, Dimitri Y, Christie, Graham, Üstok, Fatma Işık   +2 more
core   +1 more source