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Reading m6A in the Transcriptome: m6A-Binding Proteins [PDF]

Trends in Cell Biology, 2018
N6-Methyladenosine (m6A) is the most prevalent post-transcriptional modification of eukaryotic mRNA and long noncoding RNA. m6A mediates its effects primarily by recruiting proteins, including the multiprotein eukaryotic initiation factor 3 complex and a set of proteins that contain the YTH domain.
Deepak P Patil, Samie R Jaffrey
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Mapping single-nucleotide m6A by m6A-REF-seq

Methods, 2022
The past few years have witnessed rapid progress in the field of RNA modifications. As the most prevailing modification on eukaryotic mRNA, m6A is characterized to play a vital role in various cellular activities. However, limitations of the detection method impede functional studies of m6A. Here we introduce m6A-REF-seq, a powerful and straightforward
Hong-Xuan, Chen, Zhang, Zhang, Dong-Zhao, Ma, Li-Qian, Chen, Guan-Zheng, Luo   +4 more
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m6A-SAC-seq for quantitative whole transcriptome m6A profiling

Nature Protocols, 2022
N6-methyladenosine (m6A) is the most abundant mRNA modification in mammalian cells, regulating many physiological processes. Here we describe a method for base-resolution, quantitative m6A sequencing in the whole transcriptome. The enzyme and small-molecule cofactor used in this protocol are prepared by recombinant protein expression and organic ...
Ruiqi Ge, Chang Ye, Yong Peng, Qing Dai, Yutao Zhao, Shun Liu, Pingluan Wang, Lulu Hu, Chuan He   +8 more
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m6A readers, writers, erasers, and the m6A epitranscriptome in breast cancer

Journal of Molecular Endocrinology, 2023
Epitranscriptomic modification of RNA regulates human development, health, and disease. The true diversity of the transcriptome in breast cancer including chemical modification of transcribed RNA (epitranscriptomics) is not well understood due to limitations of technology and bioinformatic analysis.
Belinda J Petri, Carolyn M Klinge
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m6A-Maize: Weakly supervised prediction of m6A-carrying transcripts and m6A-affecting mutations in maize (Zea mays)

Methods, 2022
With the rapid development of high-throughput sequencing techniques nowadays, extensive attention has been paid to epitranscriptomics, which covers more than 150 distinct chemical modifications to date. Among that, N6-methyladenosine (m6A) modification has the most abundant existence, and it is also significantly related to varieties of biological ...
Zhanmin, Liang, Lei, Zhang, Haoting, Chen, Daiyun, Huang, Bowen, Song   +4 more
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The epitranscriptome beyond m6A

Nature Reviews Genetics, 2020
Following its transcription, RNA can be modified by >170 chemically distinct types of modifications - the epitranscriptome. In recent years, there have been substantial efforts to uncover and characterize the modifications present on mRNA, motivated by the potential of such modifications to regulate mRNA fate and by discoveries and advances in our ...
David Wiener, Schraga Schwartz
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Resolving m6A epitranscriptome with stoichiometry

Trends in Genetics, 2022
A recent study by Hu et al. describes N6-methyladenosine (m6A)-selective allyl chemical labeling and sequencing (m6A-SAC-seq), which allows for quantitative, stoichiometric, and positional analyses of m6A at single-nucleotide resolution across the whole transcriptome level.
Ki-Jun Yoon, Yoon Ki Kim
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Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq

Nature, 2012
An extensive repertoire of modifications is known to underlie the versatile coding, structural and catalytic functions of RNA, but it remains largely uncharted territory. Although biochemical studies indicate that N(6)-methyladenosine (m(6)A) is the most prevalent internal modification in messenger RNA, an in-depth study of its distribution and ...
Dan, Dominissini, Sharon, Moshitch-Moshkovitz, Schraga, Schwartz, Mali, Salmon-Divon, Lior, Ungar, Sivan, Osenberg, Karen, Cesarkas, Jasmine, Jacob-Hirsch, Ninette, Amariglio, Martin, Kupiec, Rotem, Sorek, Gideon, Rechavi   +11 more
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An update on principles of m6A targeting

Trends in Plant Science, 2022
N6-methyladenosine (m6A) is of fundamental importance in gene regulation. The function of m6A is achieved through proteins that recognize m6A, known as EVOLUTIONARILY CONSERVED C-TERMINAL REGIONS (ECTs) in arabidopsis (Arabidopsis thaliana). mRNA targets of ECTs and their interaction with m6A-containing motifs remain to be revealed.
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m6A Modification and Implications for microRNAs

MicroRNA, 2017
RNA is chemically modified with over 100 distinct reactions. Among these reactions, methylation is probably the most extensively studied modification on the RNA molecule. Studies suggest methylation of Adenine residues (m6A) to be widespread in the transcriptome with potentially important roles in biological processes.Here, we review recent literature ...
Ayse Elif, Erson-Bensan, Oguzhan, Begik
openaire   +2 more sources