Localization of protein aggregation in Escherichia coli is governed by diffusion and nucleoid macromolecular crowding effect. [PDF]
PLoS Computational Biology, 2013 Aggregates of misfolded proteins are a hallmark of many age-related diseases. Recently, they have been linked to aging of Escherichia coli (E. coli) where protein aggregates accumulate at the old pole region of the aging bacterium.
Anne-Sophie Coquel +8 more
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Macromolecular crowding and decellularization method increase the growth factor binding potential of cell-secreted extracellular matrices [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Recombinant growth factors are used in tissue engineering to stimulate cell proliferation, migration, and differentiation. Conventional methods of growth factor delivery for therapeutic applications employ large amounts of these bioactive cues. Effective,
Shierly W. Fok +6 more
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Cell wall damage increases macromolecular crowding effects in the Escherichia coli cytoplasm [PDF]
iScience, 2023 Summary: The intracellular milieu is crowded with biomacromolecules. Macromolecular crowding changes the interactions, diffusion, and conformations of biomacromolecules.
Theodoros Pittas +2 more
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Comparative analysis of different macromolecular crowding agents in human tenocyte cultures [PDF]
Biomaterials and BiosystemsTendon engineered substitutes remain clinically unavailable, largely due to the need for prolonged culture periods to allow for sufficient extracellular matrix deposition.
Andrea Rossoni +3 more
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A Comparative Study on Cyanine Dyestuffs as Sensor Candidates for Macromolecular Crowding In Vitro and In Vivo [PDF]
Biosensors, 2023 Pseudo isocyanine chloride (PIC) has been identified in a preceding work as a sensor suited to probe macromolecular crowding both in test tubes with solutions of synthetic crowding agents and in HeLa cells as a representative of living systems.
Leon Koch +3 more
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Gums as Macromolecular Crowding Agents in Human Skin Fibroblast Cultures [PDF]
LifeEven though tissue-engineered medicines are under intense academic, clinical, and commercial investigation, only a handful of products have been commercialised, primarily due to the costs associated with their prolonged manufacturing.
Salome Guillaumin +2 more
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Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture [PDF]
Cells, 2022 The absence of a native extracellular matrix and the use of xenogeneic sera are often associated with rapid tenocyte function losses during in vitro culture.
Andrea Rampin +6 more
doaj +2 more sources
Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis [PDF]
Materials Today BioAlthough human tenocytes and dermal fibroblasts have shown promise in tendon engineering, no tissue engineered medicine has been developed due to the prolonged ex vivo time required to develop an implantable device.
Adrian Djalali-Cuevas +11 more
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Macromolecular crowding explains overflow metabolism in cells [PDF]
Scientific Reports, 2016 Overflow metabolism is a metabolic phenotype of cells characterized by mixed oxidative phosphorylation (OxPhos) and fermentative glycolysis in the presence of oxygen.
Oltvai, Zoltán N., Vazquez, Alexei
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Macromolecular crowding limits growth under pressure. [PDF]
Nat Phys, 2022 Cells that grow in confined spaces eventually build up mechanical compressive stress. This growth-induced pressure (GIP) decreases cell growth. GIP is important in a multitude of contexts from cancer[1–3], to microbial infections[4], to biofouling, yet our understanding of its origin and molecular consequences remains limited.
Alric B +4 more
europepmc +6 more sources