Results 41 to 50 of about 103,280 (234)

Macromolecular Crowding Modulates Actomyosin Kinetics [PDF]

Biophysical Journal, 2016
Actomyosin kinetics is usually studied in dilute solutions, which do not reflect conditions in the cytoplasm. In cells, myosin and actin work in a dense macromolecular environment. High concentrations of macromolecules dramatically reduce the amount of free space available for all solutes, which results in an effective increase of the solutes' chemical
Jinghua, Ge, Sherry D, Bouriyaphone, Tamara A, Serebrennikova, Andrei V, Astashkin, Yuri E, Nesmelov   +4 more
openaire   +2 more sources

All atom insights into the impact of crowded environments on protein stability by NMR spectroscopy

Nature Communications, 2020
The precise effects of crowding on protein folding have been difficult to establish. Here the authors apply multidimensional high-resolution NMR spectroscopy to provide insight on the local impact of macromolecular crowding on the thermodynamic stability
Birgit Köhn, Michael Kovermann
doaj   +1 more source

How Molecular Crowding Differs from Macromolecular Crowding: A Femtosecond Mid-Infrared Pump–Probe Study

, 2018
Crowding is an inherent property of living systems in which biochemical processes occur in highly concentrated solutions of various finite-sized species of both low (molecular crowding) and high (macromolecular crowding) molecular weights.
Minhaeng Cho, Pramod Kumar Verma, Achintya Kundu   +2 more
core   +2 more sources

The effect of macromolecular crowding on the electrostatic component of barnase-barstar binding: a computational, implicit solvent-based study. [PDF]

PLoS ONE, 2014
Macromolecular crowding within the cell can impact both protein folding and binding. Earlier models of cellular crowding focused on the excluded volume, entropic effect of crowding agents, which generally favors compact protein states.
Helena W Qi, Priyanka Nakka, Connie Chen, Mala L Radhakrishnan   +3 more
doaj   +1 more source

Molecular Basis for Actin Polymerization Kinetics Modulated by Solution Crowding

Biomolecules, 2023
Actin polymerization drives cell movement and provides cells with structural integrity. Intracellular environments contain high concentrations of solutes, including organic compounds, macromolecules, and proteins.
Bryan Demosthene, Myeongsang Lee, Ryan R. Marracino, James B. Heidings, Ellen Hyeran Kang   +4 more
doaj   +1 more source

RNA: The Unsuspected Conductor in the Orchestra of Macromolecular Crowding. [PDF]

Chem Rev
This comprehensive Review delves into the chemical principles governing RNA-mediated crowding events, commonly referred to as granules or biological condensates. We explore the pivotal role played by RNA sequence, structure, and chemical modifications in these processes, uncovering their correlation with crowding phenomena under physiological ...
Zacco E, Broglia L, Kurihara M, Monti M, Gustincich S, Pastore A, Plath K, Nagakawa S, Cerase A, Sanchez de Groot N, Tartaglia GG.   +10 more
europepmc   +7 more sources

Effects of Macromolecular Crowding on the Collapse of Biopolymers

Physical Review Letters, 2015
Experiments show that macromolecular crowding modestly reduces the size of intrinsically disordered proteins (IDPs) even at volume fraction ($ϕ$) similar to that in the cytosol whereas DNA undergoes a coil-to-globule transition at very small $ϕ$. We show using a combination of scaling arguments and simulations that the polymer size $\overline{R}_g(ϕ ...
Kang, Hongsuk, Pincus, Philip A., Hyeon, Changbong, Thirumalai, D.   +3 more
openaire   +4 more sources

Impact of macromolecular crowding on DNA replication [PDF]

Nature Communications, 2013
Enzymatic activities in vivo occur in a crowded environment composed of many macromolecules. This environment influences DNA replication by increasing the concentration of the constituents, desolvation, decreasing the degrees of freedom for diffusion and hopping of proteins onto DNA, and enhancing binding equilibria and catalysis.
Akabayov, Barak, Akabayov, Sabine R., Wagner, Gerhard, Richardson, Charles, Lee, Seung–Joo   +4 more
openaire   +4 more sources

Macromolecular crowding and its potential impact on nuclear function [PDF]

, 2008
It is well established, that biochemical reactions are dependent on pH, ionic strength, temperature and the concentration of reactants. However, the steric repulsion among bulky components of biological systems also affect biochemical behavior: The ...
Nessling, Michelle, Lichter, Peter, Richter, Karsten   +2 more
core   +1 more source

Transforming eukaryotic cell culture with macromolecular crowding

, 2021
In multicellular organisms, the intracellular and extracellular spaces are considerably packed with a diverse range of macromolecular species. Yet, standard eukaryotic cell culture is performed in dilute, and deprived of macromolecules culture media ...
Raghunath, Michael, Zeugolis, Dimitrios I.   +1 more
core   +1 more source