Results 31 to 40 of about 6,829 (247)

Macromolecular crowding effects on the kinetics of opposing reactions catalyzed by alcohol dehydrogenase

Biochemistry and Biophysics Reports, 2021
In order to better understand how the complex, densely packed, heterogeneous milieu of a cell influences enzyme kinetics, we exposed opposing reactions catalyzed by yeast alcohol dehydrogenase (YADH) to both synthetic and protein crowders ranging from 10
Xander E. Wilcox, Charmaine B. Chung, Kristin M. Slade   +2 more
doaj   +1 more source

Adapting the Scar-in-a-Jar to Skin Fibrosis and Screening Traditional and Contemporary Anti-Fibrotic Therapies

Frontiers in Bioengineering and Biotechnology, 2021
Skin fibrosis still constitutes an unmet clinical need. Although pharmacological strategies are at the forefront of scientific and technological research and innovation, their clinical translation is hindered by the poor predictive capacity of the ...
João Q. Coentro, Ulrike May, Stuart Prince, John Zwaagstra, Olli Ritvos, Tero A.H. Järvinen, Tero A.H. Järvinen, Dimitrios I. Zeugolis, Dimitrios I. Zeugolis   +8 more
doaj   +1 more source

Impact of macromolecular crowding on DNA replication [PDF]

Nature Communications, 2013
Enzymatic activities in vivo occur in a crowded environment composed of many macromolecules. This environment influences DNA replication by increasing the concentration of the constituents, desolvation, decreasing the degrees of freedom for diffusion and hopping of proteins onto DNA, and enhancing binding equilibria and catalysis.
Akabayov, Barak, Akabayov, Sabine R., Wagner, Gerhard, Richardson, Charles, Lee, Seung–Joo   +4 more
openaire   +4 more sources

Macromolecular diffusion in crowded solutions

Biophysical Journal, 1993
The effects of crowding on the self or tracer diffusion of macromolecules in concentrated solutions is an important but difficult problem, for which, so far, there has been no rigorous treatment. Muramatsu and Minton suggested a simple model to calculate the diffusion coefficient of a hard sphere among other hard spheres.
Han, J., Herzfeld, J.
openaire   +2 more sources

Macromolecular Crowding Modulates Actomyosin Kinetics [PDF]

Biophysical Journal, 2016
Actomyosin kinetics is usually studied in dilute solutions, which do not reflect conditions in the cytoplasm. In cells, myosin and actin work in a dense macromolecular environment. High concentrations of macromolecules dramatically reduce the amount of free space available for all solutes, which results in an effective increase of the solutes' chemical
Jinghua, Ge, Sherry D, Bouriyaphone, Tamara A, Serebrennikova, Andrei V, Astashkin, Yuri E, Nesmelov   +4 more
openaire   +2 more sources

Conformation-changing enzymes and macromolecular crowding

Physical Chemistry Chemical Physics, 2021
We study how crowding affects the activity and catalysis-enhanced diffusion of enzymes and passive tracers by employing a fluctuating-dumbbell model of conformation-changing enzymes.
Tomasz Skóra, Mihail N. Popescu, Svyatoslav Kondrat   +2 more
openaire   +4 more sources

A combined physicochemical approach towards human tenocyte phenotype maintenance

Materials Today Bio, 2021
During in vitro culture, bereft of their optimal tissue context, tenocytes lose their phenotype and function. Considering that tenocytes in their native tissue milieu are exposed simultaneously to manifold signals, combination approaches (e.g.
C.N.M. Ryan, E. Pugliese, N. Shologu, D. Gaspar, P. Rooney, Md N. Islam, A. O'Riordan, M.J. Biggs, M.D. Griffin, D.I. Zeugolis   +9 more
doaj   +1 more source

All atom insights into the impact of crowded environments on protein stability by NMR spectroscopy

Nature Communications, 2020
The precise effects of crowding on protein folding have been difficult to establish. Here the authors apply multidimensional high-resolution NMR spectroscopy to provide insight on the local impact of macromolecular crowding on the thermodynamic stability
Birgit Köhn, Michael Kovermann
doaj   +1 more source

RNA: The Unsuspected Conductor in the Orchestra of Macromolecular Crowding. [PDF]

Chem Rev
This comprehensive Review delves into the chemical principles governing RNA-mediated crowding events, commonly referred to as granules or biological condensates. We explore the pivotal role played by RNA sequence, structure, and chemical modifications in these processes, uncovering their correlation with crowding phenomena under physiological ...
Zacco E, Broglia L, Kurihara M, Monti M, Gustincich S, Pastore A, Plath K, Nagakawa S, Cerase A, Sanchez de Groot N, Tartaglia GG.   +10 more
europepmc   +7 more sources

The effect of macromolecular crowding on the electrostatic component of barnase-barstar binding: a computational, implicit solvent-based study. [PDF]

PLoS ONE, 2014
Macromolecular crowding within the cell can impact both protein folding and binding. Earlier models of cellular crowding focused on the excluded volume, entropic effect of crowding agents, which generally favors compact protein states.
Helena W Qi, Priyanka Nakka, Connie Chen, Mala L Radhakrishnan   +3 more
doaj   +1 more source