Results 31 to 40 of about 103,280 (234)
Conformation-changing enzymes and macromolecular crowding
Physical Chemistry Chemical Physics, 2021 We study how crowding affects the activity and catalysis-enhanced diffusion of enzymes and passive tracers by employing a fluctuating-dumbbell model of conformation-changing enzymes.
Tomasz Skóra +2 more
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eLife, 2020 Cellular aging is a multifactorial process that is characterized by a decline in homeostatic capacity, best described at the molecular level. Physicochemical properties such as pH and macromolecular crowding are essential to all molecular processes in ...
Sara N Mouton +9 more
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Biochemistry and Biophysics Reports, 2021 In order to better understand how the complex, densely packed, heterogeneous milieu of a cell influences enzyme kinetics, we exposed opposing reactions catalyzed by yeast alcohol dehydrogenase (YADH) to both synthetic and protein crowders ranging from 10
Xander E. Wilcox +2 more
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Frontiers in Bioengineering and Biotechnology, 2021 Skin fibrosis still constitutes an unmet clinical need. Although pharmacological strategies are at the forefront of scientific and technological research and innovation, their clinical translation is hindered by the poor predictive capacity of the ...
João Q. Coentro +8 more
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Macromolecular crowding and membrane binding proteins:the case of phospholipase A1 [PDF]
, 2018 Cells contain high levels of macromolecular crowding; understanding how macromolecular crowding impacts the behaviour of biological systems can give new insights into biological phenomena and disease pathologies.
Mayoral-Delgado, Isabel +3 more
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Macromolecular diffusion in crowded solutions
Biophysical Journal, 1993 The effects of crowding on the self or tracer diffusion of macromolecules in concentrated solutions is an important but difficult problem, for which, so far, there has been no rigorous treatment. Muramatsu and Minton suggested a simple model to calculate the diffusion coefficient of a hard sphere among other hard spheres.
Han, J., Herzfeld, J.
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Macromolecular Crowding In Vitro , In Vivo , and In Between [PDF]
Trends in Biochemical Sciences, 2016 Biochemical processes take place in heterogeneous and highly volume-occupied or crowded environments that can considerably influence the reactivity and distribution of participating macromolecules. We summarize here the thermodynamic consequences of excluded-volume and long-range nonspecific intermolecular interactions for macromolecular reactions in ...
Germán, Rivas, Allen P, Minton
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A combined physicochemical approach towards human tenocyte phenotype maintenance
Materials Today Bio, 2021 During in vitro culture, bereft of their optimal tissue context, tenocytes lose their phenotype and function. Considering that tenocytes in their native tissue milieu are exposed simultaneously to manifold signals, combination approaches (e.g.
C.N.M. Ryan +9 more
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An upper limit for macromolecular crowding effects [PDF]
BMC Biophysics, 2011 Abstract Background Solutions containing high macromolecule concentrations are predicted to affect a number of protein properties compared to those properties in dilute solution. In cells, these macromolecular crowders have a large range of sizes and can occupy 30% or more of the available volume.
Miklos, Andrew C. +4 more
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Seaweed polysaccharides as macromolecular crowding agents
International Journal of Biological Macromolecules, 2020 Development of mesenchymal stem cell-based tissue engineered implantable devices requires prolonged in vitro culture for the development of a three-dimensional implantable device, which leads to phenotypic drift, thus hindering the clinical translation and commercialisation of such approaches.
De Pieri, Andrea +3 more
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