Results 21 to 30 of about 228,358 (272)
Resolving the enthalpy of protein stabilization by macromolecular crowding. [PDF]
Protein Sci, 2023 Proteins in the cellular milieu reside in environments crowded by macromolecules and other solutes. Although crowding can significantly impact the protein folded state stability, most experiments are conducted in dilute buffered solutions. To resolve the
Stewart CJ +4 more
europepmc +2 more sources
Direct activation of HSF1 by macromolecular crowding and misfolded proteins. [PDF]
PLoS OneStress responses play a vital role in cellular survival against environmental challenges, often exploited by cancer cells to proliferate, counteract genomic instability, and resist therapeutic stress.
Simoncik O +9 more
europepmc +2 more sources
Macromolecular crowding regulates matrix composition and gene expression in human gingival fibroblast cultures. [PDF]
Sci Rep, 2023 Standard cell cultures are performed in aqueous media with a low macromolecule concentration compared to tissue microenvironment. In macromolecular crowding (MMC) experiments, synthetic polymeric crowders are added into cell culture media to better mimic
Ramalingam R +3 more
europepmc +2 more sources
Macromolecular Crowding Is More than Hard-Core Repulsions
Annual Review of Biophysics, 2022 Cells are crowded, but proteins are almost always studied in dilute aqueous buffer. We review the experimental evidence that crowding affects the equilibrium thermodynamics of protein stability and protein association and discuss the theories employed to
Gary Pielak +2 more
exaly +2 more sources
Stabilization and improved functionality of three-dimensional perfusable microvascular networks in microfluidic devices under macromolecular crowding. [PDF]
Biomater Res, 2023 Background There is great interest to engineer in vitro models that allow the study of complex biological processes of the microvasculature with high spatiotemporal resolution. Microfluidic systems are currently used to engineer microvasculature in vitro
Wan HY +11 more
europepmc +2 more sources
Macromolecular crowding: Sensing without a sensor.
Current Opinion in Cell Biology, 2023 All living cells are crowded with macromolecules. Crowding can directly modulate biochemical reactions to various degrees depending on the sizes, shapes, and binding affinities of the reactants.
L. J. Holt, M. Delarue
semanticscholar +5 more sources
The Differential Response of Proteins to Macromolecular Crowding. [PDF]
PLoS Computational Biology, 2016 The habitat in which proteins exert their function contains up to 400 g/L of macromolecules, most of which are proteins. The repercussions of this dense environment on protein behavior are often overlooked or addressed using synthetic agents such as poly(
Michela Candotti, Modesto Orozco
doaj +5 more sources
Biochemistry and Biophysics Reports, 2021 In order to better understand how the complex, densely packed, heterogeneous milieu of a cell influences enzyme kinetics, we exposed opposing reactions catalyzed by yeast alcohol dehydrogenase (YADH) to both synthetic and protein crowders ranging from 10
Xander E. Wilcox +2 more
doaj +2 more sources
Macromolecular Crowding and Protein Aggregation: Friend, Foe or Contextual Force?
Progress in Biophysics and Molecular BiologyProtein aggregation plays a dual role in cellular biology, enabling essential functions such as intracellular organization, signaling, and storage, while also contributing to pathological states associated with misfolding and toxicity.
Isabella V. Gimón +2 more
semanticscholar +2 more sources
Macromolecular Crowding Enhances the Detection of DNA and Proteins by a Solid-State Nanopore
Nano Letters, 2020 Nanopore analysis of nucleic acid is now routine, but detection of proteins remains challenging. Here, we report the systematic characterization of the effect of macromolecular crowding on the detection sensitivity of a solid-state nanopore for circular ...
Sheena E Radford +2 more
exaly +2 more sources