Physicochemical cues are not potent regulators of human dermal fibroblast trans-differentiation
Biomaterials and Biosystems, 2023 Due to their inherent plasticity, dermal fibroblasts hold great promise in regenerative medicine. Although biological signals have been well-established as potent regulators of dermal fibroblast function, it is still unclear whether physiochemical cues ...
Christina N.M. Ryan +9 more
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Cell-free protein expression under macromolecular crowding conditions. [PDF]
PLoS ONE, 2011 BackgroundCell-free protein expression (CFPE) comprised of in vitro transcription and translation is currently manipulated in relatively dilute solutions, in which the macromolecular crowding effects present in living cells are largely ignored.
Xumeng Ge, Dan Luo, Jianfeng Xu
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Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior. [PDF]
PLoS ONE, 2012 Microenvironments of biological cells are dominated in vivo by macromolecular crowding and resultant excluded volume effects. This feature is absent in dilute in vitro cell culture.
Adam S Zeiger +4 more
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Macromolecular Crowding Regulates the Gene Expression Profile by Limiting Diffusion. [PDF]
PLoS Computational Biology, 2016 We seek to elucidate the role of macromolecular crowding in transcription and translation. It is well known that stochasticity in gene expression can lead to differential gene expression and heterogeneity in a cell population.
Mahdi Golkaram +3 more
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Hyaluronic Acid as Macromolecular Crowder in Equine Adipose-Derived Stem Cell Cultures
Cells, 2021 The use of macromolecular crowding in the development of extracellular matrix-rich cell-assembled tissue equivalents is continuously gaining pace in regenerative engineering.
Sergio Garnica-Galvez +6 more
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The Role of Crowding Forces in Juxtaposing β-Globin Gene Domain Remote Regulatory Elements in Mouse Erythroid Cells. [PDF]
PLoS ONE, 2015 The extremely high concentration of macromolecules in a eukaryotic cell nucleus indicates that the nucleoplasm is a crowded macromolecular solution in which large objects tend to gather together due to crowding forces.
Arkadiy K Golov +2 more
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Effects of Macromolecular Crowding on Genetic Networks [PDF]
Biophysical Journal, 2011 The intracellular environment is crowded with proteins, DNA, and other macromolecules. Under physiological conditions, macromolecular crowding can alter both molecular diffusion and the equilibria of bimolecular reactions and therefore is likely to have a significant effect on the function of biochemical networks.
M.J. Morelli +2 more
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Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions [PDF]
Chemical ReviewsBegoña Monterroso +2 more
exaly +2 more sources
eLife, 2020 Cellular aging is a multifactorial process that is characterized by a decline in homeostatic capacity, best described at the molecular level. Physicochemical properties such as pH and macromolecular crowding are essential to all molecular processes in ...
Sara N Mouton +9 more
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The contrasting effect of macromolecular crowding on amyloid fibril formation. [PDF]
PLoS ONE, 2012 Amyloid fibrils associated with neurodegenerative diseases can be considered biologically relevant failures of cellular quality control mechanisms.
Qian Ma +7 more
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