Macromolecular crowding and decellularization method increase the growth factor binding potential of cell-secreted extracellular matrices [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Recombinant growth factors are used in tissue engineering to stimulate cell proliferation, migration, and differentiation. Conventional methods of growth factor delivery for therapeutic applications employ large amounts of these bioactive cues. Effective,
Shierly W. Fok +6 more
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Comparative analysis of different macromolecular crowding agents in human tenocyte cultures [PDF]
Biomaterials and BiosystemsTendon engineered substitutes remain clinically unavailable, largely due to the need for prolonged culture periods to allow for sufficient extracellular matrix deposition.
Andrea Rossoni +3 more
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Cell wall damage increases macromolecular crowding effects in the Escherichia coli cytoplasm [PDF]
iScience, 2023 Summary: The intracellular milieu is crowded with biomacromolecules. Macromolecular crowding changes the interactions, diffusion, and conformations of biomacromolecules.
Theodoros Pittas +2 more
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A Comparative Study on Cyanine Dyestuffs as Sensor Candidates for Macromolecular Crowding In Vitro and In Vivo [PDF]
Biosensors, 2023 Pseudo isocyanine chloride (PIC) has been identified in a preceding work as a sensor suited to probe macromolecular crowding both in test tubes with solutions of synthetic crowding agents and in HeLa cells as a representative of living systems.
Leon Koch +3 more
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Gums as Macromolecular Crowding Agents in Human Skin Fibroblast Cultures [PDF]
LifeEven though tissue-engineered medicines are under intense academic, clinical, and commercial investigation, only a handful of products have been commercialised, primarily due to the costs associated with their prolonged manufacturing.
Salome Guillaumin +2 more
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Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture [PDF]
Cells, 2022 The absence of a native extracellular matrix and the use of xenogeneic sera are often associated with rapid tenocyte function losses during in vitro culture.
Andrea Rampin +6 more
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Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis [PDF]
Materials Today BioAlthough human tenocytes and dermal fibroblasts have shown promise in tendon engineering, no tissue engineered medicine has been developed due to the prolonged ex vivo time required to develop an implantable device.
Adrian Djalali-Cuevas +11 more
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Localization of protein aggregation in Escherichia coli is governed by diffusion and nucleoid macromolecular crowding effect. [PDF]
PLoS Computational Biology, 2013 Aggregates of misfolded proteins are a hallmark of many age-related diseases. Recently, they have been linked to aging of Escherichia coli (E. coli) where protein aggregates accumulate at the old pole region of the aging bacterium.
Anne-Sophie Coquel +8 more
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Macromolecular crowding and supersaturation protect hemodialysis patients from the onset of dialysis-related amyloidosis [PDF]
Nature Communications, 2022 Amyloid fibrils of β2-microglobulin (β2m) can cause dialysis-related amyloidosis. Here, the authors show that a decrease in serum albumin levels in long-term dialysis deteriorates the inhibitory effects of serum milieux on supersaturation-limited amyloid
Kichitaro Nakajima +13 more
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In vitro and in vivo assessment of a non-animal sourced chitosan scaffold loaded with xeno-free umbilical cord mesenchymal stromal cells cultured under macromolecular crowding conditions [PDF]
Biomaterials and BiosystemsThere is an increasing demand to not only accelerate the development of advanced therapy tissue engineered medicines, but to also eliminate xenogeneic materials from their development cycle.
Alessia Di Nubila +4 more
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