Comparative analysis of different macromolecular crowding agents in human tenocyte cultures [PDF]
Biomaterials and BiosystemsTendon engineered substitutes remain clinically unavailable, largely due to the need for prolonged culture periods to allow for sufficient extracellular matrix deposition.
Andrea Rossoni +3 more
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Liposome-Encapsulated <i>Escherichia coli</i> Lysates to Reconstitute Intracellular Macromolecular Crowding Effects. [PDF]
ACS Synth BiolIntracellular macromolecular crowding impacts biomacromolecule behavior, including oligomerization, phase separation, and diffusion. However, understanding crowding effects in cells is challenging as cells respond and adapt to perturbations.
Kalacheva MS, da Silva NR, Boersma AJ.
europepmc +4 more sources
PEG-mCherry interactions beyond classical macromolecular crowding. [PDF]
Protein SciThe dense cellular environment influences bio-macromolecular structure, dynamics, interactions and function. Despite advancements in understanding protein-crowder interactions, predicting their precise effects on protein structure and function remains ...
Haas-Neill L +3 more
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RNA: The Unsuspected Conductor in the Orchestra of Macromolecular Crowding. [PDF]
Chem RevThis comprehensive Review delves into the chemical principles governing RNA-mediated crowding events, commonly referred to as granules or biological condensates. We explore the pivotal role played by RNA sequence, structure, and chemical modifications in
Zacco E +10 more
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Probing macromolecular crowding at the lipid membrane interface with genetically-encoded sensors. [PDF]
Protein Sci, 2023 Biochemical processes within the living cell occur in a highly crowded environment. The phenomenon of macromolecular crowding is not an exclusive feature of the cytoplasm and can be observed in the densely protein-packed, nonhomogeneous cellular ...
Löwe M +5 more
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Understanding the Impacts of Molecular and Macromolecular Crowding Agents on Protein-Polymer Complex Coacervates. [PDF]
Biomacromolecules, 2023 Complex coacervation refers to the liquid–liquid phase separation (LLPS) process occurring between charged macromolecules. The study of complex coacervation is of great interest due to its implications in the formation of membraneless organelles (MLOs ...
Biswas S +5 more
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Cell wall damage increases macromolecular crowding effects in the Escherichia coli cytoplasm [PDF]
iScience, 2023 Summary: The intracellular milieu is crowded with biomacromolecules. Macromolecular crowding changes the interactions, diffusion, and conformations of biomacromolecules.
Theodoros Pittas +2 more
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Condensed-phase signaling can expand kinase specificity and respond to macromolecular crowding. [PDF]
Mol Cell, 2022 SUMMARY Phase separation can concentrate biomolecules and accelerate reactions. However, the mechanisms and principles connecting this mesoscale organization to signaling dynamics are difficult to dissect because of the pleiotropic effects associated ...
Sang D +5 more
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A Comparative Study on Cyanine Dyestuffs as Sensor Candidates for Macromolecular Crowding In Vitro and In Vivo [PDF]
Biosensors, 2023 Pseudo isocyanine chloride (PIC) has been identified in a preceding work as a sensor suited to probe macromolecular crowding both in test tubes with solutions of synthetic crowding agents and in HeLa cells as a representative of living systems.
Leon Koch +3 more
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Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions. [PDF]
Chem RevMacromolecular crowding affects the activity of proteins and functional macromolecular complexes in all cells, including bacteria. Crowding, together with physicochemical parameters such as pH, ionic strength, and the energy status, influences the ...
Monterroso B +5 more
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