Stress‐dependent macromolecular crowding in the mitochondrial matrix [PDF]
EMBO Journal, 2023 Macromolecules of various sizes induce crowding of the cellular environment. This crowding impacts on biochemical reactions by increasing solvent viscosity, decreasing the water‐accessible volume and altering protein shape, function, and interactions ...
Peter H G M Willems +2 more
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Gums as Macromolecular Crowding Agents in Human Skin Fibroblast Cultures [PDF]
LifeEven though tissue-engineered medicines are under intense academic, clinical, and commercial investigation, only a handful of products have been commercialised, primarily due to the costs associated with their prolonged manufacturing.
Salome Guillaumin +2 more
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Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis [PDF]
Materials Today BioAlthough human tenocytes and dermal fibroblasts have shown promise in tendon engineering, no tissue engineered medicine has been developed due to the prolonged ex vivo time required to develop an implantable device.
Adrian Djalali-Cuevas +11 more
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Macromolecular crowding and decellularization method increase the growth factor binding potential of cell-secreted extracellular matrices [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Recombinant growth factors are used in tissue engineering to stimulate cell proliferation, migration, and differentiation. Conventional methods of growth factor delivery for therapeutic applications employ large amounts of these bioactive cues. Effective,
Shierly W. Fok +6 more
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Underestimated role of macromolecular crowding in bioengineered <i>in vitro</i> models of health and diseases. [PDF]
Mater Today BioMacromolecular crowding (MMC) is a ubiquitous phenomenon in biological systems that is largely overlooked in bioengineered in vitro cellular models. This comprehensive review examines the significant impact of both intracellular and extracellular MMC on ...
Lagrange J +4 more
europepmc +3 more sources
Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture [PDF]
Cells, 2022 The absence of a native extracellular matrix and the use of xenogeneic sera are often associated with rapid tenocyte function losses during in vitro culture.
Andrea Rampin +6 more
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High Macromolecular Crowding in Liposomes from Microfluidics [PDF]
Advanced Science, 2022 The intracellular environment is crowded with macromolecules that influence biochemical equilibria and biomacromolecule diffusion. The incorporation of such crowding in synthetic cells would be needed to mimic the biochemistry of living cells.
Arnold J Boersma +2 more
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In vitro and in vivo assessment of a non-animal sourced chitosan scaffold loaded with xeno-free umbilical cord mesenchymal stromal cells cultured under macromolecular crowding conditions [PDF]
Biomaterials and BiosystemsThere is an increasing demand to not only accelerate the development of advanced therapy tissue engineered medicines, but to also eliminate xenogeneic materials from their development cycle.
Alessia Di Nubila +4 more
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Macromolecular Crowding and DNA: Bridging the Gap between In Vitro and In Vivo. [PDF]
Int J Mol Sci, 2023 The cellular environment is highly crowded, with up to 40% of the volume fraction of the cell occupied by various macromolecules. Most laboratory experiments take place in dilute buffer solutions; by adding various synthetic or organic macromolecules ...
Collette D, Dunlap D, Finzi L.
europepmc +2 more sources
Can Macromolecular Crowding Help Regulate Glutamate Dehydrogenase Activity? [PDF]
ACS OmegaGenesis Rosario +8 more
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