Results 11 to 20 of about 103,280 (234)
Cell-free protein expression under macromolecular crowding conditions. [PDF]
PLoS ONE, 2011 BackgroundCell-free protein expression (CFPE) comprised of in vitro transcription and translation is currently manipulated in relatively dilute solutions, in which the macromolecular crowding effects present in living cells are largely ignored.
Xumeng Ge, Dan Luo, Jianfeng Xu
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Effects of Macromolecular Crowding on Genetic Networks [PDF]
Biophysical Journal, 2011 The intracellular environment is crowded with proteins, DNA, and other macromolecules. Under physiological conditions, macromolecular crowding can alter both molecular diffusion and the equilibria of bimolecular reactions and therefore is likely to have ...
Allen, R.J. +5 more
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, 2020 Biokemijski procesi unutar stanica odvijaju se u uvjetima makromolekularne zgusnutosti. Makromolekularna zgusnutost, poznata kao efekt isključenog volumena, definira se kao međusobni utjecaj staničnih molekula jednih na druge.
Hrsan, Jana
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Macromolecular Crowding Regulates the Gene Expression Profile by Limiting Diffusion. [PDF]
PLoS Computational Biology, 2016 We seek to elucidate the role of macromolecular crowding in transcription and translation. It is well known that stochasticity in gene expression can lead to differential gene expression and heterogeneity in a cell population.
Mahdi Golkaram +3 more
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Guiding Protein Aggregation with Macromolecular Crowding [PDF]
Biochemistry, 2008 Macromolecular crowding is expected to have a significant effect on protein aggregation. In the present study we analyzed the effect of macromolecular crowding on fibrillation of four proteins, bovine S-carboxymethyl-α-lactalbumin (a disordered form of ...
Fink, Anthony L. +3 more
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The Differential Response of Proteins to Macromolecular Crowding. [PDF]
PLoS Computational Biology, 2016 The habitat in which proteins exert their function contains up to 400 g/L of macromolecules, most of which are proteins. The repercussions of this dense environment on protein behavior are often overlooked or addressed using synthetic agents such as poly(
Michela Candotti, Modesto Orozco
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A Sensor for Quantification of Macromolecular Crowding in Living Cells [PDF]
Biophysical Journal, 2015 Macromolecular crowding in cells influences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and ...
Zuhorn, Inge S. +7 more
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The contrasting effect of macromolecular crowding on amyloid fibril formation. [PDF]
PLoS ONE, 2012 Amyloid fibrils associated with neurodegenerative diseases can be considered biologically relevant failures of cellular quality control mechanisms.
Qian Ma +7 more
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Macromolecular crowding in Rhodobacter sphaeroides [PDF]
, 2021 Macromolecular crowding is thought to have an important role in protein dynamics and molecular diffusion in bacteria. However, the dynamic and heterogeneous nature of the cytoplasm makes it challenging to study and test the significance of crowding in ...
Khoo, Jia
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Effects of macromolecular crowding on protein conformational changes. [PDF]
PLoS Computational Biology, 2010 Many protein functions can be directly linked to conformational changes. Inside cells, the equilibria and transition rates between different conformations may be affected by macromolecular crowding.
Hao Dong, Sanbo Qin, Huan-Xiang Zhou
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